GE Healthcare iTC200 Isothermal Calorimeter (E26)
Description
The Isothermal Calorimeter (iTC) investigates the production or absorption of heat from molecular interactions, reaction kinetics, and thermodynamics between molecules or a chemical reaction.
The iTC is the only equipment that can directly measure the energetics of biochemical processes involving interaction between two molecules such as a receptor and a ligand. A receptor can be any biopolymer such as a protein/enzyme, nucleic acid, carbohydrate, lipid etc, whereas a ligand can be either a large or small molecule (such as a metal, drug, inhibitor, activator, toxin, substrate, protein/peptide, sugar etc).
In addition to determining the affinity and stoichiometry of molecular binding, iTC can precisely measure the free energy, enthalpy, entropy and heat-capacity changes associated with binding and reaction.
Specifications
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- 0.2 mL of sample
- 0.04 mL of titrant
- A heat signal of 0.2 µcal/sec is detectable
- Analysis time < 1 hour
- Can be upgraded to provide high sample throughput for up to 50 samples/day
- Can process 384 samples unattended
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- UNSW and affiliated organisations:
- $300 do it yourself (DIY)
- $600 fee for service (FFS)
- Training rate & staff time $60/hour, per project
- External academics:
- $600 (DIY)
- $1200 (FFS)
- Training & staff time $180/hour, per project.
- UNSW and affiliated organisations:
Applications
- Binding studies between a biopolymer and a ligand
- Compound screening in drug discovery
Instrument location
Bioanalytical Mass Spectrometry Facility
Room 2002, Level 2 South
Biosciences Precinct (E26)
UNSW Sydney, NSW 2033
Phone: 02 9385 1717
Email: bmsf@unsw.edu.au
Dr Anne Poljak
-
Phone
02 9385 1994 -
Email
a.poljak@unsw.edu.au
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