Science

Whole Plasmid Sequencing

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Diagram of an assembled plasmid

The Ramaciotti Centre for Genomics is committed to providing a quality full-length plasmid sequencing service for constructs between 2 and 25kb. All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.

Please see below for information on our service offering. If you still need assistance, please contact us.


If you are a returning customer, please submit your order through the LabLink.

  • Uses Oxford Nanopore Technologies for full-length long read sequencing and generation of highly accurate consensus sequences.

    No primers needed.

    The cost is $30/plasmid. For UNSW internals: no quote is required, simply provide account codes in the submission form; For externals to UNSW: email us to request a quote.
    • >300ng of purified plasmid DNA at a concentration of 15-50ng/ul, in nuclease free water or elution buffer
    • Minimum volume 20ul
    • 260/280: 1.8-2.0
    • 260/230: 2.0-2.2
    • Single plasmid samples only (no mixtures)


  • Submit your order via the Sample Submission Portal.

    Place the samples into the "ONT Plasmid" reception box in the Sanger freezer at Ramaciotti Centre entry on level 2 of E26 UNSW

    OR

    Send samples to our parcel delivery address.


  • Turnaround time is 3-8 business days after successful QC of submitted samples. The data package will include:

    • Analysis using the EPI2ME Clone validation workflow; including FASTA consensus sequence and report on the assembled plasmid. 
    • ONT high-accuracy base called sequencing reads (FASTQ)

     

  • Click here to see an example of our plasmid assembly report.

First time submitting to Ramaciotti? - see full guide to sample submission here

Do not need full-length plasmid sequencing? Learn about our Sanger Sequencing services.