Monolith NT.115 – Red/Blue
Biophysical technique that exploits the motion change of molecules in a microscopic temperature gradient during binding events
Description
Monolith NT.115 uses microscale thermophoresis (MST), which is a biophysical technique that exploits the motion change of molecules in a microscopic temperature gradient during binding events. Monolith NT.115 measures the strength of the interaction between two molecules by detecting variations in the fluorescence signal.
Specifications
- Ligand Sample (fluorescent): ≥ 5 nM (recommend 20 nM)
- Analyte: 0.01 – 100x KD (0.1 – 10x KD)
- Immobilization free, in-solution measurements
- Wide size range for interactants (from 10 Da to 10 MDa complexes)
- Temperature control: 22-45°C
- Sample volumn: < 10 μl per concentration
- KD range: nM to mM
- Nano RED detector
- Excitation wavelength: 600 – 650 nm
- Sample fluorophores: NT650, AlexaFluor647, Cy5
- Nano BLUE detector
- Excitation wavelength: 460 - 490 nm
- Sample fluorophores: fluorescein, GFP, AlexaFluor488
Applications
- Equilibrium binding: KD
- Proteins and molecules aggregation check
- Competitive assay
Instrument location
Molecular Surface Interaction Laboratory
Room 2002, Level 2
BABS (E26)
UNSW Sydney, NSW 2033
Email: msi@unsw.edu.au
Dr Xiaojing Huang
Senior Technical Officer
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Email
xiaojing.huang@unsw.edu.au
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