Next generation sequencing
The Ramaciotti Centre for Genomics is a long-established, experienced provider of next generation sequencing services. We have state-of-the-art short and long read NGS technologies with scalable capacity. Our expert team of scientists have extensive experience and we always provide our clients data of the highest quality.
All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
The range of NGS services that we offer is extensive, if there is a technique or technology you are interested in that is not listed on the service menu on the right, contact us as we may be able to offer an alternative solution.
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We can deliver the complete genome sequencing package using our suite of Illumina and Oxford Nanopore Technologies next-generation sequencers. Whether you are sequencing a bacterial, human, or other genome, our experienced team can help you design your project and deliver high quality data. Contact us for a quote or assistance with your project design.
All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
Service Genome type - Description Illumina DNA Prep All genomes Illumina DNA PCR-free Prep All genomes Oxford Nanopore - long read All genomes/Full length plasmid TraDIS sequencing Sequencing of transposon introduced mutations -
Exome, panel or targeted sequencing focuses on specific areas of interest reducing sequencing costs and simplifying analysis. We have experience with a wide range of methods for DNA capture, some of these are listed in the table below. Contact us to discuss your project and can will advise you on the best approach.
All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
Type Capture product Exome Core or comprehensive Targeted / panel Custom or pre-designed (e.g. TruSight Oncology 500) Targeted Amplicon - Illumina or Oxford Nanopore Targeted CRISPR TraDIS sequencing Whole genome sequencing of transposon introduced mutations -
The Ramaciotti Centre for Genomics offers four sequencing-based services for the study of the epigenome:
- ChIP-seq - analysis of histone modifications and protein-DNA interactions on a genome-wide scale.
- Methyl-seq - genome-wide or targeted DNA methylation analysis.
- Nucleosome sequencing (client prepared libraries).
- Whole or targeted DNA and RNA methylation by long read sequencing.
Contact us for further information on these services or for a quote. Please note we also offer methylation-analysis by array.
All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
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We have been providing RNA sequencing services to the research community since 2010 and have experience using a wide variety of RNA sequencing protocols allowing us to tailor our services to your project. We provide excellent project support, assisting you with design and approach, and we always deliver high quality data at a competitive price.
Contact us to discuss your project and we can advise you on the best approach.
All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
Service Description mRNA seq Coding only, stranded Total RNA seq* Coding & non-coding, stranded Total RNA seq - low input Coding & non-coding, stranded - lower starting amount Total RNA seq - depleted Coding & non-coding, stranded - client depleted Small RNA seq Small RNA up to 36nt Direct RNA sequencing Oxford Nanopore - long read RNA capture seq Targeted enrichment of RNA regions of interest * Species supported: human, mouse, rat, plant and bacteria. Globin reduction services also available.
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Single-cell sequencing is more accessible than ever. We offer single-cell RNA sequencing through various microfluidic-free methods, catering to both low- and high-throughput requirements.
Please contact us to explore options that best meet your specific needs or click here for more information on our single-cell sequencing service.
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The Ramaciotti Centre for Genomics provides a range of targeted next-generation sequencing services that enable the taxonomic profiling of mixed communities. These methods use primers designed to target and capture taxonomic regions, followed by next-generation sequencing. Targeted sequencing approaches to study the microbiome are often known as diversity profiling or amplicon, metabarcoding or tag sequencing.
Costs start at $37/sample for our standard microbiome sequencing services.
All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
Microbiome sequencing services -16S/18S/ITS
We have a range of pre-optimised gene region targets ready to go, refer to the table below. The workflow includes PCR to incorporate unique barcodes for each sample, pooling of samples into a single library tube, and sequencing on an Illumina sequencer.
Target Gene region Primers Used by* Bacteria 16S V1-V3 27f - 519r AM, BASE 16S V3-V4 341f-805r 16S V4 515f - 806r EMP 16S full length 27f - 1492r ONT - rapid 16S kit Eukaryote 18S V4 18SV4F_18SV4R AM, MM 18S V9 1391f - EukBr AM, BASE, EMP Eukaryote - Fungi ITS1 ITS1F-ITS2 EMP ITS2 fITS7-ITS4 Archea A16S A2f - 519r AM, BASE * AM - Australian Microbiome project, MM - Marine Microbes project, BASE - Biome of Australia Soil Environments (BASE) project, EMP - Earth Microbiome Project.
The AM, BASE and MM projects are Bioplatforms Australia Initiatives.Custom target sequencing services
If you would like to target genes not listed above we can offer a custom short read approach based on a two-step PCR design. For this you would perform an initial round of PCR using a pair of gene/locus specific primers which include Illumina adapter overhangs and then submit the purified product to us for sequencing. Multiple gene targets can be pooled in a sequencing run if they are size compatible. Custom amplicons can also be sequenced using Oxford Nanopore long read technology.
We would be happy to advise you on our custom target sequencing, please contact us. -
NGS-based metagenomic sequencing can comprehensively sequence and analyse all genetic material in your complex sample. This method can detect very low abundance members of a microbial community that may be missed by other methods providing a means to study microorganisms that are otherwise difficult to analyse. Contact us for a quote or assistance with the design of your project.
All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
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Our Next Generation Sequencing (NGS) service for client-prepared libraries is designed to deliver high-quality data with maximum flexibility. If you’ve already completed your own library preparation, we can seamlessly take your libraries through to sequencing on our latest Illumina platforms.
Why choose this service?
Flexibility: Access our full fleet of sequencing instruments, with the ability to scale projects up or down as needed.
Quality assurance: NATA Accredited to ISO/IEC 17025, ensuring your data meets the highest quality standards.
Cost-effective: Competitive pricing with the added benefit of our discounted reagent costs.
Fast turnaround: Standard kit types sequenced within 3-10 business days.
Expertise: Over 16 years of experience delivering high-quality NGS services.
No ongoing costs: Avoid the burden of service contracts and maintenance associated with owning a sequencer.
Contact us for more information or for a quote.