Wyatt Asymmetric Field Flow Fractionation (AF4)
Description
Field flow fractionation (FFF) is a rapid but gentle separation technique similar to size exclusion chromatography that uses a membrane or hollow-fibre instead of a column. In this technique, a perpendicular flow is used to separate molecules on either a membrane (AF4) or a hollow-fibre (HF5).
The FFF system in the lower campus laboratory uses a Shimadzu Prominence HPLC system to deliver the solvent and inject samples. A Wyatt Dualtec module is used to control the different flows across the membrane or hollow-fibre. The system also has various detectors that provide information about the size and mass of molecules.
Specifications
Chromatography
- Shimadzu Prominence HPLC
Detectors
- Ultraviolet (UV)
- Refractive Index (RI)
- Dynamic and Static Light Scattering
Applications
The Wyatt AF4 is used for the separation and analysis of:
- Water-soluble nano particles
- Micelles
- Proteins
- Poiymers
Instrument location
Bioanalytical Mass Spectrometry Facility
BMSF
June Griffith Building (F10)
UNSW Sydney, NSW 2033
Phone: 02 9385 1717
Email: bmsf@unsw.edu.au
Mr Lewis Adler
-
Phone
02 9385 7739 -
Email
l.adler@unsw.edu.au
Dr Chowdhury Sarowar
-
Phone
02 9348 1699 -
Email
c.h.sarowar@unsw.edu.au
Parent facility
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