The Bruker InViSPIM Lattice Pro - FLIM
Gaussian lightsheet beam profile for Illumination, changes in fluorescence lifetime can be captured at high speed, providing detailed and quantitative insights into various biological processes
Description
The Bruker InViSPIM Lattice Pro inverted lightsheet microscope with FLIM capabilities allows for the collection of quantitative data on the fluorescence lifetime of molecules. Using a Gaussian lightsheet beam profile for Illumination, changes in fluorescence lifetime can be captured at high speed, providing detailed and quantitative insights into various biological processes. It is suitable for studies of protein-protein interactions, intracellular environments, and metabolic states.
Specifications
- Illumination : 28.6x / 0.7 NA
- Detection Nikon: 25x / 1.1 NA
- Effective Magnification: 31.3x / 62.5x
- Field of View: 420 µm / 210 µm Pixel Size 208 nm / 104 nm
- Optical Resolution: 255 nm
- Detectors:
- Camera 1: pco.flim Camera
- Camera 2: sCMOS cameras Hamamatsu Orca Flash 4.0 V3
- Lasers:
- Lightsheet: 405nm, 445nm, 488 nm, 561 nm, 594 nm, 647 nm FLIM: 488 nm - Detectors:
- Camera 1: Hamamatsu ORCA-Fusion
- Camera 2: Hamamatsu ORCA-Fusion Filters wheel short Filter wheel long
Applications
- Fluorescence Live cell imaging
- Fluorescence lifetime imaging
- Developmental biology
- Cellular Dynamics
- Optical sectioning
- Cancer Research
Instrument location
Katharina Gaus Light Microscopy Facility
Level 2
Biosciences (E26)
UNSW Sydney, NSW 2033
Phone: 02 9065 5306
Email: KGlmf@unsw.edu.au
Dr Alex Macmillan
Head of KGLMF
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Email
alex.macmillan@unsw.edu.au
Dr Michael Carnell
Senior Lecturer
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Email
m.carnell@unsw.edu.au
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