Thermo Orbitrap Velos
Description
The Orbitrap Velos is a hybrid instrument that incorporates both Orbitrap and linear ion trap mass analysers; both 1D and 2D nano liquid chromatography (LC) separations can be interfaced to this instrument. This platform allows the benefits of Orbitrap mass analysis to be exploited in highly sensitive experiments.
Detailed insights into analyte molecule structural characteristics can be obtained via tandem and multiple stage mass spectrometry (MS/MS and MSn) experiments, in which various methods of ion activation may be utilised. Analyses are frequently performed upon complex peptide mixtures generated via the enzymatic or chemical hydrolysis of proteins, allowing confident protein identifications to be readily made at low fmol levels. Quantitative analyses are possible via, for example, the isobaric labelling of peptides.
Specifications
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- Low flow liquid chromatography system
- Flow rate range: 50 nL/min to 50 µL/min
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- Mass Range:
- Standard: m/z 50–2,000
- Extended: m/z 200–4,000
- Resolution:
- 60,000 at m/z 400 at a scan rate of 1 Hz
- Maximum resolution: 100,000 at m/z 400
- Mass Range:
Applications
- Bottom-up proteomics
- Quantitative proteomics
- Post-Translational Modification (PTM) studies
Instrument location
Bioanalytical Mass Spectrometry Facility
Room 2012, Level 2 South
Biosciences Precinct (E26)
UNSW Sydney, NSW 2033
Phone: 02 9385 1717
Email: bmsf@unsw.edu.au
Dr Anne Poljak
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Phone
02 9385 1994 -
Email
a.poljak@unsw.edu.au
Dr Anjaneyaswamy Ravipati
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Phone
02 9385 8720 -
Email
a.ravipati@unsw.edu.au
Parent facility
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