Embryoid Bodies
Description
Using specialised media, EBs are created spontaneously by culturing small numbers of pluripotent stem cells in suspension using a round-bottom culture ware.
These three-dimensional aggregates mimic early embryonic development and provide an in vitro system for studying differentiation into the three germ layers: ectoderm, mesoderm, and endoderm. EBs are widely used to test pluripotency, produce cells of multiple lineages and to initiate the differentiation of pluripotent stem cells into defined lineages (e.g., cerebral organoids).
Specifications
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- Pluripotent stem cell differentiation
- Suspension culture
- Specialised EB formation Media
- Short-culture length (5-10 days)
- Further differentiation into directed lineages
- High throughput capability
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- Morphological attributes
- Immunofluorescence (IF)
- Capacity to differentiate into multiple lineages
Applications
- Stem Cell Biology
- 3D Culture
- Organoids
- Cerebral Organoids Quality Control
- Differentiation
- Developmental Biology
- Multi-lineage spheroids
- Drug Screening
Instrument location
3D Culture Facility
Room 2061, Level 2
BioSciences Building (E26)
UNSW Sydney, NSW 2033
Phone: (02) 9348 8212
Email: 3DCulture@unsw.edu.au
Dr Alison Ferguson
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Phone
(02) 9348 8212 -
Email
alison.ferguson@unsw.edu.au
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