Science

Next generation sequencing

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The Ramaciotti Centre for Genomics is a long-established, experienced provider of next generation sequencing services. We have state-of-the-art short and long read NGS technologies with scalable capacity. Our expert team of scientists have extensive experience and we always provide our clients data of the highest quality. 

All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.

The range of NGS services that we offer is extensive, if there is a technique or technology you are interested in that is not listed on the service menu on the right, contact us as we may be able to offer an alternative solution.

  • We can deliver the complete genome sequencing package using our suite of Illumina, MGI and Oxford Nanopore next-generation sequencers. Whether you are sequencing a bacterial or a human genome our experienced team can help you design your project and deliver high quality data. Contact us for a quote or assistance with your project design. 

    All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
    Service Genome type - Description
    Illumina DNA Prep All genomes
    Illumina DNA PCR-free Prep All genomes
    Oxford Nanopore - long read  All genomes/Full length plasmid
    TraDIS sequencing Sequencing of transposon introduced mutations

  • Complete plasmid sequencing for constructs between 2kb and 25kb.

    Uses Oxford Nanopore Technologies for full-length long read sequencing to generate high consensus accuracy.

    No primers needed.

    The cost is $35/plasmid. For UNSW internals: no quote is required, simply provide account codes in the submission form; For externals to UNSW: email us to request a quote.

     

    Submit your order via the Sample Submission Portal.

    Place the samples into the "ONT Plasmid" reception box in the Sanger freezer at Ramaciotti Centre entry on level 2 of E26 UNSW

    OR  

    Send samples to our parcel delivery address.  

    For service specific sample submission criteria, see Whole Plasmid Sequencing using Oxford Nanopore Requirements.

     

    Turnaround time is 4-10 business days after successful QC of submitted samples.

    The ONT sequencing data output will be: 

    • Analysis using the EPI2ME Clone validation workflow; output FASTA sequence and report on the assembled plasmid.  
    • Raw ONT data  
    • Run summary: report from the GridION or PromethION run

    Do not need full-length plasmid sequencing? Learn about our Sanger Sequencing services.

  • Exome, panel or targeted sequencing focuses on specific areas of interest reducing sequencing costs and simplifying analysis. We have experience with a wide range of methods for DNA capture, some of these are listed in the table below. Contact us to discuss your project and can will advise you on the best approach.

    All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
    Type Capture product
    Exome Core or comprehensive  
    Targeted / panel Custom or pre-designed
    Targeted  Amplicon - Illumina or Oxford Nanopore
    Targeted  CRISPR
    Tra-DIS sequencing Whole genome sequencing of transposon introduced mutations

  • The Ramaciotti Centre for Genomics offers four services for the study of the epigenome: 

    • ChIP-seq - analysis of histone modifications and protein-DNA interactions on a genome-wide scale.
    • Methyl-seq - genome-wide or targeted DNA methylation analysis.
    • Nucleosome sequencing (client prepared libraries).
    • Whole or targeted DNA and RNA methylation by long read sequencing. 

    Contact us for further information on these services or for a quote.

    All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.

  • We have been providing RNA sequencing services to the research community since 2010 and have experience using a wide variety of RNA sequencing protocols allowing us to tailor our services to your project. We provide excellent project support, assisting you with design and approach, and we always deliver high quality data at a competitive price.

    Contact us to discuss your project and we can advise you on the best approach.

    All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
    Service Description
    mRNA seq Coding only, stranded
    Total RNA seq* Coding & non-coding, stranded
    Total RNA seq - low input Coding & non-coding, stranded - lower starting amount
    Total RNA seq - depleted Coding & non-coding, stranded - client depleted
    Small RNA seq Small RNA up to 36nt
    Direct RNA sequencing Oxford Nanopore - long read
    RNA capture seq Targeted enrichment of RNA regions of interest

    * Species supported: human, mouse, rat, plant and bacteria. Globin reduction services also available.

  • The Centre provides single-cell RNA sequencing through various methods, catering to both low and high-throughput requirements in collaboration with the UNSW Cellular Genomics Future Institute.

    Please contact us to explore options that best meet your specific needs.

  • The Ramaciotti Centre for Genomics provides a range of targeted next-generation sequencing services that enable the taxonomic profiling of mixed communities. These methods use primers designed to target and capture taxonomic regions, followed by next-generation sequencing.  Targeted sequencing approaches to study the microbiome are often known as diversity profiling or amplicon, metabarcoding or tag sequencing.

    Costs start at $37/sample for our standard microbiome sequencing services. 

    All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.

    Microbiome sequencing services -16S/18S/ITS

    We have a range of pre-optimised gene region targets ready to go, refer to the table below. The workflow includes PCR to incorporate unique barcodes for each sample, pooling of samples into a single library tube, and sequencing on an Illumina sequencer. 
     

    Target Gene region Primers Used by*
    Bacteria 16S V1-V3 27f - 519r AM, BASE
      16S V3-V4 341f-805r  
      16S V4 515f - 806r EMP 
      16S full length 27f - 1492r ONT - rapid 16S kit 
    Eukaryote 18S V4 18SV4F_18SV4R AM, MM
      18S V9 1391f - EukBr AM, BASE, EMP
    Eukaryote - Fungi ITS1 ITS1F-ITS2 EMP
      ITS2 fITS7-ITS4  
    Archea A16S A2f - 519r AM, BASE
    * AM - Australian Microbiome project, MM - Marine Microbes project, BASE - Biome of Australia Soil Environments (BASE) project, EMP -  Earth Microbiome Project.

    The AM, BASE and MM projects are Bioplatforms Australia Initiatives.

    Custom target sequencing services

    If you would like to target genes not listed above we can offer a custom short read approach based on a two-step PCR design. For this you would perform an initial round of PCR using a pair of gene/locus specific primers which include Illumina adapter overhangs and then submit the purified product to us for sequencing. Multiple gene targets can be pooled in a sequencing run if they are size compatible. Custom amplicons can also be sequenced using Oxford Nanopore long read technology. 

    We would be happy to advise you on our custom target sequencing, please contact us.

  • NGS-based metagenomic sequencing can comprehensively sequence and analyse all genetic material in your complex sample. This method can detect very low abundance members of a microbial community that may be missed by other methods providing a means to study microorganisms that are otherwise difficult to analyse. Contact us for a quote or assistance with the design of your project.

    All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.

  • Our Client Prepared Library service gives you the option of preparing your own library pools in house and sending them to us for sequencing. Some of the benefits of outsourcing your sequencing to us are:

    Flexibility

    You can choose from any sequencer in our fleet of sequencing instruments, allowing you can scale up or down depending on your current needs.  

    Quality assurance

    NATA Accredited to ISO/IEC 17025 ensuring that you receive data of the highest quality data.

    Cost effective

    Competitive pricing with access to our discount reagent costs. 

    Fast turnaround time

    3-10 business days on standard kit types.

    Expertise

    We have >16 years of experience in NGS service provision. 

    No ongoing costs

    You avoid the ongoing annual service contracts required when you own your own sequencer. 
     

    Contact us for more information or for a quote.