Mr Ralf Kohler

Mr Ralf Kohler

Research Associate

NOVEMBER 2023
PhD in Biomedical Sciences / Freie Universität Berlin, Germany
Title: Bioimage analysis linking information at protein and transcriptional level in tissues

JUNE 2016
M. Eng in Applied Physics – Medical Engineering / Beuth Hochschule für Technik Berlin - Germany
Thesis: Parameter optimization for a new algorithm to improve the resolution in striped illumination microscopy via 2 photons laser excitation in tissue

SEP 2013
B. Eng in Applied Physics – Medical Engineering / Beuth Hochschule für Technik Berlin - Germany
Thesis: Determination of the modulation transfer function for PONTO II in the case of polarized neutrons

 

Medicine & Health
School of Biomedical Sciences

Motivated and dedicated multiplexed imaging specialist with a strong focus on analytical equipment maintenance and image data analysis, where the hardware-based conversion of real value measurements into extracted and functionally related model data analyses has accompanied me since my studies of Applied Physics and Medical Engineering at the "Berliner Hochschule für Technik" (B. Eng. / M. Eng., 2010-2016). As a team player who has worked in international environments, I am passionate about research, science, development and education. My technical and analytical skills in scientific project design, consulting and training students in data analysis or microscopy have resulted in high impact publications during my time at the “German Rheumatism Research Centre” in Berlin. There I worked as a microscope operator (2016-2023) and in parallel completed my PhD in Biomedical Sciences at the "Freie Universität Berlin" (2020-2023), performing image analysis at the protein and transcriptional level in tissues.

After moving to the tropics in Australia (Cairns region, 2023), I continue my research at the "University of New South Wales" (2024-present), developing an AI tool to help identify better treatments for failing hearts.

Location
School of Biomedical Sciences, Faulty of Medicine & Health Level 3, The Lowy Cancer Research Centre UNSW SYDNEY 2052
  • Journal articles | 2026
    Kroh S; Pascual-Reguant A; Manukyan A; Uecker R; Günther R; Philipsen L; Koehler R; Mex P; Landthaler M; Niesner RA; Hauser AE, 2026, 'Spatial and Phenotypic Heterogeneity of ILC Subsets in Mouse Lung Under Type 2 Inflammatory Conditions', European Journal of Immunology, 56, http://dx.doi.org/10.1002/eji.70223
    Journal articles | 2026
    Shakespeare T; Seehra RS; Flores Rodriguez N; Atuanya N; Sheard TMD; Köhler R; Bose D; Wunderley L; Woodman P; Ciani B; Jayasinghe I, 2026, 'Mapping Epidermal Growth Factor Receptor-1 Sorting Domains in Endosomes with a Calibrated Three-Dimensional Expansion Microscopy Toolkit', ACS Nano, 20, pp. 10074 - 10089, http://dx.doi.org/10.1021/acsnano.6c00277
    Journal articles | 2024
    Mertens TF; Liebheit AT; Ehl J; Koehler R; Rakhymzhan A; Woehler A; Katthaen L; Ebel G; Liublin W; Kasapi A; Triantafyllopoulou A; Schulz TJ; Niesner RA; Hauser AE, 2024, 'MarShie: a clearing protocol for 3D analysis of single cells throughout the bone marrow at subcellular resolution', NATURE COMMUNICATIONS, 15, http://dx.doi.org/10.1038/s41467-024-45827-6
    Journal articles | 2024
    Rakhymzhan A; Fiedler AF; Günther R; Domingue SR; Wooldridge L; Leben R; Cao Y; Bias A; Roodselaar J; Köhler R; Ulbricht C; Heidelin J; Andresen V; Beckers I; Haibel A; Duda G; Hauser AE; Niesner RA, 2024, 'Optimized intravital three-photon imaging of intact mouse tibia links plasma cell motility to functional states.', iScience, 27, pp. 110985, http://dx.doi.org/10.1016/j.isci.2024.110985
    Journal articles | 2023
    Mothes R; Pascual-Reguant A; Koehler R; Liebeskind J; Liebheit A; Bauherr S; Philipsen L; Dittmayer C; Laue M; von Manitius R; Elezkurtaj S; Durek P; Heinrich F; Heinz GA; Guerra GM; Obermayer B; Meinhardt J; Ihlow J; Radke J; Heppner FL; Enghard P; Stockmann H; Aschman T; Schneider J; Corman VM; Sander LE; Mashreghi M-F; Conrad T; Hocke AC; Niesner RA; Radbruch H; Hauser AE, 2023, 'Distinct tissue niches direct lung immunopathology via CCL18 and CCL21 in severe COVID-19', NATURE COMMUNICATIONS, 14, http://dx.doi.org/10.1038/s41467-023-36333-2
    Journal articles | 2021
    Dornieden T; Sattler A; Pascual-Reguant A; Ruhm AH; Thiel LG; Bergmann YS; Thole LML; Köhler R; Kühl AA; Hauser AE; Boral S; Friedersdorff F; Kotsch K, 2021, 'Signatures and Specificity of Tissue-Resident Lymphocytes Identified in Human Renal Peritumor and Tumor Tissue.', J Am Soc Nephrol, 32, pp. 2223 - 2241, http://dx.doi.org/10.1681/ASN.2020101528
    Journal articles | 2021
    Du W; Lenz D; Köhler R; Zhang E; Cendon C; Li J; Massoud M; Wachtlin J; Bodo J; Hauser AE; Radbruch A; Dong J, 2021, 'Rapid Isolation of Functional ex vivo Human Skin Tissue-Resident Memory T Lymphocytes.', Front Immunol, 12, pp. 624013, http://dx.doi.org/10.3389/fimmu.2021.624013
    Journal articles | 2021
    Pascual-Reguant A; Köhler R; Mothes R; Bauherr S; Hernández DC; Uecker R; Holzwarth K; Kotsch K; Seidl M; Philipsen L; Müller W; Romagnani C; Niesner R; Hauser AE, 2021, 'Multiplexed histology analyses for the phenotypic and spatial characterization of human innate lymphoid cells.', Nat Commun, 12, pp. 1737, http://dx.doi.org/10.1038/s41467-021-21994-8
    Journal articles | 2021
    Treimer W; Koehler R, 2021, 'Determination of the Spatial Resolution in the Case of Imaging Magnetic Fields by Polarized Neutrons', APPLIED SCIENCES-BASEL, 11, http://dx.doi.org/10.3390/app11156973
    Journal articles | 2020
    Riedel R; Addo R; Ferreira-Gomes M; Heinz GA; Heinrich F; Kummer J; Greiff V; Schulz D; Klaeden C; Cornelis R; Menzel U; Kröger S; Stervbo U; Köhler R; Haftmann C; Kühnel S; Lehmann K; Maschmeyer P; McGrath M; Naundorf S; Hahne S; Sercan-Alp Ö; Siracusa F; Stefanowski J; Weber M; Westendorf K; Zimmermann J; Hauser AE; Reddy ST; Durek P; Chang H-D; Mashreghi M-F; Radbruch A, 2020, 'Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow.', Nat Commun, 11, pp. 2570, http://dx.doi.org/10.1038/s41467-020-16464-6
    Journal articles | 2018
    Holzwarth K; Köhler R; Philipsen L; Tokoyoda K; Ladyhina V; Wählby C; Niesner RA; Hauser AE, 2018, 'Multiplexed fluorescence microscopy reveals heterogeneity among stromal cells in mouse bone marrow sections.', Cytometry A, 93, pp. 876 - 888, http://dx.doi.org/10.1002/cyto.a.23526
  • Preprints | 2026
    Köhler R; Hurley M; White E; Jayasinghe I, 2026, Structural and functional heterogeneity in cardiac RyR signalling nanodomains revealed with quantitative single molecule mapping toolkit, http://dx.doi.org/10.64898/2026.07.22.740006
    Preprints | 2025
    Shakespeare T; Seehra R; Rodriguez NF; Atuanya N; Sheard TMD; Köhler R; Bose D; Woodman P; Ciani B; Jayasinghe I, 2025, Mapping EGFR1 sorting domains in endosomes with a calibrated 3D expansion microscopy toolkit, http://dx.doi.org/10.1101/2025.10.01.678490
    Preprints | 2022
    Mothes R; Pascual-Reguant A; Koehler R; Liebeskind J; Liebheit A; Bauherr S; Dittmayer C; Laue M; von Manitius R; Elezkurtaj S; Durek P; Heinrich F; Heinz GA; Guerra GM; Obermayer B; Meinhardt J; Ihlow J; Radke J; Heppner FL; Enghard P; Stockmann H; Aschman T; Schneider J; Corman V; Sander LE; Mashreghi M-F; Conrad T; Hocke A; Niesner RA; Radbruch H; Hauser AE, 2022, Local CCL18 and CCL21 expand lung fibrovascular niches and recruit lymphocytes, leading to tertiary lymphoid structure formation in prolonged COVID-19, http://dx.doi.org/10.1101/2022.03.24.22272768
    Preprints | 2019
    Riedel R; Addo R; Ferreira-Gomes M; Heinz GA; Heinrich F; Kummer J; Greiff V; Schulz D; Klaeden C; Cornelis R; Menzel U; Kröger S; Stervbo U; Köhler R; Haftmann C; Kühnel S; Lehmann K; Maschmeyer P; McGrath M; Naundorf S; Hahne S; Sercan-Alp Ö; Siracusa F; Stefanowski J; Weber M; Westendorf K; Zimmermann J; Hauser AE; Reddy ST; Durek P; Chang H-D; Mashreghi M-F; Radbruch A, 2019, Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow, http://dx.doi.org/10.1101/825224