Protein & Biologics Purification
Description
With over 20 years of experience in protein purification, the Recombinant Products Facility team can assist in developing a tailored, multi-step purification strategy for your protein or biologic. Our services include:
Process Development
Expertise in downstream process development to optimise purification workflows. We access a wide range of chromatography chemistries (affinity, ion exchange, hydrophobic, mixed mode, size exclusion) and chromatography support (resins/membranes/ monoliths) from a variety of suppliers to achieve the best outcomes for your product / process.
In-Process Analytics
Access to a broad range of analytical methods for in-process control during development to ensure process performance is optimal and quality attributes consistent.
Custom Column Packing for Scale-Up
Columns ranging from 5 mL to 5 L can be packed on demand with your chosen resin. We offer a variety of columns and resins for screening, polishing, and larger-scale purification.
Applications
- Diagnostics
- Therapeutics
- Drug screening
- Biomaterials
- Synthetic Biology
Purification process
We offer six chromatography systems (ÄKTAs) with varying levels of complexity. Most are accessible to UNSW staff and students after completing training.
Essential between certain chromatography steps, the RPF team is skilled in dialysis and ultrafiltration, utilising both centrifugation and Tangential Flow Filtration (TFF).
Used to remove aggregates that could damage chromatography media or for your final product, both filtration and depth filtration are available at the RPF.
Equipment
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- Ideal for DoE and scouting during process development
- Column packing
- Operating at Room Temperature will a chilled fraction collector
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- Ideal for process development at sub ambient temperatures
- Versatile fraction collector (96 well plates, 15 and 50 mL tubes)
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- Ideal for beginners and small-scale purification at higher pressure
- Operating at sub ambient temperature
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- Ideal for beginners and small-scale purification at low pressure (less than 0.5 MPa)
- Operating at sub ambient temperature
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- For hollow fiber filter cartridges and 50 cm² cassettes
- Sample volume from 50 to 500 mL
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- For cassettes ranging from 50 cm² to 0.14 m²
- Sample volume from 50 to 500 mL
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- For cassettes ranging from 50 cm² 1,000, and up to 4,500 cm²
- Sample volume from 50 to 20 L
- For cassettes ranging from 50 cm² 1,000, and up to 4,500 cm²
Location
Recombinant Products Facility
Level 2 and Level 3
Biological Sciences North Building (D26)
UNSW Sydney, NSW 2033
Phone: 02 9065 6379
Email: h.lebhar@unsw.edu.au
Hélène Lebhar
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Phone
02 9065 6379 -
Email
h.lebhar@unsw.edu.au
Parent facility
Explore more instruments, facilities & services
Our infrastructure and expertise are accessible to UNSW students and staff, external researchers, government, and industry.