Ms Leeba Chacko
Dr. Leeba Ann Chacko is a Research Associate at the Katharina Gaus Light Microscopy Facility at UNSW, based within the Mark Wainwright Analytical Centre, specialising in advanced fluorescence imaging and quantitative image analysis. Her work spans confocal, spinning disk, FLIM, multiphoton, and STED microscopy, with a particular focus on spectral imaging and unmixing using Leica SpectraPlex, alongside end to end instrument support and researcher training.
She completed her PhD in Anatomy at UNSW in 2025, where she investigated how mitochondrial function regulates cellular growth kinetics using live cell imaging in microfluidic devices, combining MATLAB based quantitative analysis with Python workflows and deep learning segmentation using Cellpose. Across earlier roles at the Indian Institute of Science, the National University of Singapore, and Memorial Sloan Kettering Cancer Center, she developed expertise in live cell microscopy and quantitative analysis applied to cytoskeletal regulation and mitochondrial dynamics.
At KGLMF, Dr. Chacko supports diverse biomedical imaging projects by developing robust acquisition and analysis pipelines, benchmarking advanced methodologies, and translating quantitative microscopy approaches into practical workflows for researchers at UNSW and collaborating institutions.
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SBMS Paper of the month Award November 2025
Publications
Journal articles | 2025
2025, 'Mitochondrial function regulates cell growth kinetics to maintain mitochondrial homeostasis', Current Biology, 35(21), pp. 5278 to 5288.e4, http://dx.doi.org/10.1016/j.cub.2025.09.046
Journal articles | 2023
2023, 'Microtubule mitochondrial attachment facilitates cell division symmetry and mitochondrial partitioning in fission yeast', Journal of Cell Science, 136(1), pp. jcs260705, http://dx.doi.org/10.1242/jcs.260705
Journal articles | 2021
2021, 'Mitochondrial dynamics, positioning and function mediated by cytoskeletal interactions', Cellular and Molecular Life Sciences, 78(8), pp. 3969 to 3986, http://dx.doi.org/10.1007/s00018-021-03762-5
Journal articles | 2019
2019, 'Quantification of mitochondrial dynamics in fission yeast', Bio protocol, 9(23), pp. e3450, http://dx.doi.org/10.21769/BioProtoc.3450
2019, 'Cortical tethering of mitochondria by the anchor protein Mcp5 enables uniparental inheritance', Journal of Cell Biology, 218(11), pp. 3560 to 3571, http://dx.doi.org/10.1083/jcb.201901108
2019, 'Association of mitochondria with microtubules inhibits mitochondrial fission by precluding assembly of the fission protein Dnm1', Journal of Biological Chemistry, 294(10), pp. 3385 to 3396, http://dx.doi.org/10.1074/jbc.RA118.006799
Journal articles | 2017
2017, 'Role of Microtubules in Osteogenic Differentiation of Mesenchymal Stem Cells on 3D Nanofibrous Scaffolds', ACS Biomaterials Science and Engineering, 3(4), pp. 551 to 559, http://dx.doi.org/10.1021/acsbiomaterials.6b00725