Leica STELLARIS 8 FALCON

It supports simultaneous multicolour imaging and fluorescence lifetime imaging (FLIM)
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2025-02-07-stellaris

Description

The STELLARIS 8 FALCON is an advanced confocal microscope designed for biological research, providing multi-channel detection, flexible laser configurations, and enhanced sensitivity. It supports simultaneous multicolour imaging and fluorescence lifetime imaging (FLIM). Additionally, the integration of a far-red channel greatly improves its ability to detect and separate signals from fluorophores emitting in the red and far-red spectra.

Specifications

    • 10x Objective (HC PL APO 10x/0.40): This objective has a numerical aperture of 0.40 and a working distance of 2.56 mm.
    • 20x Objective (HC PL APO 20x/0.75): This objective has a numerical aperture of 0.75 and  a working distance of 0.62 mm.
    • 40x Water Immersion Objective (HC PL APO 40x/1.10 W CORR): With a numerical aperture of 1.10, this water immersion objective has a working distance of 0.62 mm
    • 63x Oil Immersion Objective (HC PL APO 63x/1.40 OIL): This high-resolution oil immersion objective has a numerical aperture of 1.40 and a working distance of 0.14 mm.
    • Supercontinuum White Light Laser (WLL): The Supercontinuum White Light Laser (WLL) is a pulsed laser that is tunable from 440 to 790 nm and supports the operation of up to eight laser lines simultaneously. In addition, the system has a 405 nm continuous wave (CW) diode laser.
  • The system is equipped with two Power HyD S detectors, which offer both analog and photon counting detection.

    It includes two Power HyD X detectors, specialised for ultra-sensitive applications, including fluorescence lifetime imaging (FLIM) and fluorescence correlation spectroscopy (FCS).

    There is one Power HyD R detector, providing excellent sensitivity in the near-infrared spectral range.

Applications

  • Three-Dimensional Imaging
  • Fluorescence Lifetime Imaging Microscopy (FLIM)
  • Multi-Colour Imaging

Instrument location

Katharina Gaus Light Microscopy Facility


Lowy Cancer Research Centre (C25),
Lower Ground, Room LG06,
UNSW Sydney, NSW 2033

Phone: 02 9065 5306
Email: KGlmf@unsw.edu.au

Dr Alex Macmillan

Head of KGLMF
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    alex.macmillan@unsw.edu.au

Dr Michael Carnell

Senior Lecturer
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    m.carnell@unsw.edu.au

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