Thermo QExactive HF
Description
The QE HF is a hybrid instrument that incorporates both quadrupole and Orbitrap mass analysers. This system can be set up in nano- and high-flow rates for proteomics, metabolomics and lipidomics analyses.
Both 1D and 2D nano liquid chromatography (LC) separations can be interfaced to this instrument. This platform allows the benefits of Orbitrap mass analysis to be exploited in highly sensitive experiments, as well as the rapid mass selection capabilities of quadrupoles.
Detailed insights into analyte molecule structural characteristics can be obtained via tandem mass spectrometry (MS/MS) experiments.
Quantitative analyses are possible via, for example, the isobaric labelling of peptides and label-free analysis. External calibration standards are useful for other applications.
Specifications
Ultimate 3000 HPLC system
- Flow Rate Range: Up to 10 mL/min
- Maximum Pressure: Up to 620 bar (9,000 psi)
Mass Spectrometer
- Mass Range: 50 – 6,000 m/z
- Resolution: Up to 240,000 at m/z 200
Applications
- Bottom-up proteomics
- Lipidomics
Instrument location
Bioanalytical Mass Spectrometry Facility
Room 2012, Level 2 South
Biosciences Precinct (E26)
UNSW Sydney, NSW 2033
Phone: 02 9385 1717
Email: bmsf@unsw.edu.au
Dr Russell Pickford
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Phone
02 9385 2952 -
Mobile
410 761 718 -
Email
l.adler@unsw.edu.au
Dr Martin Bucknall
-
Phone
02 9385 4707 -
Email
m.bucknall@unsw.edu.au
Parent facility
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