Chirascan Circular Dichroism Spectropolarimeter
Description
Circular dichroism (CD) measures the difference in the absorbance of left-handed circularly polarised light (L-CPL) and right-handed circularly polarised light (R-CPL), occuring in molecules with chiral chromophores (light-absorbing groups).
It is primarily used to analyse the secondary structure or conformation of macromolecules, particularly proteins.
Since secondary structure is sensitive to its environment, e.g. temperature or pH, CD can be used to observe how these structures change under different environmental conditions or when interacting with other molecules.
Specifications
- Cuvette holder options: 1cm, 1 mm, 0.5 mm, 0.2 mm and 0.1 mm.
- Variable temperature accessory for 1 cuvette or 4 cuvettes.
Applications
- Analyzes macromolecule or aggregate conformation in solution.
- Monitors secondary structure changes from binding events.
- Measures structural changes from temperature or chemical denaturation (e.g., oligonucleotide melting).
Instrument location
Structural Biology Facility
Mark Wainwright Analytical Centre
Office 320D, Level 3 West,
Biosciences North D26
UNSW Kensington NSW 2033
Phone: 02 9385 4587
Email: sbf@unsw.edu.au
Access
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SBF Email
sbf@unsw.edu.au
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