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Research & impact
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Research groups
- Retinal Disease and Clinical Imaging Research Group
- Optical Imaging and Visualisation
- Public Health and Health Systems Research Group
- Myopia Research Group
- Ocular Surface Research (Inc. Dry Eye and Contact Lenses)
- Centre for Eye Health Research Group (CFEH)
- Sensory Processes Research Laboratory
- Eye Research Group
- Vision Loss and Health Ageing Group
- Testing and developing new antimicrobials and overcoming antimicrobial resistance (TADOAR)
- Ocular Pathology Research Group
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Research excellence
- Validation of a novel non-invasive high throughput screening tool for peripheral neuropathy in type 2 diabetes
- Eye infection and contact lens
- Development of novel antimicrobial scaffolds
- Preventing injuries and promoting health, safety and mobility for older people with age-related eye disease
- Biomarkers in dry eye disease
- Publications
- Volunteer in our research studies
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Research groups
- Partner with us
- Optometry clinic
- ORLAB
- About us
- Study areas
- Student life & resources
-
Research & impact
Research groups
- Retinal Disease and Clinical Imaging Research Group
- Optical Imaging and Visualisation
- Public Health and Health Systems Research Group
- Myopia Research Group
- Ocular Surface Research (Inc. Dry Eye and Contact Lenses)
- Centre for Eye Health Research Group (CFEH)
- Sensory Processes Research Laboratory
- Eye Research Group
- Vision Loss and Health Ageing Group
- Testing and developing new antimicrobials and overcoming antimicrobial resistance (TADOAR)
- Ocular Pathology Research Group
Research excellence
- Validation of a novel non-invasive high throughput screening tool for peripheral neuropathy in type 2 diabetes
- Eye infection and contact lens
- Development of novel antimicrobial scaffolds
- Preventing injuries and promoting health, safety and mobility for older people with age-related eye disease
- Biomarkers in dry eye disease
- Partner with us
- Optometry clinic
- ORLAB

High throughput microplate assays to evaluate lipid inducing effects of biological agents on meibomian gland epithelial cells and possibly other lipid-producing cells.
Two spectrophotometric microplate assays with dual staining for either fluorescent Nile red (NR) plus 4,6-diamidino-2-phenylindole (DAPI) or non-fluorescent Oil red O (ORO) plus Crystal violet (CV) were applied and optimised to evaluate the lipid producing capacity of immortalised human meibomian gland epithelial cells (iHMGEC). The microplate assays offer a rapid, high throughput and objective measurement of the amount of lipids in iHMGEC (and potentially other lipid-producing cells) and can be used for screening the effects of biological agents or incubation changes on lipid production in cells in future studies.
Compared to the microscopic imaging method, all measurements of the microplate assays are recorded by the microplate reader, providing unbiased objective results. Microplate assays combined with microscopic observations allow both visualisation of lipid distribution within cells and an objective semi-quantitative estimation of lipid production.