Dr Joyce Chiu

Dr Joyce Chiu

Lecturer
Medicine & Health
School of Clinical Medicine

Bio: Dr Joyce Chiu has a long-standing interest in the molecular control of protein functions by redox switches. She developed proteomic methods for detection of redox modifications in proteins and pioneered the discovery of force-redox regulation of integrin de-adhesion in clot formation and inflammation. Her expertise in redox enzymes also contributed to the discovery of a new class of anti-thrombotic compounds and is an inventor of a bioactive factor for bone formation. Joyce's work has been published in the top-tier biomedical journals, including eLife, Blood, and Circulation Research. Her contributions to her research field led to the award of the Helen and Robert Ellis Postdoctoral Fellowship (2017-2019) and the Professor Tony Basten Postdoctoral Fellowship (2018) from the Sydney Medical School Foundation. Joyce received the Outstanding Young Scientist Award from the Joint Symposium of the Asian Pacific Society of Thrombosis and Haemostasis and Japanese Society of Thrombosis and Haemostasis in 2021 and an Oral Communication Award from the 15th World Congress on Inflammation in 2022. Joyce was invited to speak at the Gordon Research Seminar for Phagocytes and the NSW Cardiovascular Research Network Rising Stars Seminar Series in 2023. Her research contributions are internationally recognised as evident by her nomination to full membership of the prestigious scientific research honour society, Sigma XI, in 2024. Joyce is currently an associate editor for the journal, Bio-protocol, and a reviewer for peer-reviewed journals, including Nature Communications, Biochemical Pharmacology, and Journal of Cellular and Molecular Medicine.

Research interest: Cardiovascular disease is the leading cause of death worldwide, accounting for >30% of all deaths per year. Thrombosis, or clot formation in the arteries, obstructs blood flow and underlies the pathology of heart attack, stroke, and deep vein thrombosis, to name a few. Using cross-disciplinary approaches including proteomics, microfluidics, animal models, and computational biology, Joyce discovered novel force-redox mechanisms in clot formation and immune-cell migration. Her current research focuses on (i) the redox control of G protein-coupled receptors in platelets, (ii) the mechanics of immune cell migration in blood flow, and (iii) the development of novel inhibitors against redox enzymes. 

Phone
02 9113 2058
Location
Research and Education Centre St George Hospital Campus Belgrave Street Kogarah, NSW
  • Books | 2019
    , 2019, Functional Disulphide Bonds, Hogg P, (ed.), Springer New York, http://dx.doi.org/10.1007/978-1-4939-9187-7
  • Book Chapters | 2019
    Chiu J, 2019, 'Quantification of the redox state of protein disulphide bonds', in Methods in Molecular Biology, pp. 45 - 63, http://dx.doi.org/10.1007/978-1-4939-9187-7_4
  • Journal articles | 2024
    Kong YX; Chiu J; Passam FH, 2024, '“Sticki-ER”: Functions of the Platelet Endoplasmic Reticulum', Antioxidants and Redox Signaling, 41, pp. 637 - 660, http://dx.doi.org/10.1089/ars.2024.0566
    Journal articles | 2024
    Li Y; Xu X; Wang HJ; Chen YC; Chen Y; Chiu J; Li L; Wang L; Wang J; Tang Z; Ren L; Li H; Wang X; Jin S; Wu Y; Huang M; Ju LA; Fang C, 2024, 'Endoplasmic Reticulum Protein 72 Regulates Integrin Mac-1 Activity to Influence Neutrophil Recruitment', Arteriosclerosis, Thrombosis, and Vascular Biology, 44, pp. E82 - E98, http://dx.doi.org/10.1161/ATVBAHA.123.319771
    Journal articles | 2024
    Lv K; Chen S; Xu X; Chiu J; Wang HJ; Han Y; Yang X; Bowley SR; Wang H; Tang Z; Tang N; Yang A; Yang S; Wang J; Jin S; Wu Y; Schmaier AH; Ju LA; Hogg PJ; Fang C, 2024, 'Protein disulfide isomerase cleaves allosteric disulfides in histidine-rich glycoprotein to regulate thrombosis', Nature Communications, 15, pp. 3129, http://dx.doi.org/10.1038/s41467-024-47493-0
    Journal articles | 2023
    Dupuy A; Aponte-Santamaria C; Yeheskel A; Hortle E; Oehlers SH; Grater F; Hogg PJ; Passam FH; Chiu J, 2023, 'Mechano-Redox Control of Mac-1 De-Adhesion by PDI Promotes Directional Movement under Flow', Circulation Research, 132, pp. E151 - E168, http://dx.doi.org/10.1161/CIRCRESAHA.122.321926
    Journal articles | 2023
    Kutzki F; Butera D; Lay AJ; Maag D; Chiu J; Woon HG; Kubař T; Elstner M; Aponte-Santamaría C; Hogg PJ; Gräter F, 2023, 'Disulfide bond reduction and exchange in C4 domain of von Willebrand factor undermines platelet binding', Journal of Thrombosis and Haemostasis, 21, pp. 2089 - 2100, http://dx.doi.org/10.1016/j.jtha.2023.03.039
    Journal articles | 2023
    Lay AJ; Dupuy A; Hagimola L; Tieng J; Larance M; Zhang Y; Yang J; Kong Y; Chiu J; Gray E; Qin Z; Schmidt D; Maclean J; Hofma B; Ellis M; Kalev-Zylinska M; Argon Y; Jackson SP; Hogg P; Passam FH, 2023, 'Endoplasmic reticulum protein 5 attenuates platelet endoplasmic reticulum stress and secretion in a mouse model', Blood Advances, 7, pp. 1650 - 1665, http://dx.doi.org/10.1182/bloodadvances.2022008457
    Journal articles | 2023
    Yang M; Chiu J; Scartelli C; Ponzar N; Patel S; Patel A; Ferreira RB; Keyes RF; Carroll KS; Pozzi N; Hogg PJ; Smith BC; Flaumenhaft R, 2023, 'Sulfenylation links oxidative stress to protein disulfide isomerase oxidase activity and thrombus formation', Journal of Thrombosis and Haemostasis, 21, pp. 2137 - 2150, http://dx.doi.org/10.1016/j.jtha.2023.03.034
    Journal articles | 2022
    Dupuy A; Ju LA; Chiu J; Passam FH, 2022, 'Mechano-Redox Control of Integrins in Thromboinflammation', Antioxidants and Redox Signaling, 37, pp. 1072 - 1093, http://dx.doi.org/10.1089/ars.2021.0265
    Journal articles | 2021
    Chiu J; Uaprasert N; Eriksson O; Lin L; Guo S; Hogg P; Flaumenhaft R, 2021, 'PDI Cleavage of Disulfide Bonds within the TP Receptor Inhibits Signaling through Gα 13', Blood, 138, pp. 579 - 579, http://dx.doi.org/10.1182/blood-2021-151753
    Journal articles | 2021
    Florido M; Chiu J; Hogg PJ, 2021, 'Influenza A Virus Hemagglutinin Is Produced in Different Disulfide-Bonded States', Antioxidants and Redox Signaling, 35, pp. 1081 - 1092, http://dx.doi.org/10.1089/ars.2021.0033
    Journal articles | 2021
    Pijning AE; Blyth MT; Coote ML; Passam F; Chiu J; Hogg PJ, 2021, 'An alternate covalent form of platelet αIIbβ3 integrin that resides in focal adhesions and has altered function', Blood, 138, pp. 1359 - 1372, http://dx.doi.org/10.1182/blood.2021012441
    Journal articles | 2021
    Xu X; Chiu J; Chen S; Fang C, 2021, 'Pathophysiological roles of cell surface and extracellular protein disulfide isomerase and their molecular mechanisms', British Journal of Pharmacology, 178, pp. 2911 - 2930, http://dx.doi.org/10.1111/bph.15493
    Journal articles | 2020
    Chen S; Xu X-L; Chiu J; Bowley S; Wu Y; Hogg PJ; Fang C, 2020, 'Extracellular Protein Disulfide Isomerase Cleaves Allosteric Disulfides in Histidine-Rich Glycoprotein to Regulate Thrombus Formation', Blood, 136, pp. 11 - 12, http://dx.doi.org/10.1182/blood-2020-137810
    Journal articles | 2020
    Lu ZF; Chiu J; Lee LR; Schindeler A; Jackson M; Ramaswamy Y; Dunstan CR; Hogg PJ; Zreiqat H, 2020, 'Reprogramming of human fibroblasts into osteoblasts by insulin-like growth factor-binding protein 7', Stem Cells Translational Medicine, 9, pp. 403 - 415, http://dx.doi.org/10.1002/sctm.19-0281
    Journal articles | 2019
    Chiu J; Hogg PJ, 2019, 'Allosteric disulfides: Sophisticated molecular structures enabling flexible protein regulation', Journal of Biological Chemistry, 294, pp. 2949 - 2960, http://dx.doi.org/10.1074/jbc.REV118.005604
    Journal articles | 2019
    Chiu J, 2019, 'Measurement of Redox States of the β3 Integrin Disulfide Bonds by Mass Spectrometry', Bio-protocol, 9, http://dx.doi.org/10.21769/BioProtoc.3156
    Journal articles | 2019
    Eriksson O; Chiu J; Hogg PJ; Atkinson JP; Liszewski MK; Flaumenhaft R; Furie B, 2019, 'Thiol isomerase ERp57 targets and modulates the lectin pathway of complement activation', Journal of Biological Chemistry, 294, pp. 4878 - 4888, http://dx.doi.org/10.1074/jbc.RA118.006792
    Journal articles | 2019
    Li J; Kim K; Jeong SY; Chiu J; Xiong B; Petukhov PA; Dai X; Li X; Andrews RK; Du X; Hogg PJ; Cho J, 2019, 'Platelet protein disulfide isomerase promotes glycoprotein ib&-mediated platelet-neutrophil interactions under thromboinflammatory conditions', Circulation, 139, pp. 1300 - 1319, http://dx.doi.org/10.1161/CIRCULATIONAHA.118.036323
    Journal articles | 2019
    Passam FJ; Chiu J, 2019, 'Allosteric disulphide bonds as reversible mechano-sensitive switches that control protein functions in the vasculature', Biophysical Reviews, 11, pp. 419 - 430, http://dx.doi.org/10.1007/s12551-019-00543-0
    Journal articles | 2019
    Su Z; Burchfield JG; Yang P; Humphrey SJ; Yang G; Francis D; Yasmin S; Shin SY; Norris DM; Kearney AL; Astore MA; Scavuzzo J; Fisher-Wellman KH; Wang QP; Parker BL; Neely GG; Vafaee F; Chiu J; Yeo R; Hogg PJ; Fazakerley DJ; Nguyen LK; Kuyucak S; James DE, 2019, 'Global redox proteome and phosphoproteome analysis reveals redox switch in Akt', Nature Communications, 10, http://dx.doi.org/10.1038/s41467-019-13114-4
    Journal articles | 2018
    Bekendam RH; Iyu D; Passam F; Stopa JD; De Ceunynck K; Muse O; Bendapudi PK; Garnier CL; Gopal S; Crescence L; Chiu J; Furie B; Panicot-Dubois L; Hogg PJ; Dubois C; Flaumenhaft R, 2018, 'Protein disulfide isomerase regulation by nitric oxide maintains vascular quiescence and controls thrombus formation', Journal of Thrombosis and Haemostasis, 16, pp. 2322 - 2335, http://dx.doi.org/10.1111/jth.14291
    Journal articles | 2018
    Passam F; Chiu J; Ju L; Pijning A; Jahan Z; Mor-Cohen R; Yeheskel A; Kolšek K; Thärichen L; Aponte-Santamaría C; Gräter F; Hogg PJ, 2018, 'Mechano-redox control of integrin de-adhesion', eLife, 7, http://dx.doi.org/10.7554/eLife.34843
    Journal articles | 2018
    Pijning AE; Chiu J; Yeo RX; Wong JWH; Hogg PJ, 2018, 'Identification of allosteric disulfides from labile bonds in X-ray structures', Royal Society Open Science, 5, http://dx.doi.org/10.1098/rsos.171058
    Journal articles | 2016
    Bekendam RH; Bendapudi PK; Lin L; Nag PP; Pu J; Kennedy DR; Feldenzer A; Chiu J; Cook KM; Furie B; Huang M; Hogg PJ; Flaumenhaft R, 2016, 'A substrate-driven allosteric switch that enhances PDI catalytic activity', Nature Communications, 7, http://dx.doi.org/10.1038/ncomms12579
    Journal articles | 2015
    Chiu J; Passam F; Butera D; Hogg PJ, 2015, 'Protein Disulfide Isomerase in Thrombosis', Seminars in Thrombosis and Hemostasis, 41, pp. 765 - 773, http://dx.doi.org/10.1055/s-0035-1564047
    Journal articles | 2014
    Butera D; Cook K; Chiu J; Wong JWH; Hogg PJ, 2014, 'Control of blood proteins by functional disulfide bonds', Blood, 123, pp. 2000 - 2007, http://dx.doi.org/10.1182/blood-2014-01-549816
    Journal articles | 2014
    Chiu J; Wong JWH; Gerometta M; Hogg PJ, 2014, 'Mechanism of dimerization of a recombinant mature vascular endothelial growth factor C', Biochemistry, 53, pp. 7 - 9, http://dx.doi.org/10.1021/bi401518b
    Journal articles | 2014
    Chiu J; Wong JWH; Hogg PJ, 2014, 'Redox regulation of Methionine aminopeptidase 2 activity', Journal of Biological Chemistry, 289, pp. 15035 - 15043, http://dx.doi.org/10.1074/jbc.M114.554253
    Journal articles | 2013
    Ng JY; Chiu J; Hogg PJ; Wong JWH, 2013, 'Tyrosine nitration moderates the peptidase activity of human methionyl aminopeptidase 2', Biochemical and Biophysical Research Communications, 440, pp. 37 - 42, http://dx.doi.org/10.1016/j.bbrc.2013.09.035
    Journal articles | 2012
    Chiu J; Dawes IW, 2012, 'Redox control of cell proliferation', Trends in Cell Biology, 22, pp. 592 - 601, http://dx.doi.org/10.1016/j.tcb.2012.08.002
    Journal articles | 2012
    Park D; Chiu J; Perrone G; Dilda P; Hogg PJ, 2012, 'The tumour metabolism inhibitors GSAO and PENAO react with cysteines 57 and 257 of mitochondrial adenine nucleotide translocase', Cancer Cell International, 12, pp. online open access, http://dx.doi.org/10.1186/1475-2867-12-11
    Journal articles | 2011
    Chiu J; Tactacan CM; Tan S; Lin RCY; Wouters MA; Dawes IW, 2011, 'Cell cycle sensing of oxidative stress in Saccharomyces cerevisiae by oxidation of a specific cysteine residue in the transcription factor Swi6p', The Journal of Biological Chemistry, 286, pp. 5204 - 5214, http://dx.doi.org/10.1074/jbc.M110.172973
    Journal articles | 2009
    Tsoi BM; Beckhouse AG; Gelling CL; Raftery MJ; Chiu J; Tsoi AM; Lauterbach L; Rogers PL; Higgins VJ; Dawes IW, 2009, 'Essential role of one-carbon metabolism and Gcn4p and Bas1p transcriptional regulators during adaptation to anaerobic growth of Saccharomyces cerevisiae', The Journal of Biological Chemistry, 284, pp. 11205 - 11215
    Journal articles | 2008
    Chiu II; Tillett D; Dawes IW; March PE, 2008, 'Site-directed, Ligase-Independent Mutagenesis (SLIM) for highly efficient mutagenesis of plasmids greater than 8kb', Journal of Microbiological Methods, 73, pp. 195 - 198, http://dx.doi.org/10.1016/j.mimet.2008.02.013
    Journal articles | 2008
    Fong C; Temple MD; Alic N; Chiu II; Durchdewald M; Thorpe GW; Higgins VJ; Dawes IW, 2008, 'Oxidant-induced cell-cycle delay in Saccharomyces cerevisiae: the involvement of the SWI6 transcription factor', FEMS Yeast Research, 8, pp. 386 - 399, http://dx.doi.org/10.1111/j.1567-1364.2007.00349.x
    Journal articles | 2006
    Chiu J; Tillett D; March PE, 2006, 'Coexpression of the subunits of T7 DNA polymerase from an artificial operon allows one-step purification of active gp5/Trx complex', Protein Expression and Purification, 47, pp. 264 - 272
    Journal articles | 2006
    Chiu J; Tillett D; March PE, 2006, 'Mutation Of PHE102 to Ser in the carboxyl terminal helix of Escherichia coli thioredoxin affects the stability and processivity of T7 DNA polymerase', Proteins - Structure Function and Bioinformatics, 64, pp. 477 - 485
    Journal articles | 2004
    Chiu J; March PE; Lee R; Tillett D, 2004, 'Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h', Nucleic Acids Research, 32, pp. e174 - e174, http://www.sciencedirect.com/science/article/B6WVB-4F08SS4-2HR/2/b98d5e9c1bf5baf1397486a6cec49834
  • Preprints | 2022
    Dupuy A; Santamaría CA; Yeheskel A; Gräter F; Hogg PJ; Passam FH; Chiu J, 2022, Mechano-redox control of Mac-1 de-adhesion from ICAM-1 by protein disulfide isomerase promotes directional movement of neutrophils under flow, http://dx.doi.org/10.1101/2022.03.29.486223
    Preprints | 2022
    Kutzki F; Butera D; Lay AJ; Maag D; Chiu J; Woon H-G; Kubař T; Elstner M; Aponte-Santamaría C; Hogg PJ; Gräter F, 2022, Dynamic Disulfide Bond Topologies in von-Willebrand-Factor’s C4-Domain Undermine Platelet Binding, http://dx.doi.org/10.1101/2022.08.20.504523
    Conference Abstracts | 2018
    Maiocchi S; Al-Daher S; Mcfadyen J; Ju A; Alwis I; Chiu J; Passam F; Hogg P; Yuan Y; Jackson S, 2018, 'Investigation of redox modifications of platelet integrin GPIIb-IIIa in diabetes', in FREE RADICAL BIOLOGY AND MEDICINE, ELSEVIER SCIENCE INC, IL, Chicago, Vol. 128, pp. S55 - S55, presented at 25th Annual Meeting of the Society-for-Redox-Biology-and-Medicine (SfRBM), IL, Chicago, 14 November 2018 - 17 November 2018, http://dx.doi.org/10.1016/j.freeradbiomed.2018.10.102
    Conference Abstracts | 2015
    Kim K; Li J; Andrews RK; Chiu J; Hogg P; Cho J, 2015, 'Platelet Surface PDI Controls the Ligand-Binding Function of Glycoprotein Ibalpha and Platelet-Neutrophil Interactions Under Thromboinflammatory Conditions', in BLOOD, AMER SOC HEMATOLOGY, FL, Orlando, Vol. 126, presented at 57th Annual Meeting of the American-Society-of-Hematology, FL, Orlando, 05 December 2015 - 08 December 2015, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000368019000299&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2008
    Fan SW; George R; Haworth N; Chiu J; Dawes IW; Wouters MA, 2008, 'Prediction of Redox-regulated Cys in Yeast', in ICCB 2008: PROCEEDINGS OF THE 9TH INTERNATIONAL CONGRESS ON CELL BIOLOGY, Medimond S R L, 40128 Bologna

2022 NSW Cardiovascular Research Network – Professional Development Award

2021-2023 Co-investigator of the NSW Cardiovascular Capacity EMCR Grant

2019 Charles Perkin Centre EMCR Seed Funding

2018 Charles Perkin Centre EMCR Seed Funding

2018 Sydney Medical School Professor Tony Basten Fellowship

2017-2019 Sydney Medical School Foundation Helen and Robert Ellis Fellowship

 

2023 NSW Cardiovascular Research Network Rising Star seminar series presenter

2022 15th World Congress on Inflammation (Rome) Oral Communication Award

2021 APSTH and JSTH Joint Symposium Outstanding Young Scientist Award

My Research Supervision

PhD students

2019-2023 Alexander Dupuy (USYD)

2018-2022 Aster Pijning (USYD)

2015-2018 Reichelle Yeo (UNSW/USYD)

Honours students

2021 Dasol Yoo (USYD)

2020 Kenny Lu (USYD)