Back to

Associate Professor Alastair Stewart

Conjoint Senior Lecturer

Medicine & Health

School of Clinical Medicine

Location

405 Liverpool Street, Darlinghurst, NSW 2010

Book Chapters | 2020

Sobti M; Ishmukhametov R; Stewart AG, 2020, 'ATP Synthase: Expression, Purification, and Function', in Methods in Molecular Biology, pp. 73 - 84, http://dx.doi.org/10.1007/978-1-4939-9869-2_5
Journal articles | 2025

Furlong EJ; Reininger-Chatzigiannakis IBP; Zeng YC; Brown SHJ; Sobti M; Stewart AG, 2025, 'The molecular structure of an axle-less F1-ATPase', Biochimica et Biophysica Acta - Bioenergetics, 1866, http://dx.doi.org/10.1016/j.bbabio.2024.149521

Journal articles | 2025

Mazigi O; Langley DB; Henry JY; Burnett DL; Sobti M; Walker GJ; Rouet R; Balachandran H; Lenthall H; Jackson J; Ubiparipovic S; Schofield P; Brown SHJ; Schulz SR; Hoffmann M; Pöhlmann S; Post J; Martinello M; Ahlenstiel G; Kelleher A; Rawlinson WD; Turville SG; Bull RA; Stewart AG; Jäck HM; Goodnow CC; Christ D, 2025, 'Affinity maturation endows potent activity onto class 6 SARS-CoV-2 broadly neutralizing antibodies', Proceedings of the National Academy of Sciences of the United States of America, 122, http://dx.doi.org/10.1073/pnas.2417544121

Journal articles | 2024

Chen W; Zeng YC; Achinger-Kawecka J; Campbell E; Jones AK; Stewart AG; Khoury A; Clark SJ, 2024, 'Machine learning enables pan-cancer identification of mutational hotspots at persistent CTCF binding sites', Nucleic Acids Research, 52, pp. 8086 - 8099, http://dx.doi.org/10.1093/nar/gkae530

Journal articles | 2024

Lau CHY; Flood E; Hunter MJ; Williams-Noonan BJ; Corbett KM; Ng CA; Bouwer JC; Stewart AG; Perozo E; Allen TW; Vandenberg JI, 2024, 'Potassium dependent structural changes in the selectivity filter of HERG potassium channels', Nature Communications, 15, http://dx.doi.org/10.1038/s41467-024-51208-w

Journal articles | 2024

Sobti M; Ueno H; Brown SHJ; Noji H; Stewart AG, 2024, 'The series of conformational states adopted by rotorless F1-ATPase during its hydrolysis cycle', Structure, 32, pp. 393 - 399.e3, http://dx.doi.org/10.1016/j.str.2023.12.014

Journal articles | 2024

Stewart AG, 2024, 'Structural studies on Bacillus PS3 F₁-ATPase', BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1865, pp. 27 - 28, http://dx.doi.org/10.1016/j.bbabio.2024.149172

Journal articles | 2023

Lau C; Flood E; Hunter M; Corbett K; Ng C; Bouwer J; Stewart A; Perozo E; Allen TW; Vandenberg J, 2023, 'Structural basis of rapid inactivation of HERG potassium channels', BIOPHYSICAL JOURNAL, 122, pp. 446A - 446A, http://dx.doi.org/10.1016/j.bpj.2022.11.2409

Journal articles | 2023

Rouet R; Henry JY; Johansen MD; Sobti M; Balachandran H; Langley DB; Walker GJ; Lenthall H; Jackson J; Ubiparipovic S; Mazigi O; Schofield P; Burnett DL; Brown SHJ; Martinello M; Hudson B; Gilroy N; Post JJ; Kelleher A; Jäck HM; Goodnow CC; Turville SG; Rawlinson WD; Bull RA; Stewart AG; Hansbro PM; Christ D, 2023, 'Broadly neutralizing SARS-CoV-2 antibodies through epitope-based selection from convalescent patients', Nature Communications, 14, http://dx.doi.org/10.1038/s41467-023-36295-5

Journal articles | 2023

Sobti M; Mead BJ; Stewart AG; Igreja C; Christie M, 2023, 'Molecular basis for GIGYF–TNRC6 complex assembly', RNA, 29, pp. 724 - 734, http://dx.doi.org/10.1261/rna.079596.123

Journal articles | 2023

Sobti M; Zeng YC; Walshe JL; Brown SHJ; Ishmukhametov R; Stewart AG, 2023, 'Changes within the central stalk of E. coli F1Fo ATP synthase observed after addition of ATP', Communications Biology, 6, http://dx.doi.org/10.1038/s42003-023-04414-z

Journal articles | 2023

Zeng YC; Sobti M; Quinn A; Smith NJ; Brown SHJ; Vandenberg JI; Ryan RM; O’Mara ML; Stewart AG, 2023, 'Structural basis of promiscuous substrate transport by Organic Cation Transporter 1', Nature Communications, 14, http://dx.doi.org/10.1038/s41467-023-42086-9

Journal articles | 2022

Stewart AG; Sobti M; Ueno H; Noji H, 2022, 'The rotary catalytic cycle of a thermophilic F-1-ATPase', BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1863, pp. 60 - 60, http://dx.doi.org/10.1016/j.bbabio.2022.148772

Journal articles | 2021

Burnett DL; Jackson KJL; Langley DB; Aggrawal A; Stella AO; Johansen MD; Balachandran H; Lenthall H; Rouet R; Walker G; Saunders BM; Singh M; Li H; Henry JY; Jackson J; Stewart AG; Witthauer F; Spence MA; Hansbro NG; Jackson C; Schofield P; Milthorpe C; Martinello M; Schulz SR; Roth E; Kelleher A; Emery S; Britton WJ; Rawlinson WD; Karl R; Schäfer S; Winkler TH; Brink R; Bull RA; Hansbro PM; Jäck HM; Turville S; Christ D; Goodnow CC, 2021, 'Immunizations with diverse sarbecovirus receptor-binding domains elicit SARS-CoV-2 neutralizing antibodies against a conserved site of vulnerability', Immunity, 54, pp. 2908 - 2921.e6, http://dx.doi.org/10.1016/j.immuni.2021.10.019

Journal articles | 2021

Chen I; Pant S; Wu Q; Cater RJ; Sobti M; Vandenberg RJ; Stewart A; Font J; Tajkhorshid E; Ryan RM, 2021, 'Glutamate Transporters (EAATS) Contain a Conserved Chloride Channel with Two Hydrophobic Gates', BIOPHYSICAL JOURNAL, 120, pp. 104A - 105A, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000629601400503&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1

Journal articles | 2021

Chen I; Pant S; Wu Q; Cater RJ; Sobti M; Vandenberg RJ; Stewart AG; Tajkhorshid E; Font J; Ryan RM, 2021, 'Glutamate transporters have a chloride channel with two hydrophobic gates', Nature, 591, pp. 327 - 331, http://dx.doi.org/10.1038/s41586-021-03240-9

Journal articles | 2021

Ngo T; Ilatovskiy AV; Stewart AG; Coleman JLJ; McRobb FM; Riek RP; Graham RM; Abagyan R; Kufareva I; Smith NJ, 2021, 'Retraction Note: Orphan receptor ligand discovery by pickpocketing pharmacological neighbors (Nature Chemical Biology, (2017), 13, 2, (235-242), 10.1038/nchembio.2266)', Nature Chemical Biology, 17, pp. 501, http://dx.doi.org/10.1038/s41589-021-00746-1

Journal articles | 2021

Pant S; Chen I; Wu Q; Cater R; Sobti M; Vandenberg RJ; Stewart AG; Font J; Ryan RM; Tajkhorshid E, 2021, 'A Novel Chloride Conducting Conformation in Human Glutamate Transporters', BIOPHYSICAL JOURNAL, 120, pp. 171A - 171A, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000629601401081&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1

Journal articles | 2021

Rouet R; Mazigi O; Walker GJ; Langley DB; Sobti M; Schofield P; Lenthall H; Jackson J; Ubiparipovic S; Henry JY; Abayasingam A; Burnett D; Kelleher A; Brink R; Bull RA; Turville S; Stewart AG; Goodnow CC; Rawlinson WD; Christ D, 2021, 'Potent SARS-CoV-2 binding and neutralization through maturation of iconic SARS-CoV-1 antibodies', mAbs, 13, http://dx.doi.org/10.1080/19420862.2021.1922134

Journal articles | 2021

Sobti M; Mead BJ; Igreja C; Stewart AG; Christie M, 2021, 'Molecular basis for GIGYF-TNRC6 complex assembly in miRNA-mediated translational repression', , http://dx.doi.org/10.1101/2021.08.20.457040

Journal articles | 2021

Sobti M; Ueno H; Noji H; Stewart AG, 2021, 'The six steps of the complete F1-ATPase rotary catalytic cycle', Nature Communications, 12, http://dx.doi.org/10.1038/s41467-021-25029-0

Journal articles | 2021

Zeng YC; Sobti M; Stewart AG, 2021, 'Structural analysis of the Sulfolobus solfataricus TF55β chaperonin by cryo-electron microscopy', Acta Crystallographica Section F: Structural Biology Communications, 77, pp. 79 - 84, http://dx.doi.org/10.1107/S2053230X21002223

Journal articles | 2020

Rouet R; Mazigi O; Walker G; Langley D; Sobti M; Schofield P; Lenthall H; Jackson J; Ubiparipovic S; Henry J; Abayasingam A; Burnett D; Kelleher A; Brink R; Bull R; Turville S; Stewart A; Goodnow C; Rawlinson W; Christ D, 2020, 'Potent SARS-CoV-2 binding and neutralization through maturation of iconic SARS-CoV-1 antibodies', , http://dx.doi.org/10.1101/2020.12.14.422791

Journal articles | 2020

Sobti M; Walshe J; Zeng Y; Ishmukhametov R; Stewart A, 2020, 'Subunit epsilon ofE. coliF₁F_oATP synthase attenuates enzyme activity by modulating central stalk flexibility', , http://dx.doi.org/10.1101/2020.09.30.320408

Journal articles | 2020

Sobti M; Walshe JL; Wu D; Ishmukhametov R; Zeng YC; Robinson CV; Berry RM; Stewart AG, 2020, 'Cryo-EM structures provide insight into how E. coli F1Fo ATP synthase accommodates symmetry mismatch', Nature Communications, 11, http://dx.doi.org/10.1038/s41467-020-16387-2

Journal articles | 2020

Zeng YC; Sobti M; Stewart A, 2020, 'Structural analysis ofSulfolobus solfataricusTF55β chaperonin in open and filamentous states', , http://dx.doi.org/10.1101/2020.01.13.905216

Journal articles | 2019

Sobti M; Ishmukhametov R; Bouwer JC; Ayer A; Suarna C; Smith NJ; Christie M; Stocker R; Duncan TM; Stewart AG, 2019, 'Cryo-EM reveals distinct conformations of E. coli ATP synthase on exposure to ATP', eLife, 8, http://dx.doi.org/10.7554/eLife.43864

Journal articles | 2019

Sobti M; Walshe J; Ishmukhametov R; Stewart A, 2019, 'Visualizing movements inE. coliF₁F_oATP synthase indicates how the F₁and F_omotors are coupled', , http://dx.doi.org/10.1101/622084

Journal articles | 2018

Berger J; Berger S; Li M; Jacoby AS; Arner A; Bavi N; Stewart AG; Currie PD, 2018, 'In Vivo Function of the Chaperonin TRiC in α-Actin Folding during Sarcomere Assembly', Cell Reports, 22, pp. 313 - 322, http://dx.doi.org/10.1016/j.celrep.2017.12.069

Journal articles | 2018

Chorev DS; Baker LA; Wu D; Beilsten-Edmands V; Rouse SL; Zeev-Ben-Mordehai T; Jiko C; Samsudin F; Gerle C; Khalid S; Stewart AG; Matthews SJ; Grünewald K; Robinson CV, 2018, 'Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry', Science, 362, pp. 829 - 834, http://dx.doi.org/10.1126/science.aau0976

Journal articles | 2018

Lau C; Hunter MJ; Stewart A; Perozo E; Vandenberg JI, 2018, 'Never at rest: insights into the conformational dynamics of ion channels from cryo-electron microscopy', Journal of Physiology, 596, pp. 1107 - 1119, http://dx.doi.org/10.1113/JP274888

Journal articles | 2017

Chaston JJ; Stewart AG; Christie M, 2017, 'Structural characterisation of TNRC6A nuclear localisation signal in complex with importin-alpha', PLoS ONE, 12, pp. e0183587, http://dx.doi.org/10.1371/journal.pone.0183587

Journal articles | 2017

Davies RB; Smits C; Wong ASW; Stock D; Christie M; Sandin S; Stewart AG, 2017, 'Cryo-EM analysis of a domain antibody bound rotary ATPase complex', Journal of Structural Biology, 197, pp. 350 - 353, http://dx.doi.org/10.1016/j.jsb.2017.01.002

Journal articles | 2017

Ngo T; Ilatovskiy AV; Stewart AG; Coleman JLJ; McRobb FM; Riek RP; Graham RM; Abagyan R; Kufareva I; Smith NJ, 2017, 'Orphan receptor ligand discovery by pickpocketing pharmacological neighbors', Nature Chemical Biology, 13, pp. 235 - 242, http://dx.doi.org/10.1038/nchembio.2266

Journal articles | 2016

Al-Zyoud WA; Hynson RMG; Ganuelas LA; Coster ACF; Duff AP; Baker MAB; Stewart AG; Giannoulatou E; Ho JWK; Gaus K; Liu D; Lee LK; Böcking T; Baker M, 2016, 'Binding of transcription factor GabR to DNA requires recognition of DNA shape at a location distinct from its cognate binding site', Nucleic Acids Research, 44, pp. 1411 - 1420, http://dx.doi.org/10.1093/nar/gkv1466

Journal articles | 2016

Chaston JJ; Smits C; Aragão D; Wong ASW; Ahsan B; Sandin S; Molugu SK; Molugu SK; Bernal RA; Stock D; Stewart AG, 2016, 'Structural and Functional Insights into the Evolution and Stress Adaptation of Type II Chaperonins', Structure, 24, pp. 364 - 374, http://dx.doi.org/10.1016/j.str.2015.12.016

Journal articles | 2016

Christie M; Chang CW; Róna G; Smith KM; Stewart AG; Takeda AAS; Fontes MRM; Stewart M; Vértessy BG; Forwood JK; Kobe B, 2016, 'Structural Biology and Regulation of Protein Import into the Nucleus', Journal of Molecular Biology, 428, pp. 2060 - 2090, http://dx.doi.org/10.1016/j.jmb.2015.10.023

Journal articles | 2016

Christie M; Chang CW; Róna G; Smith KM; Stewart AG; Takeda AAS; Fontes MRM; Stewart M; Vértessy BG; Forwood JK; Kobe B, 2016, 'Structural Biology and Regulation of Protein Import into the Nucleus', Journal of molecular biology, 428, pp. 2060 - 2090, http://dx.doi.org/10.1016/j.jmb.2015.10.023

Journal articles | 2016

Sobti M; Smits C; Wong ASW; Ishmukhametov R; Stock D; Sandin S; Stewart AG, 2016, 'Cryo-EM structures of the autoinhibited E. coli ATP synthase in three rotational states', eLife, 5, pp. e21598, http://dx.doi.org/10.7554/eLife.21598

Journal articles | 2014

Stewart AG; Laming EM; Sobti M; Stock D, 2014, 'Rotary ATPases-dynamic molecular machines', Current Opinion in Structural Biology, 25, pp. 40 - 48, http://dx.doi.org/10.1016/j.sbi.2013.11.013

Journal articles | 2014

Stewart AG, 2014, 'The molecular v brake', Journal of Molecular Biology, 426, pp. 273 - 274, http://dx.doi.org/10.1016/j.jmb.2013.10.003

Journal articles | 2014

Zhou M; Politis A; Davies RB; Liko I; Wu KJ; Stewart AG; Stock D; Robinson CV, 2014, 'Ion mobility-mass spectrometry of a rotary ATPase reveals ATP-induced reduction in conformational flexibility', Nature Chemistry, 6, pp. 208 - 215, http://dx.doi.org/10.1038/nchem.1868

Journal articles | 2013

Stewart AG; Sobti M; Harvey RP; Stock D, 2013, 'Rotary ATPases: models, machine elements and technical specifications.', Bioarchitecture, 3, pp. 2 - 12, http://dx.doi.org/10.4161/bioa.23301

Journal articles | 2012

Stewart A; Lee L; Donohoe M; Chaston J; Stock D, 2012, 'The dynamic stator stalk of rotary ATPases', PROTEIN SCIENCE, 21, pp. 73 - 73, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000307019800060&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1

Journal articles | 2012

Stewart AG; Lee LK; Donohoe M; Chaston JJ; Stock D, 2012, 'The dynamic stator stalk of rotary ATPases', Nature communications, 3, http://dx.doi.org/10.1038/ncomms1693

Journal articles | 2012

Stock D; Stewart GS, 2012, 'Priming a Molecular Motor for Disassembly', Structure, 20, pp. 1799 - 1800, http://dx.doi.org/10.1016/j.str.2012.10.003

Journal articles | 2010

Lee LK; Stewart AG; Donohoe M; Bernal RA; Stock D, 2010, 'The Structure of the peripheral stalk of Thermus thermophilus H+-ATPase/Synthase', Nature Structural and Molecular Biology, 17, pp. 373 - 378, http://dx.doi.org/10.1038/nsmb.1761
Preprints | 2025

Suzuki AK; Furukawa R; Sobti M; Brown SHJ; Stewart AG; Akanuma S; Ueno H; Noji H, 2025, Functional and Structural Characterization of F₁-ATPase with common ancestral core domains in stator ring, http://dx.doi.org/10.1101/2025.04.30.651372

Preprints | 2024

Furlong EJ; Reininger-Chatzigiannakis I-BP; Zeng YC; Brown SHJ; Sobti M; Stewart AG, 2024, The molecular structure of an axle-less F1-ATPase., http://dx.doi.org/10.1101/2024.08.08.607276

Conference Abstracts | 2024

Stewart AG, 2024, 'Structural studies on Bacillus PS3 F₁-ATPase', in BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, ELSEVIER, AUSTRIA, Innsbruck, Vol. 1865, pp. 27 - 28, presented at 22nd European Bioenergetics Conference (EBEC), AUSTRIA, Innsbruck, 26 August 2024 - 31 August 2024, http://dx.doi.org/10.1016/j.bbabio.2024.149172

Conference Abstracts | 2023

Lau C; Flood E; Hunter M; Corbett K; Ng C; Bouwer J; Stewart A; Perozo E; Allen TW; Vandenberg J, 2023, 'Structural basis of rapid inactivation of HERG potassium channels', in BIOPHYSICAL JOURNAL, CELL PRESS, Vol. 122, pp. 446A - 446A, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000989629702422&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1

Preprints | 2023

Lau CHY; Flood E; Hunter MJ; Williams-Noonan BJ; Corbett KM; Ng C-A; Bouwer JC; Stewart AG; Perozo E; Allen TW; Vandenberg JI, 2023, Potassium dependent structural changes in the selectivity filter of HERG potassium channels, http://dx.doi.org/10.1101/2023.12.14.571769

Preprints | 2022

Rouet R; Henry J; Johansen M; Sobti M; Balachandran H; Langley D; Walker G; Lenthall H; Jackson J; Ubiparipovic S; Mazigi O; Schofield P; Burnett D; Brown S; Martinello M; Hudson B; Gilroy N; Post J; Kelleher A; Jäck H-M; Goodnow C; Turville S; Rawlinson W; Bull R; Stewart A; Hansbro P; Christ D, 2022, Broadly neutralizing SARS-CoV-2 antibodies through epitope-based selection from convalescent patients, http://dx.doi.org/10.1101/2022.10.19.512954

Conference Papers | 2021

Chen I; Wu Q; Pant S; Cater R; Sobti M; Vandenberg R; Stewart AG; Font J; Tajkhorshid E; Ryan RM, 2021, 'Glutamate transporters contain a conserved chloride channel with two hydrophobic gates', in BIOPHYSICAL REVIEWS, SPRINGERNATURE, pp. 1497 - 1497

Preprints | 2021

Vandenberg J; Lau C; Flood E; Hunter M; Ng C-A; Bouwer J; Stewart A; Perozo E; Allen T, 2021, Structural Basis for Rapid Voltage Dependent Inactivation of HERG Potassium Channels, http://dx.doi.org/10.21203/rs.3.rs-1105661/v1

Preprints | 2020

Chen I; Pant S; Wu Q; Cater R; Sobti M; Vandenberg R; Stewart AG; Tajkhorshid E; Font J; Ryan R, 2020, Glutamate transporters contain a conserved chloride channel with two hydrophobic gates, http://dx.doi.org/10.1101/2020.05.25.115360

Preprints | 2020

Sobti M; Ueno H; Noji H; Stewart AG, 2020, The six steps of the F₁-ATPase rotary catalytic cycle, http://dx.doi.org/10.1101/2020.12.15.422962

Follow

Associate Professor Alastair Stewart