Protein Expression
Description
Various expression systems—including bacteria, yeast, insect cells, and mammalian cells—can be used to produce recombinant proteins, each with unique advantages and limitations. For example, bacterial systems are cost-effective, require simple media, and are easy to scale up. However, proteins requiring post-translational modifications may benefit from more complex systems.
We offer guidance and advanced screening to optimise expression conditions, including:
Expression system selection and construct design
Evaluate various expression systems and customise gene constructs to suit your requirements.
Bacterial expression conditions
Optimise strains, induction conditions, and media using our micro-bioreactor (24- and 96-well formats for 1–4 mL cultures) or shake flasks in ambient and sub-ambient shaker incubators to achieve maximised expression of your protein in the soluble fraction.
Yeast expression
Identify and screen clones with high-level protein expression in yeast-based systems.
Applications
- Diagnostics
- Therapeutics
- Drug screening
- Biomaterials
- Synthetic Biology
Instruments
Incubators (static and shaking) for the following expression hosts
- Microbial (bacteria/Yeasts)
- Mammalian cells
- Insect cells
Duetz-System
- Screening expression conditions and clones
Location
Recombinant Products Facility
Level 2 and Level 3
Biological Sciences North Building (D26)
UNSW Sydney, NSW 2033
Phone: 02 9065 6379
Email: h.lebhar@unsw.edu.au
Hélène Lebhar
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Phone
02 9065 6379 -
Email
h.lebhar@unsw.edu.au
Parent facility
Explore more instruments, facilities & services
Our infrastructure and expertise are accessible to UNSW students and staff, external researchers, government, and industry.