Nanotemper Prometheus NT.48

Monitors the intrinsic fluorescence signal of a protein sample
Personalise
MWAC structural biology facility lab instrument

Description

The Nanotemper Prometheus is used to test protein stability and protein aggregation by either chemical or thermal denaturation. The instrument monitors the intrinsic fluorescence signal of a protein sample as a measure of its folding state. A ratio metric measurement of the fluorescent signal is plotted against either increasing concentration of a chemical denaturant or increasing temperature, to determine the Tm of a protein. Results are available within minutes.

Specifications

  • Samples: up to 48 at a time 
  • Sample volume: minimum 10 µl 
  • Sample concentration: minimum 5 µg/ml

Applications

  • Protein stability 
  • Protein aggregation 
  • Chemical and thermal denaturation 
  • Buffer screening

Instrument location

Structural Biology Facility

Mark Wainwright Analytical Centre
Office 320D, Level 3 West, 
Biosciences North D26
UNSW Kensington NSW 2033 

Phone: 02 9385 4587 
Email: sbf@unsw.edu.au

Access

To discuss training or how your project could benefit from using our instrument, please contact:
  • Send email envelope icon
    SBF Email
    sbf@unsw.edu.au

Explore more instruments, facilities & services

Our infrastructure and expertise are accessible to UNSW students and staff, external researchers, government, and industry.