The Electron Microscopy research group (EMRG) generate ultrastructural and immunocytochemical data from surgical, biopsy tissue and cell culture samples at a resolution typically of 0.5 nm. Electron Microscopy is particularly valuable for visualising pathological changes and structures at the sub-cellular or organelle level and is traditionally used for the delineation of disease pathogenesis. Cryogenic sample fixation has enabled studies of single proteins and viral substructures at near-atomic resolution in structural biology.
Electron Microscopy platforms also may be configured for (i) 3D and wide area 2D studies (ii) elemental analysis using technologies such as Energy Dispersive Spectroscopy (EDS), (iii) spatial biomarker quantification and (iv) correlative light and electron microscopy (CLEM). The EMRG is a longstanding collaboration between the Ingham Institute, NSW Health Pathology, Western Sydney University and the Mark Wainwright Analytical Centre.
The goal of the Electron Microscopy Research Group (EMRG) is to develop new approaches to the visualisation of cells and subcelluar fine structure for pathology diagnostics and cell biology research. These new approaches can embrace novel fixation and sample preparation protocols such as freeze substitution, nanoparticle immunocytochemical markers for correlation studies, computer-based quantitative image analysis, 3D structural rendering and new electron column designs such as for low kV STEM desktop systems. Much of this work is done at the Ingham Institute and includes:
NSW Health Pathology, Ingham Institute
Electron Microscope Unit, Mark Wainwright Analytical Centre
External links and affiliations: