GE IPGphor/Multiphor Isoelectric Focusing Systems (C27)
IEF separates molecules and other biomolecules based on their isoelectric point (pI)
Description
Isoelectric focusing (IEF) is an analytical technique used to separate proteins and other biomolecules based on their isoelectric point (pI).
This system enables analytical or preparative separations of proteins and peptides by IEF, native or SDS-PAGE, and 2-D PAGE.
Specifications
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- IEF gels (various lengths possible: 7, 13 or 18 cm)
- pH gradients:
- Broad range pH 3-10
- Mid-range pH 4-7, 6-11
- Narrow range (0.01 pH unit separating power)
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- First dimension of 2-D electrophoresis
- Second dimension using SDS-PAGE
Applications
- Fractionation of complex biological samples in pathway and systems analysis
- Quantitation of protein effector molecules
- Elucidation of protein modifications
- Sample clean-up for mass spectrometry analysis
Instrument location
Bioanalytical Mass Spectrometry Facility
Room 402, Level 4 North West
Wallace Wurth Building (C27)
UNSW Sydney, NSW 2033
Phone: 02 9385 1717
Email: bmsf@unsw.edu.au
Dr Valerie Wasinger
Senior Research Scientist
-
Phone
02 9385 1678 -
Email
v.wasinger@unsw.edu.au
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