Agilent 1290 UPLC

High-efficiency UPLC instrument for versatile, routine analytical separations.
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Bioanalytical Mass Spectrometry Facility

Description

The Agilent 1290 UPLC system is comprised of binary LC pumps, a peltier cooled autosampler, column compartment with heater, diode array detector, fluorescent detector, pH and conductivity meters and a fraction collector.

Not all of these modules need to be used, and the instrument can be configured based on experimental requirements. The system can be used either as an HPLC or UHPLC.

The BMSF also has a few “generic” columns, but typically users purchase their own purpose-specific columns.

Specifications

Maximum operating pressure

  • Up to 1200 bar

Flow range

  • 0.001 to 2 mL/min

Injection range

  • 0.1 to 20 µL (expandable to 100 µL)

Column capacity

  • Up to 4 columns

Detectors

  • Diode Array (DAD) and Fluorescence

Other

  • pH and conductivity meters
  • Fraction collector available

Applications

  • Peptide mapping
  • Fractionation of complex samples and collection of fractions
  • Sample cleanup prior to mass spectrometry
  • Removal of high abundance plasma proteins

Instrument location

Bioanalytical Mass Spectrometry Facility

Room 402, Level 4 North West 
Wallace Wurth Building (C27) 
UNSW Sydney, NSW 2033

Phone: 02 9385 1717
Email: 
bmsf@unsw.edu.au

Dr Anne Poljak

Protein Chemist and Adjunct Senior Lecturer
  • Phone icon
    Phone
    02 9385 1994
  • Send email envelope icon
    Email
    a.poljak@unsw.edu.au

Dr Ling Zhong

Research Officer
  • Phone icon
    Phone
    02 9385 2717
  • Send email envelope icon
    Email
    l.zhong@unsw.edu.au

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