Thermo QExactive Plus
Description
The QE+ is a hybrid instrument that incorporates both quadrupole and Orbitrap mass analysers; both 1D and 2D nano liquid chromatography (LC) separations can be interfaced to this instrument. This platform allows the benefits of Orbitrap mass analysis to be exploited in highly sensitive experiments, as well as the rapid mass selection capabilities of quadrupoles.
Detailed insights into analyte molecule structural characteristics can be obtained via tandem mass spectrometry (MS/MS) experiments. Analyses are frequently performed upon complex peptide mixtures generated via the enzymatic or chemical hydrolysis of proteins, allowing confident protein identifications to be readily made at attomole levels. Quantitative analyses are possible via, for example, the isobaric labelling of peptides and label-free analysis.
Specifications
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- Low flow liquid chromatography system
- Flow rate range: 50 nL/min to 50 µL/min
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- Resolving Power: Up to 140,000 (FWHM) at m/z 200
- Mass Range: 50 to 6,000 m/z
- Mass Accuracy: <1 ppm with internal calibration
- Ion Source: Ion Max API source with H-ESI II probe
Applications
- Bottom-up proteomics
- Quantitative proteomics
- Post-Translational Modification (PTM) studies
Instrument location
Bioanalytical Mass Spectrometry Facility
Room 2012, Level 2 South
Biosciences Precinct (E26)
UNSW Sydney, NSW 2033
Phone: 02 9385 1717
Email: bmsf@unsw.edu.au
A/Prof Mark Raftery
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Phone
02 9385 1892 -
Email
m.raftery@unsw.edu.au
Dr Ling Zhong
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Phone
02 9385 2717 -
Email
l.zhong@unsw.edu.au
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