Dr Khawar Siddiqui
Project Officer

Dr Khawar Siddiqui

PhD: (Biotechnology) Imperial College, London, UK

MSc: (Zoological Sciences) Peshawar University, Pakistan

BSc: (Biology, Chemistry, English) Sadiq-Egerton College, Bahawalpur, Pakistan

Science
School of Biotech & Biomolecular Science

Professional Experience

  • Nov 2019 - ongoing: School of Biotechnology & Biomolecular Sciences (BABS), University of New South Wales, Sydney. Project Officer
  • Aug 2014-aug 2019: Life Science Dept. King Fahd University of Petroleum and Minerals (KFUPM), Dhahran, Saudi Arabia. Associate Professor
  • Jan-2005 to July 2014: School of Biotechnology & Biomolecular Sciences (BABS), University of New South Wales, Sydney. Senior Research Associate
  • 15-8-2004 to 5-1-2005: Biosciences Dept, COMSATS Institute of Information Technology, Islamabad, Pakistan. Visiting Professor
  • Feb-2001 to Aug-2004: School of Biotechnology & Biomolecular Sciences, UNSW, Sydney, Australia. Senior Research Associate
  • 26-2-2001 - 4-6-2001: Biochemistry Dept, Sydney University, Sydney.Teaching Demonstration
  • 1999-2000: Protein Chemistry Division, Pharmaceuticals Research Centre, King Abdul-Asia City of Science and Technology, (SPIMACO), Riyadh, Saudi Arabia in collaboration with Orpegen Therapeutika, Germany Research Specialist
  • 1990-1999: National Institute for Biotechnology and Genetic Engineering, (NIBGE), Jhang Road, Faisalabad, Pakistan.Senior Scientific Officer
  • 1986-1990: Centre for  Biotechnology, Imperial College, London . PhD student

 

Research Contribution

My field of research involves protein chemistry and biocatalysis using enzymes. The information gained from the bioinformatic, kinetic and thermodynamic characterization of native, chemically and genetically modified enzymes is utilized for the elucidation of protein structure-function-stability relationship. I am currently working on Biochemical analysis in tissues, enzymes that are implicated in the production of glutathione and bioinformatics screening of plant molecules as the inhibitors for the main protease of COVID-19 virus. In addition I employed extremophilic enzymes  from Antarctica's cold adapted organisms in order to enhance their stability while keeping their high intrinsic activity. Conversely, I enhanced the activity of industrially important thermophilic protease at low temperatures by chemical modification. I apply native and improved enzymes for treating waste-waters as well as for cleaning fouled membranes. 

For purification and characterisation of proteins we employ a variety of biochemical and biophysical techniques such as chromatography, electrophoresis, UV-Vis spectrometry, spectropolarimetry, fluorimetry and calorimetry (differential scanning and isothermal titration calorimetry). In addition we employ methods such as small-angle X-ray scattering (SAXS), mass spectrometry and X-ray diffraction crystallography in collaboration with other groups.

  • Cited among Top Scientists of the Country published by Pakistan Council of Science & Technology - February 2001
  • Invited review in many high impact journals
  • Co-edit a book entitled "Protein adaption in extremophiles" published by Nova Science Publisher - New York, USA (2008)
  • Best Teacher's Certificate (MPhil Biotechnology) awarded by National Institute of Biotechnology and Genetic Engineering - Faisalabad, Pakistan (1995-1998)
  • Written four text book chapters with other colleagues
  • Reviewer for many biochemical and biotechnology journals of international repute
  • Cited on the cover of Uniken - UNSW, Sydney, Australia
  • PhD Scholarship awarded by Ministry of Science & Technology - Pakistan (1986)
Phone
+61 0470538639
Location
Room 420, Level 4, BABS (Office) Room 403, Level 4, BABS (Lab)
  • Journal articles | 2016
    Najnin T; Siddiqui KS; Taha ; Elkaid N; Kornfeld G; Curmi PMG; Cavicchioli R, 2016, 'Characterization of a temperature-responsive two component regulatory system from the Antarctic archaeon, Methanococcoides burtonii', Scientific Reports, vol. 6, http://dx.doi.org/10.1038/srep24278
    Journal articles | 2016
    Taha ; Siddiqui KS; Campanaro S; Najnin T; Deshpande N; Williams TJ; Aldrich-Wright J; Wilkins M; Curmi PMG; Cavicchioli R, 2016, 'Single TRAM domain RNA-binding proteins in Archaea: functional insight from Ctr3 from the Antarctic methanogen Methanococcoides burtonii', Environmental microbiology, vol. 18, pp. 2810 - 2824, http://dx.doi.org/10.1111/1462-2920.13229
    Journal articles | 2015
    Guerriero G; Hausman JF; Strauss J; Ertan H; Siddiqui KS, 2015, 'Destructuring plant biomass: Focus on fungal and extremophilic cell wall hydrolases', Plant Science, vol. 234, pp. 180 - 193, http://dx.doi.org/10.1016/j.plantsci.2015.02.010
    Journal articles | 2014
    Siddiqui KS; Ertan H; Charlton T; Poljakc A; Khaled AK; Yang X; Marshall G; Cavicchioli R, 2014, 'Versatile peroxidase degradation of humic substances: Use of isothermal titration calorimetry to assess kinetics, and applications to industrial wastes', Journal of Biotechnology, vol. 178, pp. 1 - 11, http://dx.doi.org/10.1016/j.jbiotec.2014.03.002
    Journal articles | 2012
    Muenchhoff J; Siddiqui KS; Neilan BA, 2012, 'Identification of two residues essential for the stringent substrate specificity and active site stability of the prokaryotic l -arginine:glycine amidinotransferase CyrA', FEBS Journal, vol. 279, pp. 805 - 815, http://dx.doi.org/10.1111/j.1742-4658.2012.08472.x
    Journal articles | 2009
    Siddiqui KS; Parkin DM; Curmi PM; De Francisci D; Poljak A; Barrow KD; Noble M; Trewhella J; Cavicchioli R, 2009, 'A novel approach for enhancing the catalytic efficiency of a protease at low temperature: Reduction in substrate inhibition by chemical modification', Biotechnology and Bioengineering, vol. 103, pp. 676 - 686, http://dx.doi.org/10.1002/bit.22300
    Journal articles | 2006
    Siddiqui KS; Poljak A; Guilhaus M; De Francisci D; Curmi PM; Feller G; D Amico S; Gerday C; Uversky V; Cavicchioli R, 2006, 'Role of lysine versus arginine in enzyme cold-adaptation: Modifying lysine to homo-arginine stabilizes the cold-adapted alpha-amylase from Pseudoalteramonas haloplanktis', Proteins - Structure Function and Bioinformatics, vol. 64, pp. 486 - 501, http://dx.doi.org/10.1002/prot.20989

My Teaching

Biology for Engineers: Undergraduate level, Life Sciences Dept. KFUPM, Saudi Arabia

Techniques in Life Sciences: Master's level, Life Sciences Dept., KFUPM, Saudi Arabia

Protein Bioinformatics: Undergraduate level, Biology Dept., COMSATS Institute of Technology, Islamabad, Pakistan.

Techniques in Biotechnology: MPhil level, National Institute of Biotechnology & Genetic Engineering, Faisalabad, Pakistan

Enzymology: MPhil level, National Institute of Biotechnology & Genetic Engineering, Faisalabad, Pakistan