Associate Professor Till Boecking
Associate Professor

Associate Professor Till Boecking

Medicine & Health
School of Clinical Medicine

Till Böcking leads the Molecular Machines Group in the EMBL Australia Node for Single Molecule Science. Originally trained in biochemistry at the University of Bonn in Germany, he crossed disciplines and completed a PhD in Biophysics at UNSW with Hans Coster and Kevin Barrow in 2004. During his doctoral and subsequent postdoctoral research with Justin Gooding and Michael Gal, he developed self-assembly chemistries to assemble biomolecules on surfaces. This work lead to the development of biosensors and the discovery of fundamental principles that govern the performance of these devices. In 2006 he joined the group of Tomas Kirchhausen at Harvard Medical School to elucidate the mechanism of the chaperone-mediated disassembly of the protein coat surrounding endocytic vesicles. Till was awarded a Cross-Disciplinary Fellowship of the Human Frontier Science Program in 2007. Since returning to Australia, Till leads independent research focused on understanding biological processes at the molecular level using approaches from the physical sciences. In particular his team uses single-molecule techniques to resolve mechanistsic questions inaccessible with traditional approaches. 

Phone
+61 2 9385 1179
Location
Lowy Cancer Centre Building, Level 3
  • Book Chapters | 2018
    Böcking T; Upadhyayula S; Rapoport I; Capraro BR; Kirchhausen T, 2018, 'Reconstitution of clathrin coat disassembly for fluorescence microscopy and single-molecule analysis', in Clathrin-Mediated Endocytosis. Methods in Molecular Biology, Humana Press, New York, NY, pp. 121 - 146, http://dx.doi.org/10.1007/978-1-4939-8719-1_10
  • Journal articles | 2022
    Brown JWP; Alford RG; Walsh JC; Spinney RE; Xu SY; Hertel S; Berengut JF; Spenkelink LM; Van Oijen AM; Böcking T; Morris RG; Lee LK, 2022, 'Rapid Exchange of Stably Bound Protein and DNA Cargo on a DNA Origami Receptor', ACS Nano, vol. 16, pp. 6455 - 6467, http://dx.doi.org/10.1021/acsnano.2c00699
    Journal articles | 2022
    Johnstone BA; Joseph R; Christie MP; Morton CJ; McGuiness C; Walsh JC; Böcking T; Tweten RK; Parker MW, 2022, 'Cholesterol-dependent cytolysins: The outstanding questions', IUBMB Life, http://dx.doi.org/10.1002/iub.2661
    Journal articles | 2022
    Longatte G; Lisi F; Bakthavathsalam P; Böcking T; Gaus K; Tilley RD; Gooding JJ, 2022, 'Biomolecular Binding under Confinement: Statistical Predictions of Steric Influence in Absence of Long-Distance Interactions', ChemPhysChem, vol. 23, http://dx.doi.org/10.1002/cphc.202100765
    Journal articles | 2022
    Longatte G; Lisi F; Chen X; Walsh J; Wang W; Ariotti N; Boecking T; Gaus K; Gooding JJ, 2022, 'Statistical predictions on the encapsulation of single molecule binding pairs into sized-dispersed nanocontainers', Physical Chemistry Chemical Physics, http://dx.doi.org/10.1039/d2cp03627d
    Journal articles | 2022
    Zuliani-Alvarez L; Govasli ML; Rasaiyaah J; Monit C; Perry SO; Sumner RP; McAlpine-Scott S; Dickson C; Rifat Faysal KM; Hilditch L; Miles RJ; Bibollet-Ruche F; Hahn BH; Boecking T; Pinotsis N; James LC; Jacques DA; Towers GJ, 2022, 'Evasion of cGAS and TRIM5 defines pandemic HIV', Nature Microbiology, vol. 7, pp. 1762 - 1776, http://dx.doi.org/10.1038/s41564-022-01247-0
    Journal articles | 2020
    Lau D; Walsh JC; Mousapasandi A; Ariotti N; Shah VB; Turville S; Jacques DA; Böcking T, 2020, 'Self-Assembly of Fluorescent HIV Capsid Spheres for Detection of Capsid Binders', Langmuir, vol. 36, pp. 3624 - 3632, http://dx.doi.org/10.1021/acs.langmuir.0c00103
    Journal articles | 2019
    Marquez CL; Lau D; Walsh J; Faysal KMR; Parker MW; Turville SG; Bocking T, 2019, 'Fluorescence Microscopy Assay to Measure HIV-1 Capsid Uncoating Kinetics in vitro', BIO-PROTOCOL, vol. 9, http://dx.doi.org/10.21769/BioProtoc.3297
    Journal articles | 2018
    Márquez CL; Lau D; Walsh J; Shah V; McGuinness C; Wong A; Aggarwal A; Parker MW; Jacques DA; Turville S; Böcking T, 2018, 'Kinetics of HIV-1 capsid uncoating revealed by single-molecule analysis', eLife, vol. 7, http://dx.doi.org/10.7554/eLife.34772
    Journal articles | 2016
    Al-Zyoud WA; Hynson RMG; Ganuelas LA; Coster ACF; Duff AP; Baker MAB; Stewart AG; Giannoulatou E; Ho JWK; Gaus K; Liu D; Lee LK; Böcking T, 2016, 'Binding of transcription factor GabR to DNA requires recognition of DNA shape at a location distinct from its cognate binding site', Nucleic Acids Research, vol. 44, pp. 1411 - 1420, http://dx.doi.org/10.1093/nar/gkv1466
    Journal articles | 2016
    Bonello TT; Janco M; Hook J; Byun A; Appaduray M; Dedova I; Hitchcock-DeGregori S; Hardeman EC; Stehn JR; Böcking T; Gunning PW, 2016, 'A small molecule inhibitor of tropomyosin dissociates actin binding from tropomyosin-directed regulation of actin dynamics', Scientific Reports, vol. 6, http://dx.doi.org/10.1038/srep19816
    Journal articles | 2016
    Janco M; Bonello TT; Byun A; Coster ACF; Lebhar H; Dedova I; Gunning PW; Böcking T, 2016, 'The impact of tropomyosins on actin filament assembly is isoform specific', BioArchitecture, vol. 6, pp. 61 - 75, http://dx.doi.org/10.1080/19490992.2016.1201619
    Journal articles | 2016
    Nicovich PR; Janco M; Sobey T; Gajwani M; Obeidy P; Whan R; Gaus K; Gunning PW; Coster ACF; Böcking T, 2016, 'Effect of surface chemistry on tropomyosin binding to actin filaments on surfaces', Cytoskeleton, vol. 73, pp. 729 - 738, http://dx.doi.org/10.1002/cm.21342
    Journal articles | 2015
    Pham S; Tabarin T; Garvey M; Pade C; Rossy J; Monaghan P; Hyatt A; Böcking T; Leis A; Gaus K; Mak J, 2015, 'Cryo-electron microscopy and single molecule fluorescent microscopy detect CD4 receptor induced HIV size expansion prior to cell entry', Virology, vol. 486, pp. 121 - 133, http://dx.doi.org/10.1016/j.virol.2015.09.006
    Journal articles | 2015
    Polling S; Ormsby AR; Wood RJ; Lee K; Shoubridge C; Hughes JN; Thomas PQ; Griffin MDW; Hill AF; Bowden Q; Böcking T; Hatters DM, 2015, 'Polyalanine expansions drive a shift into α-helical clusters without amyloid-fibril formation', Nature Structural and Molecular Biology, vol. 22, pp. 1008 - 1015, http://dx.doi.org/10.1038/nsmb.3127
    Journal articles | 2014
    Böcking T; Aguet F; Rapoport I; Banzhaf M; Yu A; Zeeh JC; Kirchhausen T, 2014, 'Key interactions for clathrin coat stability', Structure, vol. 22, pp. 819 - 829, http://dx.doi.org/10.1016/j.str.2014.04.002
    Journal articles | 2011
    Le saux G; Magenau AI; Boecking T; Gaus K; Gooding JJ, 2011, 'The Relative Importance of Topography and RGD Ligand Density for Endothelial Cell Adhesion', PLoS ONE, vol. 6, pp. e21869, http://dx.doi.org/10.1371/journal.pone.0021869
    Journal articles | 2011
    Le saux G; Magenau AI; Gunaratnam K; Kilian KA; Boecking T; Gooding JJ; Gaus K, 2011, 'Spacing of Integrin Ligands Influences Signal Transduction in Endothelial Cells', Biophysical Journal, vol. 101, pp. 764 - 773, http://dx.doi.org/10.1016/j.bpj.2011.06.064
    Journal articles | 2009
    Kilian KA; Lai LH; Magenau AI; Cartland SP; Boecking T; Di Girolamo N; Gal M; Gaus K; Gooding JJ, 2009, 'Smart tissue culture: in Situ monitoring of the activity of protease enzymes secreted from live cells using nanostructured photonic crystals', Nano Letters, vol. 9, pp. 2021 - 2025, http://dx.doi.org/10.1021/nl900283j
    Journal articles | 2008
    Ciampi S; Boecking T; Kilian KA; Harper JB; Gooding JJ, 2008, 'Click chemistry in mesoporous materials: Functionalization of porous silicon rugate filters', Langmuir, vol. 24, pp. 5888 - 5892, http://dx.doi.org/10.1021/la800435d
    Journal articles | 2007
    Ciampi S; Boecking T; Kilian KA; James M; Harper JB; Gooding JJ, 2007, 'Functionalization of acetylene-terminated monolayers on Si(100) surfaces: A click chemistry approach', Langmuir, vol. 23, pp. 9320 - 9329, http://dx.doi.org/10.1021/la701035g
  • Preprints | 2022
    Brown JWP; Alford R; Walsh J; Spinney R; Xu S; Hertel S; Berengut J; Spenkelink L; van Oijen A; Böcking T; Morris R; Lee L, 2022, Rapid exchange of stably bound protein and DNA cargo on a DNA origami receptor, http://dx.doi.org/10.1101/2022.01.16.476526

  • 2011-2014 ARC Future Fellowship
  • 2011-2014 NHMRC Career Development Award (honorary)
  • 2009 Young Investigator Award Lorne Protein Conference
  • Young Biophysicist Award of the Australian Society for Biophysics
  • 2007–2010 HFSP Cross-Disciplinary Fellowship (3 years)
  • 2007 Federation of the European Biochemical Societies (FEBS) Youth Travel Fund Grant
  • 2001–2003 UNSW Faculty of Science Scholarship
  • 2001–2003 Australian Institute for Nuclear Science and Engineering (AINSE) Postgraduate Award
  • 2000–2003 International Postgraduate Research Scholarship (IPRS)
  • 1996 Deutscher Akademischer Austauschdienst (DAAD) Scholarship

  • Australian Society for Biochemistry and Molecular Biology (ASBMB)
  • Australian Society for Biophysics (ASB)

My Research Supervision

PhD students: Chantal Marquez, Ilina Bareja, Derrick Lau, KM Rifat Faysal, Wang Peng, Conall McGuinness

Honours students: Shivani Shah

My Teaching

ANAT3212