Nikon N-STORM
Description
This is a total internal fluorescence (TIRF) allowing the imaging of molecules at surface of cells and is applicable to the super- resolution imaging techniques such as PALM and STORM. Equipped with Nikon Perfect Focus System 4, temperature controlling enclosure and CO2, makes it ideal for long term live cell imaging. The fast sCMOS camera and high numerical aperture objective lens (NA 1.49) makes it an ideal instrument for 3-color super-resolution imaging in 2D and 3D.
Specifications
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Magnification
Numerical Aperture
Corrections
Immersion
Working Distance
MISC.
20x
0.75
Plan Apochromat
AIR
1 mm
DIC/Pol
40x
1.25
Apochromat
Water
0.2 mm
DIC/Pol
60x
1.49
Apochromat
OIL
0.12 mm
DIC/Pol
100x
1.494
Apochromat
OIL
0.12 mm
DIC/Pol
-
Source
Wavelengths
Output Power
Laser OBIS 405
405 nm
100 mW
Laser OBIS 488
488 nm
100 mW
Laser OBIS 561
561 nm
100 mW
Laser OBIS 640
647 nm
100 mW
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- Quad filter usable with 4 laser (405, 488, 561 and 647 nm) for C-STORM, LP650 nm for N-STORM, mCherry and EGFP filter cube.
-
Hamamatsu OCRA FLASH 4.0 V2, large format, high-speed sCMOS camera
- 82% Q.E.
- 2048 * 2048 pixels
- 6.5um * 6.5um pixel size
Funding agency
- Ramaciotti System Microscopy Initiative
Applications
- EPI/HiLO/TIRF imaging
- Live-cell imaging
- Super-Resolution imaging (N-STORM, PAINT, PALM)
- 3D N-STORM imaging
- Automated variable-angle TIRF imaging.
Instrument location
Katharina Gaus Light Microscopy Facility
Lowy Cancer Research Centre (C25),
Lower Ground, Room LG06,
UNSW Sydney, NSW 2033
Phone: 02 9065 5306
Email: KGlmf@unsw.edu.au
Dr Alex Macmillan
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Email
alex.macmillan@unsw.edu.au
Dr Michael Carnell
-
Email
m.carnell@unsw.edu.au
Parent facility
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