Zeiss LSM 880

The system supports fluorescence lifetime imaging (FLIM), correlation spectroscopy (FCS), energy transfer (FRET), and anisotropy measurements,
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Zeiss 880

Description

The Zeiss LSM 880 is an advanced inverted laser scanning confocal microscope designed for high-resolution imaging with improved sensitivity and versatility. It features a GaAsP detector, multi-photon capabilities for imaging thick specimens, and an Airyscan module that enhances resolution and signal-to-noise ratio. The system supports fluorescence lifetime imaging (FLIM), correlation spectroscopy (FCS), energy transfer (FRET), and anisotropy measurements, making it suitable for a range of complex imaging applications.

Specifications

  • MAG

    N.A

    CORRECTIONS

    IMMERSION

    W.D

    MISC.

    10x

    0.45

    Plan-Apochromat

    Air

    2mm

     

    20x

    0.8

    Plan-Apochromat

    Air

    0.55mm

     

    32x

    0.85

    C-Achroplan

    Water

    1.1mm

    VIS-IR

    40x

    1.3

    Plan-Apochromat

    Oil

    0.21mm

    DIC

    40x

    1.1

    C-Apochromat

    Water

    0.62mm

    UV-VIS-IR DIC

    63x

    1.4

    Plan-Apochromat

    Oil

    .19mm

    DIC

  • LASERS

    WAVELENGTHS

    Diode 405

    405nm

    Argon ion

    458nm, 488nm, 514nm

    DPSS 561

    561nm

    HeNe 594

    594nm

    HeBe 633

    633nm

    Mai Tai Insight DeepSee

    Tunnable: 680 - 1300nm

    Pulse width <120fs

    Repetition rate 80 MHz

     

  • Widefield (Eyepiece Only)

    NAME

    EXCITATION

    DICHROIC

    EMISSION

    FSet 38

    BP 450 - 490

    LP 495

    BP 500 - 550

    FSet 43

    BP 533 - 558

    LP 570

    BP 570 - 640

    FSet 49

    365

    LP 395

    BP 420 - 470
    • BP 420-475 + BP 500 - 545
    • BP 500-545 + LP 575
    • BP 575-615 + LP 655
    • BP 420-480 + BP 495-620
    • BP 465-505 + LP 525
    • BP 420-480 + LP 600
    • Tunnable wevelength selection for all 3 fluorescence/reflective detectors in the range of 400nm - 750nm
    • SP485 / 500-550
    • 500-550/270-610

    *Filter holder also contains a position for one of two shortpass IR blocking filters. either SP690 or SP 760
    • Spectral detector 32 channels GaAsP PMT plus 2 channels Multi-Alkali PMT
    • BiG.2 Type A detector module consisting of 2x GaAsP detectors 
    • Airyscan GaAsP array

     

Funding agency

  • Funded by Cancer Institute NSW

Applications

  • Confocal
  • Spectral Unmixing
  • Super-Resolution (AiryScan)
  • FLIM
  • Multi-photon
  • Live cell imaging
  • Second Harmonic Generation
  • FRAP
  • FCS/FCCS

Related instruments

Instrument location

Katharina Gaus Light Microscopy Facility


Lowy Cancer Research Centre (C25),
Lower Ground, Room LG06,
UNSW Sydney, NSW 2033

Phone: 02 9065 5306
Email: KGlmf@unsw.edu.au

Dr Alex Macmillan

Head of KGLMF
  • Send email envelope icon
    Email
    alex.macmillan@unsw.edu.au

Dr Michael Carnell

Senior Lecturer
  • Send email envelope icon
    Email
    m.carnell@unsw.edu.au

Parent facility

Katharina Gaus Light Microscopy Facility (KGLMF)

The Katharina Gaus Light Microscopy Facility, formerly known as the Biomedical Imaging Facility (BMIF), is a dedicated team of scientists committed to advancing research through comprehensive support and training in microscopy.

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