Dr Romain Rouet

Christ D, 2018, 'Expression of IgG monoclonals with engineered immune effector functions', in Nevoltris D; Chames P (ed.), Antibody Engineering. Methods in Molecular Biology, Humana Press, New York, NY, pp. 313 - 334, http://dx.doi.org/10.1007/978-1-4939-8648-4_16

Abayasingam A; Balachandran H; Agapiou D; Hammoud M; Rodrigo C; Keoshkerian E; Li H; Brasher NA; Christ D; Rouet R; Burnet D; Grubor-Bauk B; Rawlinson W; Turville S; Aggarwal A; Stella AO; Fichter C; Brilot F; Mina M; Post JJ; Hudson B; Gilroy N; Dwyer D; Sasson SC; Tea F; Pilli D; Kelleher A; Tedla N; Lloyd AR; Martinello M; Bull RA, 2021, 'Long-term persistence of RBD⁺ memory B cells encoding neutralizing antibodies in SARS-CoV-2 infection', Cell Reports Medicine, vol. 2, pp. 100228, http://dx.doi.org/10.1016/j.xcrm.2021.100228

Rouet R; Mazigi O; Walker GJ; Langley DB; Sobti M; Schofield P; Lenthall H; Jackson J; Ubiparipovic S; Henry JY; Abayasingam A; Burnett D; Kelleher A; Brink R; Bull RA; Turville S; Stewart AG; Goodnow CC; Rawlinson WD; Christ D, 2021, 'Potent SARS-CoV-2 binding and neutralization through maturation of iconic SARS-CoV-1 antibodies', mAbs, vol. 13, pp. 1922134, http://dx.doi.org/10.1080/19420862.2021.1922134

Brown P; Zhou Y; Tan AC; El-Esawi MA; Liehr T; Blanck O; Gladue DP; Almeida GMF; Cernava T; Sorzano CO; Yeung AWK; Engel MS; Chandrasekaran AR; Muth T; Staege MS; Daulatabad SV; Widera D; Zhang J; Meule A; Honjo K; Pourret O; Yin CC; Zhang Z; Cascella M; Flegel WA; Goodyear CS; van Raaij MJ; Bukowy-Bieryllo Z; Campana LG; Kurniawan NA; Lalaouna D; Hüttner FJ; Ammerman BA; Ehret F; Cobine PA; Tan EC; Han H; Xia W; McCrum C; Dings RPM; Marinello F; Nilsson H; Nixon B; Voskarides K; Yang L; Costa VD; Bengtsson-Palme J; Bradshaw W; Grimm DG; Kumar N; Martis E; Prieto D; Sabnis SC; Amer SEDR; Liew AWC; Perco P; Rahimi F; Riva G; Zhang C; Devkota HP; Ogami K; Basharat Z; Fierz W; Siebers R; Tan KH; Boehme KA; Brenneisen P; Brown JAL; Dalrymple BP; Harvey DJ; Ng G; Werten S; Bleackley M; Dai Z; Dhariwal R; Gelfer Y; Hartmann MD; Miotla P; Tamaian R; Govender P; Gurney-Champion OJ; Kauppila JH; Zhang X; Echeverría N; Subhash S; Sallmon H; Tofani M; Bae T; Bosch O; Cuív PO; Danchin A; Diouf B; Eerola T; Evangelou E; Filipp F; Klump H; Kurgan L; Smith SS; Terrier O; Tuttle N, 2019, 'Large expert-curated database for benchmarking document similarity detection in biomedical literature search', Database, vol. 2019, pp. 1 - 67, http://dx.doi.org/10.1093/database/baz085

Luthra A; Langley DB; Schofield P; Jackson J; Abdelatti M; Rouet R; Nevoltris D; Mazigi O; Crossett B; Christie M; Christ D, 2019, 'Human Antibody Bispecifics through Phage Display Selection', Biochemistry, vol. 58, pp. 1701 - 1704, http://dx.doi.org/10.1021/acs.biochem.9b00037

Vennin C; Mélénec P; Rouet R; Nobis M; Cazet AS; Murphy KJ; Herrmann D; Reed DA; Lucas MC; Warren SC; Elgundi Z; Pinese M; Kalna G; Roden D; Samuel M; Zaratzian A; Grey ST; Da Silva A; Leung W; Johns AL; Chantrill LA; Chou A; Steinmann A; Arshi M; Dwarte T; Froio D; Pereira B; Ritchie S; Chambers CR; Metcalf X; Waddell N; Pearson JV; Patch AM; Nones K; Newell F; Mukhopadhyay P; Addala V; Kazakoff S; Holmes O; Leonard C; Wood S; Grimmond SM; Hofmann O; Christ A; Bruxner T; Samra JS; Pavlakis N; High HA; Asghari R; Merrett ND; Pavey D; Das A; Cosman PH; Ismail K; O’Connnor C; Stoita A; Williams D; Spigellman A; Lam VW; McLeod D; Kirk J; Kench JG; Grimison P; Cooper CL; Sandroussi C; Goodwin A; Mead RS; Tucker K; Andrews L; Texler M; Forest C; Epari KP; Ballal M; Fletcher DR; Mukhedkar S; Zeps N; Beilin M; Feeney K; Nguyen NQ; Ruszkiewicz AR; Worthley C; Chen J; Brooke-Smith ME; Papangelis V; Clouston AD; Barbour AP; O’Rourke TJ; Fawcett JW; Slater K; Hatzifotis M; Hodgkinson P; Nikfarjam M; Eshleman JR; Hruban RH; Wolfgang CL; Lawlor RT; Beghelli S; Corbo V; Scardoni M; Bassi C, 2019, 'CAF hierarchy driven by pancreatic cancer cell p53-status creates a pro-metastatic and chemoresistant environment via perlecan', Nature Communications, vol. 10, pp. 3637, http://dx.doi.org/10.1038/s41467-019-10968-6

Rouet R; Christ D, 2019, 'Efficient Intracellular Delivery of CRISPR-Cas Ribonucleoproteins through Receptor Mediated Endocytosis', ACS Chemical Biology, vol. 14, pp. 554 - 561, http://dx.doi.org/10.1021/acschembio.9b00116

Zeraati M; Langley DB; Schofield P; Moye AL; Rouet R; Hughes WE; Bryan TM; Dinger ME; Christ D, 2018, 'I-motif DNA structures are formed in the nuclei of human cells', Nature Chemistry, vol. 10, pp. 631 - 637, http://dx.doi.org/10.1038/s41557-018-0046-3

Rouet R; Jackson KJL; Langley DB; Christ D, 2018, 'Next-generation sequencing of antibody display repertoires', Frontiers in Immunology, vol. 9, http://dx.doi.org/10.3389/fimmu.2018.00118

Fernandez JP; Vejnar CE; Giraldez AJ; Rouet R; Moreno-Mateos MA, 2018, 'Optimized CRISPR-Cpf1 system for genome editing in zebrafish', Methods, vol. 150, pp. 11 - 18, http://dx.doi.org/10.1016/j.ymeth.2018.06.014

Rouet R; de Onate L; Li J; Murthy N; Wilson RC, 2018, 'Engineering CRISPR-Cas9 RNA-Protein Complexes for Improved Function and Delivery', CRISPR JOURNAL, vol. 1, pp. 367 - 378, http://dx.doi.org/10.1089/crispr.2018.0037

Roy M; Rouet R; Thuma B; Lintner N; Rubitski D; Finley J; Wisniewska H; Mendonsa R; Oñate L; Hirsh A; Barrón JC; McLellan T; Bellenger J; Feng X; Varghese A; Chrunyk B; Borzilleri K; Hesp K; Zhou K; Ma N; Tu M; Wilson R; Dullea R; McClure K; Liras S; Mascitti V; Doudna J, 2018, 'Receptor‐Mediated Delivery of CRISPR‐Cas9 Endonuclease for Cell Type Specific Gene Editing', The FASEB Journal, vol. 32, pp. 649.2 - 649.2, http://dx.doi.org/10.1096/fasebj.2018.32.1_supplement.649.2

Rouet R; Thuma BA; Roy MD; Lintner NG; Rubitski DM; Finley JE; Wisniewska HM; Mendonsa R; Hirsh A; De Oñate L; Compte Barrón J; McLellan TJ; Bellenger J; Feng X; Varghese A; Chrunyk BA; Borzilleri K; Hesp KD; Zhou K; Ma N; Tu M; Dullea R; McClure KF; Wilson RC; Liras S; Mascitti V; Doudna JA, 2018, 'Receptor-Mediated Delivery of CRISPR-Cas9 Endonuclease for Cell-Type-Specific Gene Editing', Journal of the American Chemical Society, vol. 140, pp. 6596 - 6603, http://dx.doi.org/10.1021/jacs.8b01551

Moreno-Mateos MA; Fernandez JP; Rouet R; Vejnar CE; Lane MA; Mis E; Khokha MK; Doudna JA; Giraldez AJ, 2017, 'CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing', Nature Communications, vol. 8, http://dx.doi.org/10.1038/s41467-017-01836-2

Rouet R; Langley DB; Schofield P; Christie M; Roome B; Porebski BT; Buckle AM; Clifton BE; Jackson CJ; Stock D; Christ D, 2017, 'Structural reconstruction of protein ancestry', Proceedings of the National Academy of Sciences of the United States of America, vol. 114, pp. 3897 - 3902, http://dx.doi.org/10.1073/pnas.1613477114

Seow J; Morales RAV; MacRaild CA; Krishnarjuna B; McGowan S; Dingjan T; Jaipuria G; Rouet R; Wilde KL; Atreya HS; Richards JS; Anders RF; Christ D; Drinkwater N; Norton RS, 2017, 'Structure and Characterisation of a Key Epitope in the Conserved C-Terminal Domain of the Malaria Vaccine Candidate MSP2', Journal of Molecular Biology, vol. 429, pp. 836 - 846, http://dx.doi.org/10.1016/j.jmb.2017.02.003

Rouet R; Dudgeon K; Christie M; Langley D; Christ D, 2015, 'Fully human VH single domains that rival the stability and cleft recognition of camelid antibodies', Journal of Biological Chemistry, vol. 290, pp. 11905 - 11917, http://dx.doi.org/10.1074/jbc.M114.614842

Morales RAV; Macraild CA; Seow J; Krishnarjuna B; Drinkwater N; Rouet R; Anders RF; Christ D; McGowan S; Norton RS, 2015, 'Structural basis for epitope masking and strain specificity of a conserved epitope in an intrinsically disordered malaria vaccine candidate', Scientific Reports, vol. 5, http://dx.doi.org/10.1038/srep10103

Sabouri Z; Schofield P; Horikawa K; Spierings E; Kipling D; Randall KL; Langley D; Roome B; Vazquez-Lombardi R; Rouet R; Hermes J; Chan TD; Brink R; Dunn-Walters DK; Christ D; Goodnow CC, 2014, 'Redemption of autoantibodies on anergic B cells by variable-region glycosylation and mutation away from self-reactivity', Proceedings of the National Academy of Sciences of the United States of America, vol. 111, http://dx.doi.org/10.1073/pnas.1406974111

Rouet R; Lowe D; Christ D, 2014, 'Stability engineering of the human antibody repertoire', FEBS Letters, vol. 588, pp. 269 - 277, http://dx.doi.org/10.1016/j.febslet.2013.11.029

Swift J; Saing S; Rouet R; Dudgeon K; Schofield P; Sewell W; Christ D; James L, 2014, 'Identification of aggregation inhibitors of the human antibody light chain repertoire by phage display', Protein Engineering, Design and Selection, vol. 27, pp. 405 - 409, http://dx.doi.org/10.1093/protein/gzu026

Rouet R; Christ D, 2014, 'Bispecific antibodies with native chain structure', Nature Biotechnology, vol. 32, pp. 136 - 137, http://dx.doi.org/10.1038/nbt.2812

Dudgeon K; Rouet R; Christ D, 2013, 'Rapid prediction of expression and refolding yields using phage display', Protein Engineering, Design and Selection, vol. 26, pp. 671 - 674, http://dx.doi.org/10.1093/protein/gzt019

Dudgeon K; Rouet R; Kokmeijer I; Schofield P; Stolp J; Langley D; Stock D; Christ DU, 2012, 'General strategy for the generation of human antibody variable domains with increased aggregation resistance', Proceedings of the National Academy of Sciences of the United States of America, vol. 109, pp. 10879 - 10884, http://dx.doi.org/10.1073/pnas.1202866109

Rouet R; Lowe D; Dudgeon K; Roome B; Schofield P; Langley D; Andrews J; Whitfeld P; Jermutus L; Christ D, 2012, 'Expression of high-affinity human antibody fragments in bacteria', Nature Protocols, vol. 7, pp. 364 - 373, http://dx.doi.org/10.1038/nprot.2011.448

Rouet R; Dudgeon K; Christ DU, 2012, 'Generation of human single domain antibody repertoires by kunkel mutagenesis', Methods in Molecular Biology, vol. 907, pp. 195 - 209, http://dx.doi.org/10.1007/978-1-61779-974-7-10

Dudgeon K; Rouet R; Famm K; Christ D, 2012, 'Selection of human VH single domains with improved biophysical properties by phage display.', Methods in molecular biology (Clifton, N.J.), vol. 911, pp. 383 - 397

Rouet R; Dudgeon K; Christ D, 2012, 'Generation of human single domain antibody repertoires by Kunkel mutagenesis.', Methods Mol Biol, vol. 907, pp. 195 - 209, http://dx.doi.org/10.1007/978-1-61779-974-7_10