BLItz

Analysis of protein interactions with minimal sample volume
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MWAC MSI lab instrument

Description

BLItz interferometry-based biosensor enables label-free, real-time analysis of protein interactions with minimal sample volume. This single-channel, manually-operated instrument and is ideally suited for simple, low volume experiments and can tolerate crude samples and nanoparticles.

Specifications

  • Ligand Sample (immobilized): 10 – 50 µg/ml
  • Analyte: 0.01 – 100x KD (0.1 – 10x KD)
  • Analyte size: ≥10 kDa
  • Sample and Reagent volume: 4 μl sample volume, 250 μl reagent volume
    Disposable biosensors (sensor regeneration not required)
  • KD range: nM to mM

Applications

  • Kinetic binding: ka, kd
  • Equilibrium binding: KD
  • Macromolecule binding
  • Macromolecule quantification in crude or purified sample

Related instruments

Instrument location

Molecular Surface Interaction Laboratory

Room 2002, Level 2
BABS (E26)
UNSW Sydney, NSW 2033

Email: msi@unsw.edu.au

Dr Xiaojing Huang

Senior Technical Officer
  • Send email envelope icon
    Email
    xiaojing.huang@unsw.edu.au

Parent facility

Molecular Surfaces Interaction Laboratory (MSI)

Our Molecular Surface Interaction Laboratory houses equipment that analyses interactions on the nanoscale. This includes interactions between proteins, peptides, antibodies, small molecules, carbohydrates, cells and materials.

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