Dr Jai   Tree
Senior Lecturer

Dr Jai Tree

Science
Biotech & Biomolecular Science

Research program

The lab is interested in how bacteria regulate gene expression with a focus on understanding the contribution of non-coding RNAs. It is now apparent that the genomes of all organisms are transcribed into an array of regulatory non-coding RNAs (ncRNAs) and bacterial pathogens have not escaped the ncRNA revolution. Our major challenge now is to understand the functions of these RNA species and high throughput sequencing technologies and bioinformatics are providing the tools that will allow us to address these questions.

Regulatory RNAs and pathogenesis 

Our model system is the human pathogen, enterohaemorrhagic E. coli O157 (EHEC), that causes disease ranging from gastroenteritis to life treating haemolytic uremic syndrome. The later is caused by release of Shiga toxins that are expressed from bacteriophage (bacterial viruses) that have inserted into the bacterial genome. We have recently identified large numbers of non-coding RNAs that are encoded in EHEC and are seeking to identify those that regulate virulence. We anticipate that by understanding how bacteria employ ncRNAs to control gene expression and respond to stress we will be able to design interventions that limit bacterial pathogenesis and dissemination.

Regulatory RNAs and antibiotic resistance 

Non-coding RNAs play prominent roles in controlling the composition of the bacterial cell wall and membranes. A number of ncRNAs have been shown to control intrinsic antibiotic resistance in bacterial pathogens with 'urgent' or 'serious' levels of antimicrobial resistance. Non-coding RNAs contribute to antibiotic resistance in Methicillin-resistant Staphylococcus aureus (MRSA, designated a 'serious' threat) and we are using high-through put sequencing technologies, bioinformatics, and molecular biology to identify key ncRNAs that mediate antibiotic resistance in MRSA.  

More information on the lab's research can be found on the Tree Lab website.

Selected recent publications

  1. Early termination of the Shiga toxin transcript generates a regulatory small RNA. Sy BM, Lan R, Tree JJPNAS. 2020117 (40) 25055-25065
  2. Networks of control: small RNA control of antibiotic resistance. Mediati DG, Wu S, Wu W, Tree JJ. 2020. Trends in Geneticsin press.
  3. Hfq CLASH uncovers sRNA-target interaction networks linked to nutrient availability adaptation. Iosub IA, van Nues RW, McKellar SW, et al., Tree JJ, Viero G, Granneman S. eLife. 2020. 9:e54655.
  4. An RNA-dependent mechanism for transient expression of bacterial translocation filaments. Wang D, McAteer SP, Wawszczyk AB, Russell CD, Tahoun A, Elmi A, Cockroft SL, Tollervey D, Granneman S, Tree JJ*, Gally DL*. 2018. Nucleic Acids Research. *co-corresponding authors 
  5. Ribosome maturation by the endoribonuclease YbeY stabilises a type III secretion system transcript required for virulence of enterohemorrhagic Escherichia coli. McAteer SP, Sy BM, Wong JL, Tollervey D, Gally DL*, Tree JJ*Journal of Biological Chemistry. 2018. *co-corresponding authors 
  6. Small RNA interactome of pathogenic E. coli revealed through crosslinking of RNase E. Waters SA, McAteer SP, Kudla G, Pang I, Deshpande NP, Amos TG, Leong KW, Wilkins MR, Strugnell R, Gally DL, Tollervey D, Tree JJ. 2017 Feb 1;36(3):374-387. EMBO Journal.
  7. Identification of bacteriophage-encoded anti-sRNAs in pathogenic Escherichia coli Tree JJ, Granneman S, McAteer SP, Tollervey D, Gally DL. 2014. 55(2):199-213. Molecular Cell.

Professional Experience

  • 2015-current Senior Lecturer, School of Biotech & Biomolecular Sciences, UNSW
  • 2014-2015 Post Doctoral Researcher, Peter Doherty Institute, University of Melbourne
  • 2011-2014 Post-doctoral Researcher, Wellcome Centre for Cell Biology & The Roslin Institute, University of Edinburgh, UK
  • 2007-2011 Post-doctoral Researcher, Royal (Dick) School of Veterinary Medicine & The Roslin Institute, University of Edinburgh, UK
  • 2007 PhD Microbiology, University of Queensland
  • 2001 BScApp (Biotech) Hons I, University of Queensland

Teaching

Coordinator: 

  • Microbiology 1 MICR2011

Lecturer:

  • Microbiology 1 MICR2011
  • Bacteria and Disease BABS 3081
  • Microbial Genetics BABS3021
  • Medicine Foundations MFAC1501
  • Molecular Frontiers BABS3281

Supervision

PhD, Honours, and 3rd year Undergraduate projects in Molecular Biology and Microbiology are available in the lab. 

Current Lab members:

  • Dr Daniel Mediati (Post-doctoral researcher)
  • Dr Brandon Sy (Post-docotral researcher)
  • Dr Ignatius Pang (Post-doctoral researcher)
  • Winton Wu (PhD student)
  • Sylvania Wu (PhD student)
  • Daniel Neville (Honours student)
Phone
+612 90652366
Location
Office: Rm 3113, Biosciences South Building E26 Lab: Rm 3005, Biosciences South Building E26

Publications

  • Book Chapters | 2018
    Dr Jai Tree
    Sy B; Wong J; Granneman S; Tollervey D; Gally D; Tree JJ, 2018, 'High-resolution, high-throughput analysis of Hfq-binding sites using UV crosslinking and analysis of cDNA (CRAC)', in Bacterial regulatory RNA, pp. 251 - 272, http://dx.doi.org/10.1007/978-1-4939-7634-8_15
    Book Chapters | 2018
    Dr Jai Tree
    Wong J; Pang I; Wilkins M; Tree J, 2018, 'Systems-level analysis of bacterial regulatory small RNA networks', in Systems Biology, Springer, pp. 97 - 127
    Books | 2018
    Dr Jai Tree
    2018, Systems Biology, Springer International Publishing, http://dx.doi.org/10.1007/978-3-319-92967-5
  • Journal articles | 2021
    Dr Jai Tree
    Mediati DG; Wu S; Wu W; Tree JJ, 2021, 'Networks of Resistance: Small RNA Control of Antibiotic Resistance', Trends in Genetics, vol. 37, pp. 35 - 45, http://dx.doi.org/10.1016/j.tig.2020.08.016
    Journal articles | 2021
    Dr Jai Tree
    Sy BM; Tree JJ, 2021, 'Small RNA Regulation of Virulence in Pathogenic Escherichia coli', Frontiers in Cellular and Infection Microbiology, vol. 10, http://dx.doi.org/10.3389/fcimb.2020.622202
    Journal articles | 2020
    Dr Jai Tree
    Gao W; Guérillot R; Lin YH; Tree J; Beaume M; François P; Monk IR; Seemann T; Schrenzel J; Howden BP; Stinear TP, 2020, 'Comparative Transcriptomic and Functional Assessments of Linezolid-Responsive Small RNA Genes in Staphylococcus aureus', mSystems, vol. 5, http://dx.doi.org/10.1128/mSystems.00665-19
    Journal articles | 2020
    Dr Jai Tree
    Iosub IA; van Nues RW; McKellar SW; Nieken KJ; Marchioretto M; Sy B; Tree JJ; Viero G; Granneman S, 2020, 'Hfq CLASH uncovers sRNA-target interaction networks linked to nutrient availability adaptation', eLife, vol. 9, pp. 1 - 33, http://dx.doi.org/10.7554/eLife.54655
    Journal articles | 2020
    Dr Jai Tree
    Fernandez-Brando RJ; McAteer SP; Montanez-Culma J; Cortes-Araya Y; Tree J; Bernal A; Fuentes F; Fitzgerald S; Pineda GE; Ramos MV; Gally DL; Palermo MS, 2020, 'Mechanisms involved in the adaptation of Escherichia coli O157:H7 to the host intestinal microenvironment', Clinical Science, vol. 134, pp. 3283 - 3301, http://dx.doi.org/10.1042/CS20200971
    Journal articles | 2020
    Dr Jai Tree
    Sy BM; Lan R; Tree JJ, 2020, 'Early termination of the Shiga toxin transcript generates a regulatory small RNA', Proceedings of the National Academy of Sciences of the United States of America, vol. 117, pp. 25055 - 25065, http://dx.doi.org/10.1073/pnas.2006730117
    Journal articles | 2018
    Dr Jai Tree
    McAteer SP; Sy BM; Wong JL; Tollervey D; Gally DL; Tree JJ, 2018, 'Ribosome maturation by the endoribonuclease YbeY stabilizes a type 3 secretion system transcript required for virulence of enterohemorrhagic Escherichia coli', Journal of Biological Chemistry, vol. 293, pp. 9006 - 9016, http://dx.doi.org/10.1074/jbc.RA117.000300
    Journal articles | 2018
    Dr Jai Tree
    Husna AU; Wang N; Cobbold SA; Newton HJ; Hocking DM; Wilksch JJ; Scott TA; Davies MR; Hinton JC; Tree JJ; Lithgow T; McConville MJ; Strugnell RA, 2018, 'Methionine biosynthesis and transport are functionally redundant for the growth and virulence of Salmonella Typhimurium', Journal of Biological Chemistry, vol. 293, pp. 9506 - 9519, http://dx.doi.org/10.1074/jbc.RA118.002592
    Journal articles | 2018
    Dr Jai Tree
    Wang D; McAteer S; Wawszczyk AB; Russel CD; Tahoun A; Elmi A; Cockroft SL; Tollervey D; Granneman S; Tree J; Gally D, 2018, 'An RNA-dependent mechanism for transient expression of bacterial translocation filaments', Nucliec Acids Research, vol. 46, pp. 3366 - 3381, http://dx.doi.org/10.1093/nar/gky096
    Journal articles | 2018
    Dr Jai Tree
    Tree JJ; Gerdes K; Tollervey D, 2018, 'Transcriptome-Wide Analysis of Protein–RNA and RNA–RNA Interactions in Pathogenic Bacteria', Methods in Enzymology, vol. 612, pp. 467 - 488, http://dx.doi.org/10.1016/bs.mie.2018.08.009
    Journal articles | 2017
    Dr Jai Tree
    Waters SA; McAteer SP; Kudla G; Pang I; Deshpande NP; Amos TG; Leong KW; Wilkins MR; Strugnell R; Gally DL; Tollervey D; Tree JJ, 2017, 'Small RNA interactome of pathogenic E. coli revealed through crosslinking of RNase E', EMBO Journal, vol. 36, pp. 374 - 387, http://dx.doi.org/10.15252/embj.201694639
    Journal articles | 2016
    Dr Jai Tree
    Winther K; Tree J; tollervey D; Gerdes G, 2016, 'VapCs of Mycobacterium tuberculosis cleave RNAs essential for translation', Nucleic Acids Research, vol. 44, pp. 9860 - 9871, http://dx.doi.org/10.1093/nar/gkw781
    Journal articles | 2016
    Dr Jai Tree
    Monk IR; Tree JJ; Howden BP; Stinear TP; Foster TJ, 2016, 'Erratum for Monk et al., Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages.', mBio, vol. 7, pp. e00171, http://dx.doi.org/10.1128/mBio.00171-16
    Journal articles | 2015
    Dr Jai Tree
    Monk IR; Tree JJ; Howden BP; Stinear TP; Foster TJ, 2015, 'Complete bypass of restriction systems for major staphylococcus aureus lineages', mBio, vol. 6, pp. 1 - 12, http://dx.doi.org/10.1128/mBio.00308-15
    Journal articles | 2014
    Dr Jai Tree
    Tree JJ; Granneman S; McAteer SP; Tollervey D; Gally DL, 2014, 'Identification of Bacteriophage-Encoded Anti-sRNAs in Pathogenic Escherichia coli', Molecular Cell, vol. 55, pp. 199 - 213, http://dx.doi.org/10.1016/j.molcel.2014.05.006
    Journal articles | 2012
    Dr Jai Tree
    Flockhart AF; Tree JJ; Xu X; Karpiyevich M; McAteer SP; Rosenblum R; Shaw DJ; Low CJ; Best A; Gannon V; Laing C; Murphy KC; Leong JM; Schneiders T; La Ragione R; Gally DL, 2012, 'Identification of a novel prophage regulator in Escherichia coli controlling the expression of type III secretion', Molecular Microbiology, vol. 83, pp. 208 - 223, http://dx.doi.org/10.1111/j.1365-2958.2011.07927.x
    Journal articles | 2012
    Dr Jai Tree
    Xu X; McAteer SP; Tree JJ; Shaw DJ; Wolfson EBK; Beatson SA; Roe AJ; Allison LJ; Chase-Topping ME; Mahajan A; Tozzoli R; Woolhouse MEJ; Morabito S; Gally DL, 2012, 'Lysogeny with Shiga toxin 2-encoding bacteriophages represses type III secretion in enterohemorrhagic Escherichia coli', PLoS Pathogens, vol. 8, http://dx.doi.org/10.1371/journal.ppat.1002672
    Journal articles | 2011
    Dr Jai Tree
    Tahoun A; Siszler G; Spears K; McAteer S; Tree J; Paxton E; Gillespie TL; Martinez-Argudo I; Jepson MA; Shaw DJ; Koegl M; Haas J; Gally DL; Mahajan A, 2011, 'Comparative analysis of EspF variants in inhibition of Escherichia coli phagocytosis by macrophages and inhibition of E. Coli translocation through human- and bovine-derived M cells', Infection and Immunity, vol. 79, pp. 4716 - 4729, http://dx.doi.org/10.1128/IAI.00023-11
    Journal articles | 2011
    Dr Jai Tree
    Wang D; Zetterström CE; Gabrielsen M; Beckham KSH; Tree JJ; Macdonald SE; Byron O; Mitchell TJ; Gally DL; Herzyk P; Mahajan A; Uvell H; Burchmore R; Smith BO; Elofsson M; Roe AJ, 2011, 'Identification of bacterial target proteins for the salicylidene acylhydrazide Class of virulence-blocking compounds', Journal of Biological Chemistry, vol. 286, pp. 29922 - 29931, http://dx.doi.org/10.1074/jbc.M111.233858
    Journal articles | 2011
    Dr Jai Tree
    Tree JJ; Roe AJ; Flockhart A; Mcateer SP; Xu X; Shaw D; Mahajan A; Beatson SA; Best A; Lotz S; Woodward MJ; La Ragione R; Murphy KC; Leong JM; Gally DL, 2011, 'Transcriptional regulators of the GAD acid stress island are carried by effector protein-encoding prophages and indirectly control type III secretion in enterohemorrhagic Escherichia coli O157:H7', Molecular Microbiology, vol. 80, pp. 1349 - 1365, http://dx.doi.org/10.1111/j.1365-2958.2011.07650.x
    Journal articles | 2011
    Dr Jai Tree
    Bail J; McAteer SP; Paxton E; Mahajan A; Gally DL; Tree JJ, 2011, 'Screening of an E. Coli O157: H7 bacterial artificial chromosome library by comparative genomic hybridization to identify genomic regions contributing to growth in bovine gastrointestinal mucus and epithelial cell colonization', Frontiers in Microbiology, vol. 2, http://dx.doi.org/10.3389/fmicb.2011.00168
    Journal articles | 2010
    Dr Jai Tree
    Allsopp LP; Totsika M; Tree JJ; Ulett GC; Mabbett AN; Wells TJ; Kobe B; Beatson SA; Schembri MA, 2010, 'UpaH is a newly identified autotransporter protein that contributes to biofilm formation and bladder colonization by uropathogenic Escherichia coli CFT073', Infection and Immunity, vol. 78, pp. 1659 - 1669, http://dx.doi.org/10.1128/IAI.01010-09
    Journal articles | 2010
    Dr Jai Tree
    Achard MES; Tree JJ; Holden JA; Simpfendorfer KR; Wijburg OLC; Strugnell RA; Schembri MA; Sweet MJ; Jennings MP; McEwan AG, 2010, 'The multi-copper-ion oxidase CueO of Salmonella enterica serovar typhimurium is required for systemic virulence', Infection and Immunity, vol. 78, pp. 2312 - 2319, http://dx.doi.org/10.1128/IAI.01208-09
    Journal articles | 2009
    Dr Jai Tree
    Tree JJ; Wolfson EB; Wang D; Roe AJ; Gally DL, 2009, 'Controlling injection: regulation of type III secretion in enterohaemorrhagic Escherichia coli', Trends in Microbiology, vol. 17, pp. 361 - 370, http://dx.doi.org/10.1016/j.tim.2009.06.001
    Journal articles | 2009
    Dr Jai Tree
    Tree JJ; Wang D; McInally C; Mahajan A; Layton A; Houghton I; Elofsson M; Stevens MP; Gally DL; Roe AJ, 2009, 'Characterization of the effects of salicylidene acylhydrazide compounds on type III secretion in Escherichia coli O157:H7', Infection and Immunity, vol. 77, pp. 4209 - 4220, http://dx.doi.org/10.1128/IAI.00562-09
    Journal articles | 2009
    Dr Jai Tree
    Mabbett AN; Ulett GC; Watts RE; Tree JJ; Totsika M; Ong CLY; Wood JM; Monaghan W; Looke DF; Nimmo GR; Svanborg C; Schembri MA, 2009, 'Virulence properties of asymptomatic bacteriuria Escherichia coli', International Journal of Medical Microbiology, vol. 299, pp. 53 - 63, http://dx.doi.org/10.1016/j.ijmm.2008.06.003
    Journal articles | 2009
    Dr Jai Tree
    Mahajan A; Currie CG; Mackie S; Tree J; Mcateer S; Mckendrick I; Mcneilly TN; Roe A; La Ragione RM; Woodward MJ; Gally DL; Smith DGE, 2009, 'An investigation of the expression and adhesin function of H7 flagella in the interaction of Escherichia coli O157: H7 with bovine intestinal epithelium', Cellular Microbiology, vol. 11, pp. 121 - 137, http://dx.doi.org/10.1111/j.1462-5822.2008.01244.x
    Journal articles | 2008
    Dr Jai Tree
    Tree JJ; Ulett GC; Ong CLY; Trott DJ; McEwan AG; Schembri MA, 2008, 'Trade-off between iron uptake and protection against oxidative stress: Deletion of cueO promotes uropathogenic Escherichia coli virulence in a mouse model of urinary tract infection', Journal of Bacteriology, vol. 190, pp. 6909 - 6912, http://dx.doi.org/10.1128/JB.00451-08
    Journal articles | 2007
    Dr Jai Tree
    Tree JJ; Ulett GC; Hobman JL; Constantinidou C; Brown NL; Kershaw C; Schembri MA; Jennings MP; McEwan AG, 2007, 'The multicopper oxidase (CueO) and cell aggregation in Escherichia coli', Environmental Microbiology, vol. 9, pp. 2110 - 2116, http://dx.doi.org/10.1111/j.1462-2920.2007.01320.x
    Journal articles | 2007
    Dr Jai Tree
    Wells TJ; Tree JJ; Ulett GC; Schembri MA, 2007, 'Autotransporter proteins: Novel targets at the bacterial cell surface', FEMS Microbiology Letters, vol. 274, pp. 163 - 172, http://dx.doi.org/10.1111/j.1574-6968.2007.00833.x
    Journal articles | 2005
    Dr Jai Tree
    Tree JJ; Kidd SP; Jennings MP; McEwan AG, 2005, 'Copper sensitivity of cueO mutants of Escherichia coli K-12 and the biochemical suppression of this phenotype', Biochemical and Biophysical Research Communications, vol. 328, pp. 1205 - 1210, http://dx.doi.org/10.1016/j.bbrc.2005.01.084
    Journal articles |
    Dr Jai Tree
    Iosub IA; Marchioretto M; Sy B; McKellar S; Nieken KJ; van Nues RW; Tree JJ; Viero G; Granneman S, 'Hfq CLASH uncovers sRNA-target interaction networks involved in adaptation to nutrient availability', , http://dx.doi.org/10.1101/481986

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