Dr Jean Bertoldo
Conjoint Senior Lecturer

Dr Jean Bertoldo

Medicine & Health
School of Clinical Medicine

Originally from Brazil, Dr Jean Bertoldo moved to Australia from Germany to join Children’s Cancer Institute in March 2022, having been appointed Team Leader in the newly established Chemical Biology and Target-Based Drug Design Group. He completed his PhD in Biochemistry at the Federal University of Santa Catarina in Brazil. He worked at both the University of Cambridge, UK, and the University of Lisbon, Portugal, developing new methods to chemically modify proteins to modulate their function. He then worked at the Institute of Molecular Medicine, University of Halle-Wittenberg, Germany, where he developed a pioneering new drug for the treatment of ovarian cancer – a covalent inhibitor of an RNA-binding protein, which is the first of its kind. At Children’s Cancer Institute, Dr Bertoldo's team is working at the interface of chemistry, drug discovery and cancer biology with the main aim of designing new drugs for the treatment of childhood cancers such as neuroblastoma, medulloblastoma and DIPG.

  • Journal articles | 2023
    Bertoldo JB; Müller S; Hüttelmaier S, 2023, 'RNA-binding proteins in cancer drug discovery', Drug Discovery Today, 28, http://dx.doi.org/10.1016/j.drudis.2023.103580
    Journal articles | 2021
    Bell JL; Malyukova A; Bertoldo JB; Summers MK; Sun Y, 2021, 'Editorial: Ubiquitin-Modifying Enzymes as Drug Targets and Biomarkers in Cancer', Frontiers in Oncology, 11, http://dx.doi.org/10.3389/fonc.2021.758983
    Journal articles | 2021
    Pessatti TB; Terenzi H; Bertoldo JB, 2021, 'Protein modifications: From chemoselective probes to novel biocatalysts', Catalysts, 11, http://dx.doi.org/10.3390/catal11121466
    Journal articles | 2020
    Martínez DG; Hüttelmaier S; Bertoldo JB, 2020, 'Unveiling Druggable Pockets by Site-Specific Protein Modification: Beyond Antibody-Drug Conjugates', Frontiers in Chemistry, 8, http://dx.doi.org/10.3389/fchem.2020.586942
    Journal articles | 2020
    Müller S; Bley N; Busch B; Glaß M; Lederer M; Misiak C; Fuchs T; Wedler A; Haase J; Bertoldo JB; Michl P; Hüttelmaier S, 2020, 'The oncofetal RNA-binding protein IGF2BP1 is a druggable, post-transcriptional super-enhancer of E2F-driven gene expression in cancer', Nucleic Acids Research, 48, pp. 8576 - 8590, http://dx.doi.org/10.1093/nar/gkaa653
    Journal articles | 2019
    Lindstedt PR; Aprile FA; Matos MJ; Perni M; Bertoldo JB; Bernardim B; Peter Q; Jiménez-Osés G; Knowles TPJ; Dobson CM; Corzana F; Vendruscolo M; Bernardes GJL, 2019, 'Enhancement of the Anti-Aggregation Activity of a Molecular Chaperone Using a Rationally Designed Post-Translational Modification', ACS Central Science, 5, pp. 1417 - 1424, http://dx.doi.org/10.1021/acscentsci.9b00467
    Journal articles | 2018
    Bertoldo JB; Terenzi H; Hüttelmaier S; Bernardes GJL, 2018, 'Posttranslational Chemical Mutagenesis: To Reveal the Role of Noncatalytic Cysteine Residues in Pathogenic Bacterial Phosphatases', Biochemistry, 57, pp. 6144 - 6152, http://dx.doi.org/10.1021/acs.biochem.8b00639
    Journal articles | 2018
    Matos MJ; Oliveira BL; Martínez-Sáez N; Guerreiro A; Cal PMSD; Bertoldo J; Maneiro M; Perkins E; Howard J; Deery MJ; Chalker JM; Corzana F; Jiménez-Osés G; Bernardes GJL, 2018, 'Chemo- and Regioselective Lysine Modification on Native Proteins', Journal of the American Chemical Society, 140, pp. 4004 - 4017, http://dx.doi.org/10.1021/jacs.7b12874
    Journal articles | 2018
    Romagny S; Bouaouiche S; Lucchi G; Ducoroy P; Bertoldo JB; Terenzi H; Bettaieb A; Plenchette S, 2018, 'S-Nitrosylation of cIAP1 switches cancer cell fate from TNFa/TNFR1-Mediated cell survival to cell death', Cancer Research, 78, pp. 1948 - 1957, http://dx.doi.org/10.1158/0008-5472.CAN-17-2078
    Journal articles | 2017
    Bertoldo J, 2017, 'The Critical Role of Non-catalytic Cysteine Residues in Proteins', CHEM, 3, pp. 525 - 526, http://dx.doi.org/10.1016/j.chempr.2017.09.013
    Journal articles | 2017
    Bertoldo JB; Rodrigues T; Dunsmore L; Aprile FA; Marques MC; Rosado LA; Boutureira O; Steinbrecher TB; Sherman W; Corzana F; Terenzi H; Bernardes GJL, 2017, 'A Water-Bridged Cysteine-Cysteine Redox Regulation Mechanism in Bacterial Protein Tyrosine Phosphatases', Chem, 3, pp. 665 - 677, http://dx.doi.org/10.1016/j.chempr.2017.07.009
    Journal articles | 2017
    Cal PMSD; Sieglitz F; Santos FMF; Parente Carvalho C; Guerreiro A; Bertoldo JB; Pischel U; Gois PMP; Bernardes GJL, 2017, 'Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells', Chemical Communications, 53, pp. 368 - 371, http://dx.doi.org/10.1039/c6cc08671c
    Journal articles | 2016
    Bertoldo JB; Terenzi H, 2016, 'Oxidation and Tyrosine Nitration Induce Structural Changes and Inhibits Plasmodium falciparum Falcipain-2 Activity In Vitro', Protein and Peptide Letters, 23, pp. 562 - 572, http://dx.doi.org/10.2174/0929866523666160411144635
    Journal articles | 2015
    Bertoldo JB; Chiaradia-Delatorre LD; Mascarello A; Leal PC; Cordeiro MNS; Nunes RJ; Sarduy ES; Rosenthal PJ; Terenzi H, 2015, 'Synthetic compounds from an in house library as inhibitors of falcipain-2 from Plasmodium falciparum', Journal of Enzyme Inhibition and Medicinal Chemistry, 30, pp. 299 - 307, http://dx.doi.org/10.3109/14756366.2014.920839
    Journal articles | 2015
    Lamotte O; Bertoldo JB; Besson-Bard A; Rosnoblet C; Aimé S; Hichami S; Terenzi H; Wendehenne D, 2015, 'Protein S-nitrosylation: specificity and identification strategies in plants', Frontiers in Chemistry, 2, http://dx.doi.org/10.3389/fchem.2014.00114
    Journal articles | 2012
    Astier J; Besson-Bard A; Lamotte O; Bertoldo J; Bourque S; Terenzi H; Wendehenne D, 2012, 'Nitric oxide inhibits the ATPase activity of the chaperone-like AAA + ATPase CDC48, a target for S-nitrosylation in cryptogein signalling in tobacco cells', Biochemical Journal, 447, pp. 249 - 260, http://dx.doi.org/10.1042/BJ20120257
    Journal articles | 2011
    Balsamo GM; Cangahuala-Inocente GC; Bertoldo JB; Terenzi H; Arisi ACM, 2011, 'Proteomic analysis of four Brazilian MON810 maize varieties and their four non-genetically-modified isogenic varieties', Journal of Agricultural and Food Chemistry, 59, pp. 11553 - 11559, http://dx.doi.org/10.1021/jf202635r
    Journal articles | 2011
    Bertoldo JB; Razzera G; Vernal J; Brod FCA; Arisi ACM; Terenzi H, 2011, 'Structural stability of Staphylococcus xylosus lipase is modulated by Zn2+ ions', Biochimica et Biophysica Acta - Proteins and Proteomics, 1814, pp. 1120 - 1126, http://dx.doi.org/10.1016/j.bbapap.2011.04.020
    Journal articles | 2010
    Brod FCA; Pelisser MR; Bertoldo JB; Vernal J; Bloch C; Terenzi H; Arisi ACM, 2010, 'Heterologous expression and purification of a heat-tolerant staphylococcus xylosus lipase', Molecular Biotechnology, 44, pp. 110 - 119, http://dx.doi.org/10.1007/s12033-009-9218-0
    Journal articles | 2010
    Brod FCA; Vernal J; Bertoldo JB; Terenzi H; Arisi ACM, 2010, 'Cloning, expression, purification, and characterization of a novel esterase from lactobacillus plantarum', Molecular Biotechnology, 44, pp. 242 - 249, http://dx.doi.org/10.1007/s12033-009-9232-2
    Journal articles | 2010
    Kolling DJ; Bertoldo JB; Brod FCA; Vernal J; Terenzi H; Arisi ACM, 2010, 'Biochemical and structural characterization of two site-directed mutants of Staphylococcus xylosus lipase', Molecular Biotechnology, 46, pp. 168 - 175, http://dx.doi.org/10.1007/s12033-010-9282-5
    Journal articles | 2009
    Bortolotto T; Bertoldo JB; Silveira FZD; Defaveri TM; Silvano J; Pich CT, 2009, 'Evaluation of the toxic and genotoxic potential of landfill leachates using bioassays', Environmental Toxicology and Pharmacology, 28, pp. 288 - 293, http://dx.doi.org/10.1016/j.etap.2009.05.007
    Journal articles | 2009
    Matiollo C; Vernal J; Ecco G; Bertoldo JB; Razzera G; de Souza EM; Pedrosa FO; Terenzi H, 2009, 'A transthyretin-related protein is functionally expressed in Herbaspirillum seropedicae', Biochemical and Biophysical Research Communications, 387, pp. 712 - 716, http://dx.doi.org/10.1016/j.bbrc.2009.07.094

My Research Supervision

John-Paul Ong. Uncovering the druggable proteome of aggressive childhood cancer for target-based drug discovery

Joshua Zhu. Mapping the tractable proteome of diffuse midline gliomas to develop targeted covalent drugs