Conjoint Senior Lecturer

Ms Lei Zhang

Medicine & Health
School of Clinical Medicine
  • Journal articles | 2023
    2023, 'Monitoring AKT activity and targeting in live tissue and disease contexts using a real-time Akt-FRET biosensor mouse.', Sci Adv, 9, pp. eadf9063, http://dx.doi.org/10.1126/sciadv.adf9063
    Journal articles | 2022
    2022, 'Central NPFF signalling is critical in the regulation of glucose homeostasis', Molecular Metabolism, 62, pp. 101525, http://dx.doi.org/10.1016/j.molmet.2022.101525
    Journal articles | 2022
    2022, 'Interfacially Super-Assembled Tyramine-Modified Mesoporous Silica-Alumina Oxide Heterochannels for Label-Free Tyrosinase Detection', Analytical Chemistry, 94, pp. 2589 - 2596, http://dx.doi.org/10.1021/acs.analchem.1c04825
    Journal articles | 2022
    2022, 'NPFF signalling is critical for thermosensory and dietary regulation of thermogenesis', Neuropeptides, 96, http://dx.doi.org/10.1016/j.npep.2022.102292
    Journal articles | 2022
    2022, 'NPY derived from AGRP neurons controls feeding via Y1 and energy expenditure and food foraging behaviour via Y2 signalling', Molecular Metabolism, 59, pp. 101455, http://dx.doi.org/10.1016/j.molmet.2022.101455
    Journal articles | 2022
    2022, 'The Effect of Dietary Fat and Sucrose on Cognitive Functioning in Mice Lacking Insulin Signaling in Neuropeptide Y Neurons', Frontiers in Physiology, 13, pp. 841935, http://dx.doi.org/10.3389/fphys.2022.841935
    Journal articles | 2022
    2022, 'The effect of insulin receptor deletion in neuropeptide Y neurons on hippocampal dependent cognitive function in aging mice', Journal of Integrative Neuroscience, 21, pp. 6, http://dx.doi.org/10.31083/j.jin2101006
    Journal articles | 2021
    2021, 'Dynamic regional alterations in mouse brain neuronal activity following short-term changes in energy balance', Obesity, 29, pp. 1650 - 1663, http://dx.doi.org/10.1002/oby.23253
    Journal articles | 2021
    2021, 'Lack of neuropeptide FF signalling in mice leads to reduced repetitive behavior, altered drinking behavior, and fuel type selection', FASEB Journal, 35, pp. e21980, http://dx.doi.org/10.1096/fj.202100703R
    Journal articles | 2021
    2021, 'Lack of peptide YY signaling in mice disturbs gut microbiome composition in response to high-fat diet', FASEB Journal, 35, pp. e21435, http://dx.doi.org/10.1096/fj.202002215R
    Journal articles | 2021
    2021, 'Method for voluntary oral administration of drugs in mice', STAR Protocols, 2, pp. 100330, http://dx.doi.org/10.1016/j.xpro.2021.100330
    Journal articles | 2021
    2021, 'Ninjin'yoeito modulates feeding and activity under negative energy balance conditions via the NPY system', Neuropeptides, 87, http://dx.doi.org/10.1016/j.npep.2021.102149
    Journal articles | 2021
    2021, 'Ninjin'yoeito, a herbal medicine, enhances glucose tolerance in mice', Neuropeptides, 88, http://dx.doi.org/10.1016/j.npep.2021.102150
    Journal articles | 2021
    2021, 'Peripheral-specific Y1 receptor antagonism increases thermogenesis and protects against diet-induced obesity', Nature Communications, 12, pp. 2622, http://dx.doi.org/10.1038/s41467-021-22925-3
    Journal articles | 2021
    2021, 'The distribution of Neuropeptide FF and Neuropeptide VF in central and peripheral tissues and their role in energy homeostasis control', Neuropeptides, 90, http://dx.doi.org/10.1016/j.npep.2021.102198
    Journal articles | 2020
    2020, 'A Vagal-NTS Neural Pathway that Stimulates Feeding', Current Biology, 30, pp. 3986 - 3998.e5, http://dx.doi.org/10.1016/j.cub.2020.07.084
    Journal articles | 2020
    2020, 'Leptin signalling on arcuate NPY neurones controls adiposity independent of energy balance or diet composition', Journal of Neuroendocrinology, 32, http://dx.doi.org/10.1111/jne.12898
    Journal articles | 2020
    2020, 'Neuropeptide Y Regulation of Energy Partitioning and Bone Mass During Cold Exposure', Calcified Tissue International, 107, pp. 510 - 523, http://dx.doi.org/10.1007/s00223-020-00745-9
    Journal articles | 2019
    2019, 'Amygdala NPY Circuits Promote the Development of Accelerated Obesity under Chronic Stress Conditions', Cell Metabolism, 30, pp. 111 - 128.e6, http://dx.doi.org/10.1016/j.cmet.2019.04.001
    Journal articles | 2019
    2019, 'Chronic stress and energy homoeostasis', Aging, 11, pp. 9963 - 9964, http://dx.doi.org/10.18632/aging.102500
    Journal articles | 2019
    2019, 'Coordinated activation of central amygdala–arcuate nucleus NPY circuits are required for the development of stress-induced obesity', Obesity Research & Clinical Practice, 13, pp. 284 - 284, http://dx.doi.org/10.1016/j.orcp.2018.11.141
    Journal articles | 2019
    2019, 'Distinct populations of Arc NPY neurons control specific aspects of energy homeostasis', Obesity Research & Clinical Practice, 13, pp. 277 - 277, http://dx.doi.org/10.1016/j.orcp.2018.11.123
    Journal articles | 2019
    2019, 'Osteoglycin, a novel coordinator of bone and glucose homeostasis', Obesity Research & Clinical Practice, 13, pp. 256 - 256, http://dx.doi.org/10.1016/j.orcp.2018.11.063
    Journal articles | 2019
    2019, 'Regulation of feeding-related behaviors by arcuate neuropeptide Y neurons', Endocrinology, 160, pp. 1411 - 1420, http://dx.doi.org/10.1210/en.2019-00056
    Journal articles | 2018
    Farzi A; Lau J; Ip CK; Qi Y; Shi YC; Zhang L; Tasan R; Sperk G; Herzog H, 2018, 'Arcuate nucleus and lateral hypothalamic cart neurons in the mouse brain exert opposing effects on energy expenditure', eLife, 7, http://dx.doi.org/10.7554/eLife.36494
    Journal articles | 2018
    Nguyen AD; Lee NJ; Wee NK Y; Zhang L; Enriquez RF; Khor EC; Nie T; Wu D; Sainsbury A; Baldock PA; Herzog H, 2018, 'Uncoupling protein-1 is protective of bone mass under mild cold stress conditions', Bone, 106, pp. 167 - 178, http://dx.doi.org/10.1016/j.bone.2015.05.037
    Journal articles | 2018
    2018, 'Diet-induced adaptive thermogenesis requires neuropeptide FF receptor-2 signalling', Nature Communications, 9, http://dx.doi.org/10.1038/s41467-018-06462-0
    Journal articles | 2018
    2018, 'Osteoglycin, an Osteoblast-derived Regulator of Bone Mass and Glucose Homeostasis', Molecular Metabolism, 13, pp. 30 - 44, http://dx.doi.org/10.1016/j.molmet.2018.05.004
    Journal articles | 2017
    Loh K; Zhang L; Brandon A; Wang Q; Begg D; Qi Y; Fu M; Kulkarni R; Teo J; Baldock P; Bruning JC; Cooney G; Neely GG; Herzog H, 2017, 'Corrigendum to “Insulin controls food intake and energy balance via NPY neurons” [Mol Metabol 6 (2017) 574–584], (S2212877817301552), (10.1016/j.molmet.2017.03.013))', Molecular Metabolism, 6, pp. 1562, http://dx.doi.org/10.1016/j.molmet.2017.06.017
    Journal articles | 2017
    Nobis M; Herrmann D; Warren SC; Kadir S; Leung W; Killen M; Magenau A; Stevenson D; Lucas MC; Reischmann N; Vennin C; Conway JR W; Boulghourjian A; Zaratzian A; Law AM; Gallego-Ortega D; Ormandy CJ; Walters SN; Grey ST; Bailey J; Chtanova T; Quinn JM W; Baldock PA; Croucher PI; Schwarz JP; Mrowinska A; Zhang L; Herzog H; Masedunskas A; Hardeman EC; Gunning PW; del Monte-Nieto G; Harvey RP; Samuel MS; Pajic M; McGhee EJ; Johnsson AK E; Sansom OJ; Welch HC E; Morton JP; Strathdee D; Anderson KI; Timpson P, 2017, 'A RhoA-FRET Biosensor Mouse for Intravital Imaging in Normal Tissue Homeostasis and Disease Contexts', Cell Reports, 21, pp. 274 - 288, http://dx.doi.org/10.1016/j.celrep.2017.09.022
    Journal articles | 2017
    Qi Y; Purtell L; Fu M; Sengmany K; Loh K; Zhang L; Zolotukhin S; Sainsbury A; Campbell L; Herzog H, 2017, 'Ambient temperature modulates the effects of the Prader-Willi syndrome candidate gene Snord116 on energy homeostasis', Neuropeptides, 61, pp. 87 - 93, http://dx.doi.org/10.1016/j.npep.2016.10.006
    Journal articles | 2017
    Qi Y; Purtell L; Fu M; Zhang L; Zolotukhin S; Campbell L; Herzog H, 2017, 'Hypothalamus Specific Re-Introduction of SNORD116 into Otherwise Snord116 Deficient Mice Increased Energy Expenditure', Journal of Neuroendocrinology, 29, http://dx.doi.org/10.1111/jne.12457
    Journal articles | 2017
    2017, 'Circulating neuregulin 4 concentrations in patients with newly diagnosed type 2 diabetes: a cross-sectional study', Endocrine, 57, pp. 535 - 538, http://dx.doi.org/10.1007/s12020-017-1324-3
    Journal articles | 2017
    2017, 'Insulin controls food intake and energy balance via NPY neurons', Molecular Metabolism, 6, pp. 574 - 584, http://dx.doi.org/10.1016/j.molmet.2017.03.013
    Journal articles | 2016
    Khor EC; Yulyaningsih E; Driessler F; Kovaĉić N; Wee NK Y; Kulkarni RN; Lee NJ; Enriquez RF; Xu J; Zhang L; Herzog H; Baldock PA, 2016, 'The y6 receptor suppresses bone resorption and stimulates bone formation in mice via a suprachiasmatic nucleus relay', Bone, 84, pp. 139 - 147, http://dx.doi.org/10.1016/j.bone.2015.12.011
    Journal articles | 2016
    Lengkeek NA; Roberts MP; Zhang L; Lee IC J; Fookes CJ R; Dikic B; Herzog H; Katsifis A; Greguric I, 2016, 'Synthesis and Binding Affinity of Fluorine Containing NG-acyl and -sulfonyl BIBP3226 Derivatives: Ligands for the NPY Y1 Receptor', Australian Journal of Chemistry, 69, pp. 746 - 752, http://dx.doi.org/10.1071/CH15569
    Journal articles | 2016
    Liu M; Richardson RR; Mountford SJ; Zhang L; Tempone MH; Herzog H; Holliday ND; Thompson PE, 2016, 'Identification of a Cyanine-Dye Labeled Peptidic Ligand for Y1R and Y4R, Based upon the Neuropeptide y C-Terminal Analogue, BVD-15', Bioconjugate Chemistry, 27, pp. 2166 - 2175, http://dx.doi.org/10.1021/acs.bioconjchem.6b00376
    Journal articles | 2016
    Qi Y; Purtell L; Fu M; Lee NJ; Aepler J; Zhang L; Loh K; Enriquez RF; Baldock PA; Zolotukhin S; Campbell LV; Herzog H, 2016, 'Snord116 is critical in the regulation of food intake and body weight', Scientific Reports, 6, http://dx.doi.org/10.1038/srep18614
    Journal articles | 2016
    Seimon RV; Shi YC; Slack K; Lee K; Fernando HA; Nguyen AD; Zhang L; Lin S; Enriquez RF; Lau J; Herzog H; Sainsbury A, 2016, 'Intermittent Moderate Energy Restriction Improves Weight Loss Efficiency in Diet-Induced Obese Mice', PLoS ONE, 11, http://dx.doi.org/10.1371/journal.pone.0145157
    Journal articles | 2016
    Wang QP; Lin YQ; Zhang L; Wilson YA; Oyston LJ; Cotterell J; Qi Y; Khuong TM; Bakhshi N; Planchenault Y; Browman DT; Lau MT; Cole TA; Wong AC N; Simpson SJ; Cole AR; Penninger JM; Herzog H; Neely GG, 2016, 'Sucralose Promotes Food Intake through NPY and a Neuronal Fasting Response', Cell Metabolism, 24, pp. 75 - 90, http://dx.doi.org/10.1016/j.cmet.2016.06.010
    Journal articles | 2016
    2016, 'Developing superior peptide conjugates for studying Y receptor biology', Neuropeptides, 55, pp. 10 - 10, http://dx.doi.org/10.1016/j.npep.2015.11.024
    Journal articles | 2016
    2016, 'Hypothalamic neuropeptide FF receptor-2 signaling is critical for the regulation of energy homeostasis', Neuropeptides, 55, pp. 13 - 13, http://dx.doi.org/10.1016/j.npep.2015.11.034
    Journal articles | 2016
    2016, 'Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue', Cell Reports, 14, pp. 152 - 167, http://dx.doi.org/10.1016/j.celrep.2015.12.020
    Journal articles | 2016
    2016, 'The y6 receptor suppresses bone resorption and stimulates bone formation in mice', Neuropeptides, 55, pp. 24 - 24, http://dx.doi.org/10.1016/j.npep.2015.11.068
    Journal articles | 2015
    Bradley EA; Zhang L; Genders AJ; Richards SM; Rattigan S; Keske MA, 2015, 'Enhancement of insulin-mediated rat muscle glucose uptake and microvascular perfusion by 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside', Cardiovascular Diabetology, 14, http://dx.doi.org/10.1186/s12933-015-0251-y
    Journal articles | 2015
    Tactacan CM; Phua YW; Liu L; Zhang L; Humphrey ES; Cowley M; Pinese M; Biankin AV; Daly RJ, 2015, 'The pseudokinase SgK223 promotes invasion of pancreatic ductal epithelial cells through JAK1/Stat3 signaling', Molecular Cancer, 14, http://dx.doi.org/10.1186/s12943-015-0412-3
    Journal articles | 2015
    Wu J; Xiao J; Li T; Li X; Sun H; Chow EP; Lu Y; Tian T; Li X; Wang Q; Zhuang X; Zhang L, 2015, 'A cross-sectional survey on the health status and the health-related quality of life of the elderly after flood disaster in Bazhong city, Sichuan, China', BMC public health, 15, pp. 163, http://dx.doi.org/10.1186/s12889-015-1402-5
    Journal articles | 2015
    Zhang L; Phanuphak N; Henderson K; Nonenoy S; Srikaew S; Shattock AJ; Kerr CC; Omune B; van Griensven F; Osornprasop S; Oelrichs R; Ananworanich J; Wilson DP, 2015, 'Scaling up of HIV treatment for men who have sex with men in Bangkok: a modelling and costing study', The Lancet HIV, 2, pp. e200 - e207, http://dx.doi.org/10.1016/S2352-3018(15)00020-X
    Journal articles | 2014
    Baldock PA; Lin S; Zhang L; Karl T; Shi Y; Driessler F; Zengin A; Hörmer B; Lee NJ; Wong IP L; Lin EJ D; Enriquez RF; Stehrer B; During MJ; Yulyaningsih E; Zolotukhin S; Ruohonen ST; Savontaus E; Sainsbury A; Herzog H, 2014, 'Neuropeptide Y attenuates stress-induced bone loss through suppression of noradrenaline circuits', Journal of Bone and Mineral Research, 29, pp. 2238 - 2249, http://dx.doi.org/10.1002/jbmr.2205
    Journal articles | 2014
    Mountford SJ; Liu M; Zhang L; Groenen M; Herzog H; Holliday ND; Thompson PE, 2014, 'Synthetic routes to the Neuropeptide y Y1 receptor antagonist 1229U91 and related analogues for SAR studies and cell-based imaging', Organic and Biomolecular Chemistry, 12, pp. 3271 - 3281, http://dx.doi.org/10.1039/c4ob00176a
    Journal articles | 2014
    Yulyaningsih E; Loh K; Lin S; Lau J; Zhang L; Shi Y; Berning BA; Enriquez R; Driessler F; Macia L; Khor EC; Qi Y; Baldock P; Sainsbury A; Herzog H, 2014, 'Pancreatic polypeptide controls energy homeostasis via Npy6r signaling in the suprachiasmatic nucleus in mice', Cell Metabolism, 19, pp. 58 - 72, http://dx.doi.org/10.1016/j.cmet.2013.11.019
    Journal articles | 2014
    2014, 'Double deletion of orexigenic neuropeptide Y and dynorphin results in paradoxical obesity in mice', Neuropeptides, 48, pp. 143 - 151, http://dx.doi.org/10.1016/j.npep.2014.03.001
    Journal articles | 2014
    2014, 'Stress- and diet-induced fat gain is controlled by NPY in catecholaminergic neurons', Molecular Metabolism, 3, pp. 581 - 591, http://dx.doi.org/10.1016/j.molmet.2014.05.001
    Journal articles | 2013
    Liu M; Mountford SJ; Zhang L; Lee IC; Herzog H; Thompson PE, 2013, 'Synthesis of BVD15 peptide analogues as models for radioligands in tumour imaging', International Journal of Peptide Research and Therapeutics, 19, pp. 33 - 41, http://dx.doi.org/10.1007/s10989-012-9330-z
    Journal articles | 2013
    Shi YC; Lau J; Lin Z; Zhang H; Zhai L; Sperk G; Heilbronn R; Mietzsch M; Weger S; Huang XF; Enriquez RF; Baldock PA; Zhang L; Sainsbury A; Herzog H; Lin S, 2013, 'Arcuate NPY controls sympathetic output and BAT function via a relay of tyrosine hydroxylase neurons in the PVN', Cell Metabolism, 17, pp. 236 - 248, http://dx.doi.org/10.1016/j.cmet.2013.01.006
    Journal articles | 2013
    2013, 'Neuropeptide FF receptor-2 signalling is critical for diet-induced thermogenesis regulation', Obesity Research & Clinical Practice, 7, pp. e28 - e29, http://dx.doi.org/10.1016/j.orcp.2013.12.556
    Journal articles | 2013
    2013, 'Neuropeptide Y mediates the short-term hypometabolic effect of estrogen deficiency in mice', International Journal of Obesity, 37, pp. 390 - 398, http://dx.doi.org/10.1038/ijo.2012.71
    Journal articles | 2012
    2012, 'Critical role of Neuropeptide FF receptor 2 in the regulation of energy balance and glucose homeostasis revealed in mice', Obesity Research & Clinical Practice, 6, pp. 17 - 17, http://dx.doi.org/10.1016/j.orcp.2012.08.035
    Journal articles | 2012
    2012, 'NPY modulates PYY function in the regulation of energy balance and glucose homeostasis', Diabetes Obesity and Metabolism, 14, pp. 727 - 736, http://dx.doi.org/10.1111/j.1463-1326.2012.01592.x
    Journal articles | 2012
    2012, 'Neuropeptide y1 receptor in immune cells regulates inflammation and insulin resistance associated with diet-induced obesity', Diabetes, 61, pp. 3228 - 3238, http://dx.doi.org/10.2337/db12-0156
    Journal articles | 2012
    2012, 'Role of the hypothalamus in the neuroendocrine regulation of body weight and composition during energy deficit', Obesity Reviews, 13, pp. 234 - 257, http://dx.doi.org/10.1111/j.1467-789X.2011.00948.x
    Journal articles | 2012
    2012, 'The role of NPY and Ghrelin in anorexia nervosa', Current Pharmaceutical Design, 18, pp. 4766 - 4778, http://dx.doi.org/10.2174/138161212803216988
    Journal articles | 2011
    2011, 'Central neuropeptide Y infusion and melanocortin 4 receptor antagonism inhibit thyrotropic function by divergent pathways', Neuropeptides, 45, pp. 407 - 415, http://dx.doi.org/10.1016/j.npep.2011.07.009
    Journal articles | 2011
    2011, 'NPY receptors as potential targets for anti-obesity drug development', British Journal of Pharmacology, 163, pp. 1170 - 1202, http://dx.doi.org/10.1111/j.1476-5381.2011.01363.x
    Journal articles | 2011
    2011, 'The neuropeptide y system: Pathophysiological and therapeutic implications in obesity and cancer', Pharmacology and Therapeutics, 131, pp. 91 - 113, http://dx.doi.org/10.1016/j.pharmthera.2011.03.011
    Journal articles | 2010
    Mountford J; Zhang L; Herzog H; Thompson P, 2010, 'Synthesis of Neuropeptide Y analogues as PET imaging agents', JOURNAL OF PEPTIDE SCIENCE, 16, pp. 89 - 89, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000281610800213&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Journal articles | 2010
    2010, 'Additive actions of the cannabinoid and neuropeptide Y systems on adiposity and lipid oxidation', Diabetes Obesity and Metabolism, 12, pp. 591 - 603, http://dx.doi.org/10.1111/j.1463-1326.2009.01193.x
    Journal articles | 2010
    2010, 'Peptide YY is critical for acylethanolamine receptor Gpr119-induced activation of gastrointestinal mucosal responses', Cell Metabolism, 11, pp. 532 - 542, http://dx.doi.org/10.1016/j.cmet.2010.04.014
    Journal articles | 2010
    2010, 'Role of the arcuate nucleus of the hypothalamus in regulation of body weight during energy deficit', Molecular and Cellular Endocrinology, 316, pp. 109 - 119, http://dx.doi.org/10.1016/j.mce.2009.09.025
    Journal articles | 2009
    Yuen DY C; Dwyer RM; Matthews VB; Zhang L; Drew BG; Neill B; Kingwell BA; Clark MG; Rattigan S; Febbraio MA, 2009, 'Interleukin-6 attenuates insulin-mediated increases in endothelial cell signaling but augments skeletal muscle insulin action via differential effects on tumor necrosis factor-α expression', Diabetes, 58, pp. 1086 - 1095, http://dx.doi.org/10.2337/db08-0775
    Journal articles | 2007
    Lin EJ D; Zhang L; Sainsbury A; Herzog H, 2007, 'Y2, Y4 receptors and obesity', Expert Review of Endocrinology and Metabolism, 2, pp. 163 - 173, http://dx.doi.org/10.1586/17446651.2.2.163
    Journal articles | 2005
    2005, 'Acute glucosamine-induced insulin resistance in muscle in vivo is associated with impaired capillary recruitment', Diabetologia, 48, pp. 2131 - 2139, http://dx.doi.org/10.1007/s00125-005-1887-z
    Journal articles | 2005
    2005, 'Microvascular flow routes in muscle controlled by vasoconstrictors', Microvascular Research, 70, pp. 7 - 16, http://dx.doi.org/10.1016/j.mvr.2005.06.001
    Journal articles | 2004
    2004, 'Insulin Sensitivity of Muscle Capillary Recruitment In Vivo', Diabetes, 53, pp. 447 - 453, http://dx.doi.org/10.2337/diabetes.53.2.447
    Journal articles | 2003
    2003, 'TNF-α acutely inhibits vascular effects of physiological but not high insulin or contraction', American Journal of Physiology - Endocrinology and Metabolism, 285, http://dx.doi.org/10.1152/ajpendo.00119.2003
  • Conference Papers | 2009
    Mitchell N; Zhang L; Lin S; Sainsbury A; Herzog H, 2009, 'DELETION OF Y1 AND Y5 RECEPTORS INDUCE A HYPOPHAGIC PHENOTYPE', in NEUROPEPTIDES, CHURCHILL LIVINGSTONE, AUSTRIA, Salzburg, pp. 410 - 410, presented at Joint Meeting of the European-Neuropeptides-Club/Summer Neuropeptides Conference, AUSTRIA, Salzburg, 20 July 2009 - 23 July 2009, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000271890700010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1