Protein & Biologics Characterisation

Comprehensive Analytics: The key to process and product success
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Incidental photos of processes and instruments in UNSW's recombinant products facility

Description

The Recombinant Products Facility (RPF) offers access to a broad range of advanced techniques and equipment to support your analytical needs. Let us help you achieve reliable and impactful results.

  • In-process controls play a vital role in guiding decisions during process development - helping to define upstream and downstream parameters that ensure optimal process performance and consistent quality attributes.
  • Thorough characterisation of your final product is essential for ensuring consistency and reproducible outcomes.

Below is a list of some of the quality attributes tested and analytical techniques available, contact us if you would like to have more details.

Applications

  • Diagnostics
  • Therapeutics 
  • Drug screening 
  • Biomaterials
  • Synthetic Biology

Quality attributes and techniques available

  •  

    • Total protein and/or DNA concentration:  BCA (Bicinchoninic Acid) Assay, Bradford Assay, Qubit, A280, A260
    • specific protein concentration: ELISA, UV-Vis Spectroscopy
    • Virus Like Particle concentration: Nanoparticle Tracking Analysis (NTA):  Nanosight NS300

     

  • Find the ideal formulation (buffer) in which your product should be stored after purification.

    For preclinical trials evaluate the long-term stability of your product.

    • Nano Differential Scanning Fluorometry (nanoDSF): Prometheus-nanotemper.

    • Analytical (HPLC) and preparative (AKTAs) Size exclusion.
    • SDS-PAGE
    • Analytical (HPLC) and preparative (AKTAs) Size exclusion
    • Mass photometer- Refeyn
    • Intact Mass Analysis (IMA)
    • Capillary electrophoresis SDS (CE-SDS)
    • Mass photometer- Refeyn
    • Intact Mass Analysis (IMA)

    • Multi-Angle Dynamic Light Scattering (MADLS®): Malvern Zetasizer Ultra™
    • Transmission Electron Microscopy (TEM)

    • SDS PAGE / Western Blot
    • LC/MS/MS 

     
    • Endotoxin: Endosafe® nexgen-PTS™ 
    • Host cell DNA: qPCR
    • Host Cell proteins: ELISA
    • Surface Plasmon Resonance (SPR): BiaCore T200 
    • Bio-Layer interferometry (BLI): BLItz
    • Microscale Thermophoresis (MST): Monolith NT.115

Related expertise

Location

Recombinant Products Facility

Level 2 and Level 3
Biological Sciences North Building (D26)
UNSW Sydney, NSW 2033

Phone: 02 9065 6379
Email: h.lebhar@unsw.edu.au

Hélène Lebhar

Manager RPF
  • Phone icon
    Phone
    02 9065 6379
  • Send email envelope icon
    Email
    h.lebhar@unsw.edu.au

Parent facility

Recombinant Products Facility (RPF)

The Recombinant Products Facility (RPF) is a dedicated team of experts who specialise in the production, purification and characterisation of proteins and other biologics, offering essential services for both academic and industry researchers.

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