Zeiss Axio Imager M2 Apotome

Enhances resolution by removing out-of-focus light through structured illumination, improving axial resolution on a widefield microscope
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Zeiss Axio Image m2 Apotome

Description

The Zeiss Axio Imager M2 upright microscope with ApoTome 2 uses the MBF Bioscience software interface (Stereo Investigator and Neurolucida) and features both a color and a monochrome camera for fluorescence applications. Its ApoTome 2 module enhances resolution by removing out-of-focus light through structured illumination, improving axial resolution on a widefield microscope with typically faster acquisition compared to a point-scan confocal microscope. Additionally, the MBF software interface provides neuronal tracing, morphometric quantification, and unbiased stereology.

Specifications

MAG N.A. CORRECTIONS IMMERSION W.D.
2.5x 0.085 EC- Plan NeoFluar Air 8.8 mm
5x 0.16 EC- Plan NeoFluar Air 18.5 mm
10x 0.45 Plan-Apochromat Air 2.1 mm
20x 0.8 Plan-Apochromat Air 0.55 mm
40x 0.95 Plan-Apochromat Air 0.25 mm
63x 1.4 Plan-Apochromat Oil 0.19 mm
    • UV: 385 / 30nm
    • V: 423 / 44nm
    • B: 469 / 38nm
    • C: 511 / 44nm
    • G: 555 / 30nm
    • Y: 590 / 27nm
    • R: 631 / 33nm
    • AxioCam 506c color digital camera
    • Hamamatsu ORCA Flash 4.0 LT Plus USB3 & CamLink: monochrome digital camera

  • NAME 

    EXCITATION 

    DICHROIC 

    EMISSION 

    FS 96 HE

    BP 370-410 nm  

    LP 420 nm 

    BP 430-470 nm 

    FS 38 

    BP 450-490 nm

    LP 495 nm

    BP 500-550 nm

    FS 43

    BP 532-558 nm

    LP 570 nm

    BP 570-640 nm

    FS 50

    BP 625-655 nm

    LP 660 nm

    BP 665-715 nm

    FS 64 HE

    BP 575-600 nm

    LP 605 nm

    BP 610-680 nm

Applications

  • Brightfield imaging
  • Widefield Fluorescence
  • Optical sectioning(ApoTome)
  • Stereology
  • Neuroanaylsis

Instrument location

Katharina Gaus Light Microscopy Facility

Level 2
Biosciences (E26)
UNSW Sydney, NSW 2033

Phone: 02 9065 5306
Email: KGlmf@unsw.edu.au

Dr Alex Macmillan

Head of KGLMF
  • Send email envelope icon
    Email
    alex.macmillan@unsw.edu.au

Dr Michael Carnell

Senior Lecturer
  • Send email envelope icon
    Email
    m.carnell@unsw.edu.au

Parent facility

Katharina Gaus Light Microscopy Facility (KGLMF)

The Katharina Gaus Light Microscopy Facility, formerly known as the Biomedical Imaging Facility (BMIF), is a dedicated team of scientists committed to advancing research through comprehensive support and training in microscopy.

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