Professor John Pimanda
Professor

Professor John Pimanda

Medicine & Health
School of Medical Sciences

We are a molecular and cell biology laboratory with a translational research focus. We study healthy blood stem cells and leukaemic cells. Our investigations are at genome-scale and in single cells. We use this knowledge to improve our biological understanding and management of blood stem disorders such as myelodysplasia and acute myeloid leukaemia. 

Our research interest lies in identifying critical components of the haematopoietic transcriptional network and how it governs stemness. How this network is perturbed in mutant blood stem cells and its impact on clonal dominance and maintenance of leukaemia is a focus. The role of stromal cells in the development of blood stem cells and maintenance of leukaemic cells is also an area of research interest. 

Phone
02 93851003
Location
University-Lowy Cancer Research Centre, UNSW,Sydney Hospital- Medical Professorial Unit, Edmund Blacket Building, The Prince of Wales Hospital, Randwick
  • Journal articles | 2022
    Chandrakanthan V; Rorimpandey P; Zanini F; Chacon D; Olivier J; Joshi S; Kang YC; Knezevic K; Huang Y; Qiao Q; Oliver RA; Unnikrishnan A; Carter DR; Lee B; Brownlee C; Power C; Brink R; Mendez-Ferrer S; Enikolopov G; Walsh W; Göttgens B; Taoudi S; Beck D; Pimanda JE, 2022, 'Mesoderm-derived PDGFRA+ cells regulate the emergence of hematopoietic stem cells in the dorsal aorta', Nature Cell Biology, vol. 24, pp. 1211 - 1225, http://dx.doi.org/10.1038/s41556-022-00955-3
    Journal articles | 2022
    Liu YC; Kwon J; Fabiani E; Xiao Z; Liu YV; Follo MY; Liu J; Huang H; Gao C; Liu J; Falconi G; Valentini L; Gurnari C; Finelli C; Cocco L; Liu JH; Jones AI; Yang J; Yang H; Thoms JAI; Unnikrishnan A; Pimanda JE; Pan R; Bassal MA; Voso MT; Tenen DG; Chai L, 2022, 'Demethylation and Up-Regulation of an Oncogene after Hypomethylating Therapy', New England Journal of Medicine, vol. 386, pp. 1998 - 2010, http://dx.doi.org/10.1056/NEJMoa2119771
    Journal articles | 2022
    Putri GH; Anders S; Pyl PT; Pimanda JE; Zanini F, 2022, 'Analysing high-throughput sequencing data in Python with HTSeq 2.0', Bioinformatics, vol. 38, pp. 2943 - 2945, http://dx.doi.org/10.1093/bioinformatics/btac166
    Journal articles | 2021
    Chandrakanthan V; Rorimpandey P; Zanini F; Chacon D; Kang YC; Knezevic K; Huang Y; Qiao Q; Oliver R; Unnikrishnan A; Carter D; Lee B; Brownlee C; Power C; Mendez-Ferrer S; Enikolopov G; Walsh W; Göttgens B; Taoudi S; Beck D; Pimanda J, 2021, 'Mesoderm-Derived PDGFRA+ Cells Regulate the Emergence of Hematopoietic Stem Cells in the Dorsal Aorta', , http://dx.doi.org/10.1101/2021.08.08.455592
    Journal articles | 2016
    Botezatu L; Michel LC; Makishima H; Schroeder T; Germing U; Haas R; Van der Reijden B; Marneth AE; Bergevoet SM; Jansen JH; Przychodzen B; Wlodarski M; Niemeyer C; Platzbecker U; Ehninger G; Unnikrishnan A; Beck D; Pimanda J; Hellström-Lindberg E; Malcovati L; Boultwood J; Pellagatti A; Papaemmanuil E; Le Coutre P; Kaeda J; Opalka B; Möröy T; Dührsen U; Maciejewski J; Khandanpour C, 2016, 'GFI136N as a therapeutic and prognostic marker for myelodysplastic syndrome', Experimental Hematology, vol. 44, pp. 590 - 595.e1, http://dx.doi.org/10.1016/j.exphem.2016.04.001
    Journal articles | 2016
    Hesson LB; Ng B; Zarzour P; Srivastava S; Kwok CT; Packham D; Nunez AC; Beck D; Ryan R; Dower A; Ford CE; Pimanda JE; Sloane MA; Hawkins NJ; Bourke MJ; Wong JWH; Ward RL, 2016, 'Integrated genetic, epigenetic, and transcriptional profiling identifies molecular pathways in the development of laterally spreading tumors', Molecular Cancer Research, vol. 14, pp. 1217 - 1228, http://dx.doi.org/10.1158/1541-7786.MCR-16-0175
    Journal articles | 2016
    Huang Y; Thoms JAI; Tursky ML; Knezevic K; Beck D; Chandrakanthan V; Suryani S; Olivier J; Boulton A; Glaros EN; Thomas SR; Lock RB; MacKenzie KL; Bushweller JH; Wong JWH; Pimanda JE, 2016, 'MAPK/ERK2 phosphorylates ERG at serine 283 in leukemic cells and promotes stem cell signatures and cell proliferation', Leukemia, vol. 30, pp. 1552 - 1561, http://dx.doi.org/10.1038/leu.2016.55
    Journal articles | 2016
    Khanna A; Pimanda JE, 2016, 'Clinical significance of cancerous inhibitor of protein phosphatase 2A in human cancers', International Journal of Cancer, vol. 138, pp. 525 - 532, http://dx.doi.org/10.1002/ijc.29431
    Journal articles | 2016
    Richmond J; Robbins A; Evans K; Beck D; Kurmasheva RT; Billups CA; Carol H; Heatley S; Sutton R; Marshall GM; White D; Pimanda J; Houghton PJ; Smith MA; Lock RB, 2016, 'Acute sensitivity of Ph-like acute lymphoblastic leukemia to the SMAC-mimetic birinapant', Cancer Research, vol. 76, pp. 4579 - 4591, http://dx.doi.org/10.1158/0008-5472.CAN-16-0523
    Journal articles | 2016
    Shields BJ; Jackson JT; Metcalf D; Shi W; Huang Q; Garnham AL; Glaser SP; Beck D; Pimanda JE; Bogue CW; Smyth GK; Alexander WS; McCormack MP, 2016, 'Acute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a', Genes and Development, vol. 30, pp. 78 - 91, http://dx.doi.org/10.1101/gad.268425.115
    Journal articles | 2015
    Giotopoulos G; van der Weyden L; Osaki H; Rust AG; Gallipoli P; Meduri E; Horton SJ; Chan WI; Foster D; Prinjha RK; Pimanda JE; Tenen DG; Vassiliou GS; Koschmieder S; Adams DJ; Huntly BJP, 2015, 'A novel mouse model identifies cooperating mutations and therapeutic targets critical for chronic myeloid leukemia progression', Journal of Experimental Medicine, vol. 212, pp. 1551 - 1569, http://dx.doi.org/10.1084/jem.20141661
    Journal articles | 2015
    Jing RBAB, 2015, 'Opposing regulation of BIM and BCL2 controls glucocorticoid-induced apoptosis of pediatric acute lymphoblastic leukemia cells', Blood, vol. 125, pp. 273 - 283, http://dx.doi.org/10.1182/blood-2014-05-576470
    Journal articles | 2015
    Kartalaei PS; Yamada-Inagawa T; Vink CS; de Pater E; van der Linden R; Marks-Bluth J; van der Sloot A; van den Hout M; Yokomizo T; Lucila van Schaick-Solernó M; Delwel R; Pimanda JE; van IJcken WFJ; Dzierzak E, 2015, 'Whole-transcriptome analysis of endothelial to hematopoietic stem cell transition reveals a requirement for Gpr56 in HSC generation', Journal of Experimental Medicine, vol. 212, pp. 93 - 106, http://dx.doi.org/10.1084/jem.20140767
    Journal articles | 2015
    Khanna A; Rane JK; Kivinummi KK; Urbanucci A; Helenius MA; Tolonen TT; Saramäki OR; Latonen L; Manni V; Pimanda JE; Maitland NJ; Westermarck J; Visakorpi T, 2015, 'CIP2A is a candidate therapeutic target in clinically challenging prostate cancer cell populations', Oncotarget, vol. 6, pp. 19661 - 19670, http://dx.doi.org/10.18632/oncotarget.3875
    Journal articles | 2015
    Marks-Bluth J; Khanna A; Chandrakanthan V; Thoms J; Bee T; Eich C; Kang YC; Knezevic K; Qiao Q; Fitch S; Oxburgh L; Ottersbach K; Dzierzak E; de Bruijn MFTR; Pimanda JE, 2015, 'SMAD1 and SMAD5 expression is coordinately regulated by FLI1 and GATA2 during endothelial development', Molecular and Cellular Biology, vol. 35, pp. 2165 - 2172, http://dx.doi.org/10.1128/MCB.00239-15
    Journal articles | 2015
    Nasrallah R; Knezevic K; Thai T; Thomas SR; Göttgens B; Lacaud G; Kouskoff V; Pimanda JE, 2015, 'Endoglin potentiates nitric oxide synthesis to enhance definitive hematopoiesis', Biology Open, vol. 4, pp. 819 - 829, http://dx.doi.org/10.1242/bio.011494
    Journal articles | 2015
    Rao TN; Marks-Bluth J; Sullivan J; Gupta MK; Chandrakanthan V; Fitch SR; Ottersbach K; Jang YC; Piao X; Kulkarni RN; Serwold T; Pimanda JE; Wagers AJ, 2015, 'High-level Gpr56 expression is dispensable for the maintenance and function of hematopoietic stem and progenitor cells in mice', Stem Cell Research, vol. 14, pp. 307 - 322, http://dx.doi.org/10.1016/j.scr.2015.02.001
    Journal articles | 2015
    Tursky ML; Beck D; Thoms JAI; Huang Y; Kumari A; Unnikrishnan A; Knezevic K; Evans K; Richards LA; Lee E; Morris J; Goldberg L; Izraeli S; Wong JWH; Olivier J; Lock RB; MacKenzie KL; Pimanda JE, 2015, 'Overexpression of ERG in cord blood progenitors promotes expansion and recapitulates molecular signatures of high ERG leukemias', Leukemia, vol. 29, pp. 819 - 827, http://dx.doi.org/10.1038/leu.2014.299
    Journal articles | 2014
    Chacon D; Beck D; Perera D; Wong JWH; Pimanda JE, 2014, 'BloodChIP: A database of comparative genome-wide transcription factor binding profiles in human blood cells', Nucleic Acids Research, vol. 42, http://dx.doi.org/10.1093/nar/gkt1036
    Journal articles | 2014
    Ju YSE; Alexandrov LB; Gerstung M; Martincorena I; Nik-Zainal S; Ramakrishna M; Davies HR; Papaemmanuil E; Gundem G; Shlien A; Bolli N; Behjati S; Tarpey PS; Nangalia J; Massie CE; Butler AP; Teague JW; Vassiliou GS; Green AR; Du MQ; Unnikrishnan A; Pimanda JE; Teh BTE; Munshi N; Greaves M; Vyas P; El-Naggar AK; Santarius T; Collins VP; Grundy R; Taylor JA; Hayes DN; Malkin D; Foster CS; Warren AY; Whitaker HC; Brewer D; Eeles R; Cooper C; Neal D; Visakorpi T; Isaacs WB; Bova GS; Flanagan AM; Futreal PA; Lynch AG; Chinnery PF; McDermott U; Stratton MR; Campbell PJ, 2014, 'Origins and functional consequences of somatic mitochondrial DNA mutations in human cancer', eLife, vol. 3, http://dx.doi.org/10.7554/eLife.02935
    Journal articles | 2014
    Perera D; Chacon D; Thoms JA; Poulos RC; Shlien A; Beck D; Campbell PJ; Pimanda JE; Wong JW, 2014, 'Oncocis: Annotation of cis-regulatory mutations in cancer', Genome Biology, vol. 15, http://dx.doi.org/10.1186/s13059-014-0485-0
    Journal articles | 2014
    Sloane MA; Wong JWH; Perera D; Nunez AC; Pimanda JE; Hawkins NJ; Sieber OM; Bourke MJ; Hesson LB; Ward RL, 2014, 'Epigenetic inactivation of the candidate tumor suppressor USP44 is a frequent and early event in colorectal neoplasia', Epigenetics, vol. 9, pp. 1092 - 1100, http://dx.doi.org/10.4161/epi.29222
    Journal articles | 2013
    Beck D; Thoms JAI; Perera D; Schütte J; Unnikrishnan A; Knezevic K; Kinston SJ; Wilson NK; O'Brien TA; Göttgens B; Wong JWH; Pimanda JE, 2013, 'Genome-wide analysis of transcriptional regulators in human HSPCs reveals a densely interconnected network of coding and noncoding genes', Blood, vol. 122, pp. e12 - e22, http://dx.doi.org/10.1182/blood-2013-03-490425
    Journal articles | 2013
    Goldberg L; Tijssen MR; Birger Y; Hannah RL; Kinston SJ; Schütte J; Beck D; Knezevic K; Schiby G; Jacob-Hirsch J; Biran A; Kloog Y; Marcucci G; Bloomfield CD; Aplan PD; Pimanda JE; Göttgens B; Izraeli S, 2013, 'Genome-scale expression and transcription factor binding profiles reveal therapeutic targets in transgenic ERG myeloid leukemia', Blood, vol. 122, pp. 2694 - 2703, http://dx.doi.org/10.1182/blood-2013-01-477133
    Journal articles | 2013
    Khanna A; Kauko O, Bockelman C, Laine A, Schreck I, Partanen JI, Szwajda A, Bormann S, Bilgen T, Helenius M, Pokharel YR, Pimanda J, Russel MR, Haglund C, Cole KA, Klefstrom J, Aittokallio T, Weiss C, Ristimaki A, Visakorpi T, Westermarck J. , 2013, 'Chk1 targeting reactivates PP2A tumor suppressor activity in cancer cells.', Cancer Research, vol. 73, pp. 6757 - 6769, http://dx.doi.org/10.1158/0008-5472.CAN-13-1002
    Journal articles | 2011
    Chong J; Chandrakanthan V; Xaymardan M; Asli NS; Li J; Ahmed I; Heffernan C; Menon M; Scarlett C; Rashidianfar A; Biben C; Zoellner H; Colvin EK; Pimanda JE; Biankin AV; Zhou B; Pu WT; Prall OWJ; Harvey RP, 2011, 'Adult cardiac-resident MSC-like stem cells with a proepicardial origin', Cell Stem Cell, vol. 9, pp. 527 - 540, http://dx.doi.org/10.1016/j.stem.2011.10.002
  • Preprints | 2020
    Liu Y-C; Fabiani E; Kwon J; Gao C; Falconi G; Valentini L; Gurnari C; Liu YV; Jones AI; Yang J; Yang H; Thoms JAI; Unnikrishnan A; Pimanda JE; Pan R; Voso MT; Tenen DG; Chai L, 2020, Demethylation and upregulation of an oncogene post hypomethylating treatment, http://dx.doi.org/10.1101/2020.07.21.20157776
    Preprints | 2020
    Yang J; Gao C; Liu M; Chen Z; Liu Y-C; Kwon J; Qi J; Tian X; Stein A; Liu Y; Kong NR; Wu Y; Yin S; Xi JJ; Luo H; Silberstein LE; Thoms JAI; Unnikrishnan A; Pimanda JE; Tenen DG; Chai L, 2020, Induction of a SALL4-dependency for targeted cancer therapy, http://dx.doi.org/10.1101/2020.07.10.197434