GE & BioRad mini & large format SDS-PAGE systems
Description
Proteomic work can involve the use of one-dimensional (SDS-PAGE) or two-dimensional (2-D PAGE or 2-DE) polyacrylamide gel electrophoresis.
SDS-PAGE can also be used independently. Proteins and peptides are separated within the gel according to the % acrylamide and crosslinker, given as %T and %C.
Peptides can also be separated using a compatible buffer system. A typical buffer system is Tris/glycine/SDS, however other combinations may be more suitable to your sample. Native and denaturing gels can be prepared. Choice will depend on sample requirements.
Specifications
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- High mass protein separations (homogeneous): 7%T, 2.7%C
- Los mass protein separations (homogeneous): up to 15%T, 2.7%C
- Gradient gels: 4-20%T
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- First dimension electrophoresis with a pH gradient and high urea
- Second dimension separation in SDS-PAGE
Applications
- Protein separations
- Post-translational modification studies
- Sample preparation prior to mass spectrometry
Instrument location
Bioanalytical Mass Spectrometry Facility
Instrument location:
Room 401, Level 4 North West
Wallace Wurth Building (C27)
UNSW Sydney, NSW 2033
Phone: 02 9385 1717
Email: bmsf@unsw.edu.au
Dr Valerie Wasinger
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Phone
02 9385 1678 -
Email
v.wasinger@unsw.edu.au
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