Dr Anne Marie Galea
Senior Lecturer

Dr Anne Marie Galea

Science
Biotech & Biomolecular Science

Research Contribution

My work has primarily been focused on investigating the mechanism of action of the DNA-damaging anti-tumour drug, cisplatin, and related compounds. My Honours and PhD projects explored anti-tumour drug-DNA interactions in a reconstituted chromatin system and the gene expression response of human cells to drug treatments.

When the influence of chromatin structure on cisplatin DNA damage was investigated in a reconstituted nucleosome system, the major significant finding was that the preferred site of cisplatin DNA binding was in the linker region of nucleosomal DNA. Three different cisplatin analogues were investigated using the same reconstituted nucleosome system and were also found to target the linker region of the nucleosome. The finding that the presence of nucleosome core proteins inhibits the reaction of cisplatin (and analogues) with DNA in chromatin has several important implications for the design of anti-tumour drugs based on cisplatin. The gene expression work was conducted using human cells and microarray technology. The purpose of this study was to clarify the molecular events that are important to the anti-tumour activity of cisplatin, using gene expression profiling techniques. Human foreskin fibroblast cells were treated with cisplatin or its clinically inactive isomer, transplatin. Dual-fluor microarray experiments comparing treated and untreated cells were then performed.

Drug-induced gene expression profiles constructed from the resulting data consistently described a subset of genes that are significantly regulated in response to drug treatment. In addition, several genes were identified that display different expression responses in cisplatin- and transplatin-treated cells. The discovery of expression traits unique to compounds with clinically effective anti-tumour properties, like cisplatin, could enable the identification of new drug targets and ultimately permit the design and development of improved cancer chemotherapeutic agents with less harmful side effects. Alternative functional studies are now being considered in order to investigate the potential role of such genes in mediating a cytotoxic response.

Professional Experience

  • 2000-2005: PhD student, casual tutor and demonstrator
  • 2006-2010: Associate Lecturer, School of Biotechnology and Biomolecular Sciences, UNSW
  • 2011-2019: Lecturer, School of Biotechnology and Biomolecular Sciences, UNSW
  • 2018-current: Deputy Director of Teaching, School of Biotechnology and Biomolecular Sciences, UNSW
  • 2019-current: Senior Lecturer, School of Biotechnology and Biomolecular Sciences, UNSW

Honours & Awards

  • Australian Postgraduate Award
  • 2012: UNSW Faculty of Science Award for Excellence in Teaching
  • 2018: UNSW Vice-Chancellor's Award for Excellence in Teaching
Phone
(+61 2) 9385 8156
Location
Office 220B, Level 2 West Biological Sciences Building D26

Publications

  • Journal articles | 2021
    Dr Anne Marie Galea
    Kava HW; Leung WY; Galea AM; Murray V, 2021, 'The DNA binding properties of 9-aminoacridine carboxamide Pt complexes', Bioorganic and Medicinal Chemistry, vol. 40, http://dx.doi.org/10.1016/j.bmc.2021.116191
    Journal articles | 2017
    Dr Anne Marie Galea
    Delaney SK; Mills J; Galea A; LeBard RJ; Wilson J; Gibson K; Kornfeld G; Ashraf G, 2017, 'Analysis of Alternative Strategies for the Teaching of Difficult Threshold Concepts in Large Undergraduate Medicine and Science Classes', Medical Science Educator, vol. 27, pp. 673 - 684, http://dx.doi.org/10.1007/s40670-017-0453-x
    Journal articles | 2014
    Dr Anne Marie Galea
    Murray V; Chen JK; Galea AM, 2014, 'The anti-tumor drug bleomycin preferentially cleaves at the transcription start sites of actively transcribed genes in human cells', Cellular and Molecular Life Sciences, vol. 71, pp. 1505 - 1512, http://dx.doi.org/10.1007/s00018-013-1456-4
    Journal articles | 2014
    Dr Anne Marie Galea
    Kava HW; Galea AM; Md. Jamil F; Feng Y; Murray V, 2014, 'Characterising the atypical 5′-CG DNA sequence specificity of 9-aminoacridine carboxamide Pt complexes', Journal of Biological Inorganic Chemistry, vol. 19, pp. 997 - 1007, http://dx.doi.org/10.1007/s00775-014-1144-3
    Journal articles | 2014
    Dr Anne Marie Galea
    Murray V; Chen JK; Galea AM, 2014, 'The potential of acridine carboxamide Pt complexes as anti-cancer agents: A review', Anti-Cancer Agents in Medicinal Chemistry, vol. 14, pp. 695 - 705, http://dx.doi.org/10.2174/18715206113136660361
    Journal articles | 2014
    Dr Anne Marie Galea
    Murray V; Chen JK; Galea AM, 2014, 'Enhanced DNA repair of bleomycin-induced 3'-phosphoglycolate termini at the transcription start sites of actively transcribed genes in human cells', Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, vol. 769, pp. 93 - 99, http://dx.doi.org/10.1016/j.mrfmmm.2014.06.006
    Journal articles | 2013
    Dr Anne Marie Galea
    Nguyen HT; Galea AM; Murray V, 2013, 'The interaction of cisplatin with a human telomeric DNA sequence containing seventeen tandem repeats', Bioorganic and Medicinal Chemistry Letters, vol. 23, pp. 1041 - 1045, http://dx.doi.org/10.1016/j.bmcl.2012.12.021
    Journal articles | 2012
    Dr Anne Marie Galea
    Yang H; Sytnyk V; Crossley M; Galea AM, 2012, 'Controlling the size of lipid droplets: Lipid and protein factors', Current Opinion in Cell Biology, vol. 24, pp. 509 - 516, http://dx.doi.org/10.1016/j.ceb.2012.05.012
    Journal articles | 2012
    Dr Anne Marie Galea
    Murray V; Galea AM, 2012, 'Gene expression profiling of the cancer chemotherapeutic agent, Cisplatin, compared with its ineffective isomer, Transplatin.', Journal of Computer Science and Systems Biology, vol. 5, pp. 37, http://dx.doi.org/10.4172/0974-7230.S1.02
    Journal articles | 2010
    Dr Anne Marie Galea
    Brown AJ; Galea AM, 2010, 'Cholesterol as an evolutionary response to living with oxygen.', Evolution, vol. 64, pp. 2179 - 2183, http://dx.doi.org/10.1111/j.1558-5646.2010.01011.x
    Journal articles | 2009
    Dr Anne Marie Galea
    Galea AM; Brown AJ, 2009, 'Special relationship between sterols and oxygen: Were sterols an adaptation to aerobic life?', Free Radical Biology and Medicine, vol. 47, pp. 880 - 889, http://dx.doi.org/10.1016/j.freeradbiomed.2009.06.027
    Journal articles | 2008
    Dr Anne Marie Galea
    Galea AM; Murray VM, 2008, 'The anti-tumour agent, Cisplatin, and its clinically ineffective isomer, Transplatin, produce unique gene expression profiles in human cells', Cancer Informatics, vol. 6, pp. 315 - 355
    Journal articles | 2002
    Dr Anne Marie Galea
    Galea AM; Murray V, 2002, 'The interaction of cisplatin and analogues with DNA in reconstituted chromatin', Biochimica et Biophysica Acta - Gene Structure and Expression, vol. 1579, pp. 142 - 152, http://dx.doi.org/10.1016/S0167-4781(02)00535-3
    Journal articles | 2002
    Dr Anne Marie Galea
    Galea AM; Murray VM, 2002, 'Cisplatin preferentially targets the linker region of the nucleosome', Biochim Biophys ACTA, vol. 1579, pp. 142 - 152
  • Conference Papers | 2009
    Dr Anne Marie Galea
    Murray V; Galea AM, 2009, 'The Unique Gene Expression Profile of the Anti-tumour Agent, Cisplatin, Compared with its Clinically Ineffective Isomer, Transplatin.', in Proceedings of the 2nd International Conference on Biomedical and Pharmaceutical Engineering, IEEE, Singapore, presented at 2nd International Conference on Biomedical and Pharmaceutical Engineering, Singapore, 02 December 2009 - 04 December 2009, http://dx.doi.org/10.1109/ICBPE.2009.5384103
    Theses / Dissertations | 2006
    Dr Anne Marie Galea
    Galea AM, 2006, Investigating the mechanism of action of the chemotherapeutic agent, cisplatin: drug-DNA interactions in reconstituted chromatin and human gene expression profiling.
    Conference Papers | 2002
    Dr Anne Marie Galea
    Galea AM; Murray VM, 2002, 'Investigating the effect of cisplatin on human gene expression using cDNA microarrays', in Takayama K (ed.), School of Biotechnology and Biomolecular Sciences 1st Annual Symposium, School of Biotechnology and Biomolecular Sciences 1st Annual Symposium, Sydney, presented at School of Biotechnology and Biomolecular Sciences 1st Annual Symposium, Sydney, 08 November 2002
    Conference Papers | 2002
    Dr Anne Marie Galea
    Galea AM; Murray VM, 2002, 'The Interaction of cisplatin with DNA in reconstituted chromatin', in Genome Conference 2001: 22nd Annual Conference on the Organisation and Expression of the Genome, Genome Conference 2001: 22nd Annual Conference on the Organisation and Expression of the Genome, Lorne, Victoria, pp. 44 - 44, presented at Genome Conference 2001: 22nd Annual Conference on the Organisation and Expression of the Genome, Lorne, Victoria, 17 February 2002 - 21 February 2002
    Conference Papers | 2002
    Dr Anne Marie Galea
    Galea AM; Murray VM, 2002, 'The interaction of anti-tumour agents with nucleosomal core DNA in reconstituted chromatin', in ComBio Conference, ComBio Conference, Sydney, presented at ComBio Conference, Sydney
    Conference Papers | 2002
    Dr Anne Marie Galea
    Galea AM; Murray VM, 2002, 'Gene expression profiling of human tumour cells using cDNA microarrays: a model test system for anti-tumour drug development', in 2nd Australian Microarray Meeting, 2nd Australian Microarray Meeting, Sth Stradbroke Island, Australia, presented at 2nd Australian Microarray Meeting, Sth Stradbroke Island, Australia

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